Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JM656_RS03445 Genome accession   NZ_CP069159
Coordinates   715687..716667 (+) Length   326 a.a.
NCBI ID   WP_203360015.1    Uniprot ID   -
Organism   Citrobacter sp. R56     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 710687..721667
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JM656_RS03415 (JM656_03415) ansB 711207..712253 (+) 1047 WP_203360010.1 L-asparaginase 2 -
  JM656_RS03420 (JM656_03420) hemW 712362..713498 (-) 1137 WP_203360011.1 radical SAM family heme chaperone HemW -
  JM656_RS03425 (JM656_03425) - 713491..714084 (-) 594 WP_192613450.1 XTP/dITP diphosphatase -
  JM656_RS03430 (JM656_03430) yggU 714093..714383 (-) 291 WP_203360012.1 DUF167 family protein YggU -
  JM656_RS03435 (JM656_03435) - 714380..714946 (-) 567 WP_203360013.1 YggT family protein -
  JM656_RS03440 (JM656_03440) - 714965..715669 (-) 705 WP_203360014.1 YggS family pyridoxal phosphate-dependent enzyme -
  JM656_RS03445 (JM656_03445) pilT 715687..716667 (+) 981 WP_203360015.1 type IV pilus twitching motility protein PilT Machinery gene
  JM656_RS03450 (JM656_03450) ruvX 716715..717131 (-) 417 WP_203360016.1 Holliday junction resolvase RuvX -
  JM656_RS03455 (JM656_03455) - 717131..717694 (-) 564 WP_203360017.1 YqgE/AlgH family protein -
  JM656_RS03460 (JM656_03460) gshB 717872..718819 (-) 948 WP_203360018.1 glutathione synthase -
  JM656_RS03465 (JM656_03465) rsmE 718832..719563 (-) 732 WP_203360019.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JM656_RS03470 (JM656_03470) endA 719641..720348 (-) 708 WP_203360020.1 deoxyribonuclease I -
  JM656_RS03475 (JM656_03475) - 720443..720940 (-) 498 WP_192613440.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35572.72 Da        Isoelectric Point: 7.1739

>NTDB_id=532521 JM656_RS03445 WP_203360015.1 715687..716667(+) (pilT) [Citrobacter sp. R56]
MNMEEIVALSVKHNVSDLHLCNAWSARWRKRGRVEVAPFTSPDIQALLAQWLSDAQQEQLRKNGQLDFAVSLAGNLRLRA
SAFAHQQGTSLALRLLPASCPILAELQAPPVLTNLLSCENGLILVTGATGSGKSTTLAAMVEYLNQHTEAHILTLEDPIE
YCYASQRCLIQQREVGVHCSSFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLEKDKQDGRVALFELLINTPAAGNLIREGKSHQLPGIIQTGQQMGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=532521 JM656_RS03445 WP_203360015.1 715687..716667(+) (pilT) [Citrobacter sp. R56]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAATGCCTGGTCTGCGCG
TTGGCGAAAACGGGGGCGGGTGGAGGTGGCTCCCTTTACGTCGCCGGACATTCAGGCGCTATTAGCGCAGTGGCTTAGTG
ACGCTCAGCAGGAACAACTGCGGAAAAACGGTCAGCTTGATTTTGCCGTGTCGCTGGCGGGGAACTTGCGGCTACGCGCC
AGCGCTTTTGCTCACCAGCAGGGGACATCGCTGGCGCTGCGGTTGCTACCCGCCTCCTGCCCGATACTGGCGGAACTTCA
GGCACCGCCCGTCTTAACGAACCTTCTGAGCTGCGAGAATGGACTTATTCTGGTGACCGGGGCCACTGGTAGTGGGAAAT
CGACCACTCTGGCGGCGATGGTTGAGTATCTCAACCAGCATACTGAGGCGCATATTCTGACCCTTGAAGACCCCATCGAG
TATTGTTACGCCAGTCAGCGTTGTTTGATCCAGCAGCGCGAGGTCGGTGTACATTGCAGTTCGTTTGCCTCCGGGCTACG
TGCGGCATTGCGGGAAGATCCTGACGTTATTTTACTGGGCGAACTGCGAGACAGCGAAACGATCCGTCTGGCGTTGACCG
CAGCAGAAACAGGGCATCTGGTTCTGGCAACGCTGCACACGCGTGGCGCGGCGCAAGCCATTGAGCGATTGATCGACTCT
TTTCCGGCACAGGAGAAAGATCCGGTACGCAGTCAACTGGCGGGCAGTTTGCGTGCGGTATTGTCGCAAAAACTGGAAAA
AGACAAACAGGACGGGCGCGTGGCGCTGTTTGAGTTACTCATCAATACGCCAGCGGCAGGGAATCTGATCCGGGAAGGGA
AGAGTCACCAACTGCCCGGTATAATCCAGACCGGGCAGCAGATGGGAATGCAGACCTTCGCCCAAAGCCTGCAGCAGCGA
CAGGCGCAAGGGAGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Pseudomonas aeruginosa PAK

46.483

100

0.466
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.566

97.546

0.396
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Acinetobacter baylyi ADP1

37.152

99.08

0.368