Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6I52_RS04280 Genome accession   NZ_CP068180
Coordinates   921955..922992 (-) Length   345 a.a.
NCBI ID   WP_004990994.1    Uniprot ID   A0A2N6VBT8
Organism   Acinetobacter ursingii strain FDAARGOS_1095     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 916955..927992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6I52_RS04250 (I6I52_04250) - 917728..918177 (+) 450 WP_004991008.1 bacteriohemerythrin -
  I6I52_RS04255 (I6I52_04255) - 918340..919425 (+) 1086 WP_004991005.1 hypothetical protein -
  I6I52_RS04260 (I6I52_04260) - 919422..919742 (+) 321 WP_004991003.1 RnfH family protein -
  I6I52_RS04265 (I6I52_04265) - 919786..920184 (-) 399 WP_004991001.1 outer membrane protein assembly factor BamE -
  I6I52_RS04270 (I6I52_04270) fur 920296..920736 (+) 441 WP_004990998.1 ferric iron uptake transcriptional regulator -
  I6I52_RS04275 (I6I52_04275) pilU 920810..921928 (-) 1119 WP_004990996.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6I52_RS04280 (I6I52_04280) pilT 921955..922992 (-) 1038 WP_004990994.1 type IV pilus twitching motility protein PilT Machinery gene
  I6I52_RS04285 (I6I52_04285) - 923116..923802 (+) 687 WP_004990993.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6I52_RS04290 (I6I52_04290) - 923953..924798 (+) 846 WP_004990991.1 undecaprenyl-diphosphate phosphatase -
  I6I52_RS04295 (I6I52_04295) - 924756..925447 (-) 692 Protein_827 HAD family hydrolase -
  I6I52_RS04300 (I6I52_04300) - 925528..926313 (-) 786 WP_004990984.1 transglutaminase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38313.90 Da        Isoelectric Point: 6.5052

>NTDB_id=527852 I6I52_RS04280 WP_004990994.1 921955..922992(-) (pilT) [Acinetobacter ursingii strain FDAARGOS_1095]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMDDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGVISPQTARTAAKSPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=527852 I6I52_RS04280 WP_004990994.1 921955..922992(-) (pilT) [Acinetobacter ursingii strain FDAARGOS_1095]
ATGGATATCACAGAATTACTCGCCTTTTCGGTTAAAAACGGTGCATCAGATTTACATCTTTCCGCAGGAATGCCACCGAT
GATTCGTGTCGATGGTGAGGTTCGTCGTATTAATTTACCAGCACTGGAACACAAAGAAGTTCACCGTCTTGTTTATGACA
TTATGAATGATAAACAACGTCGTGACTTTGAAGAACAACTTGAAACTGACTTTTCCTTTGAAGTTCCAAACATTGCGCGT
TTTCGTGTCAATGCCTTTAACCAAAATCGTGGTGCAGGTGCTGTGTTCCGTACCATTCCGTCTAAAGTTCTCACCATGGA
TGATTTGGGTTTAGGACAAATCTTTAAGGATATTTGTGATTATCCACGTGGTATTGTTCTAGTAACAGGCCCAACGGGTT
CGGGTAAATCAACCACGCTTGCCGCTATGCTAGATTATATCAATGACAATCGTTATGACCATATTTTAACCGTTGAAGAT
CCGATCGAATTTGTACACCAATCCAAAAAGTGCTTGATTAACCAACGTGAAGTACATCGTGATACTCATGGCTTTAATGA
AGCACTACGTTCAGCACTACGTGAAGACCCTGATATTATTCTTGTCGGTGAGATGCGCGACCTTGAAACCATTCGTTTGG
CTTTGACCGCCGCAGAAACGGGTCACTTGGTATTTGGTACATTGCATACTACATCTGCTGCAAAAACCATTGACCGTGTA
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTTCGTGCCATGCTATCTGAATCTTTACAAGCCGTTATTTCTCAAAC
ATTATTGAAGAAAAATGGCGGTGGCCGTGTCGCAGCGCATGAGATCATGATCGGGATACCTGCGATCCGTAACTTAATCC
GTGAAAATAAAGTCGCGCAAATGTACTCTGCTATTCAGACAGGTGCTAACTATGGCATGACCACGCTAGATCAAAGCCTC
AAAACATTGGTTTCAAAAGGCGTGATCAGTCCACAAACTGCGCGAACAGCTGCCAAATCGCCAGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N6VBT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

97.101

100

0.971
  pilT Acinetobacter baumannii D1279779

96.812

100

0.968
  pilT Acinetobacter baumannii strain A118

96.812

100

0.968
  pilT Acinetobacter baylyi ADP1

88.372

99.71

0.881
  pilT Pseudomonas aeruginosa PAK

83.721

99.71

0.835
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.345

97.391

0.675
  pilT Vibrio cholerae strain A1552

69.345

97.391

0.675
  pilT Neisseria meningitidis 8013

67.246

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.513
  pilU Vibrio cholerae strain A1552

41.349

98.841

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.762

98.841

0.403
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397