Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JJN09_RS21210 Genome accession   NZ_CP067412
Coordinates   4701661..4702695 (-) Length   344 a.a.
NCBI ID   WP_249483529.1    Uniprot ID   -
Organism   Pseudomonas sp. HS6     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4696661..4707695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JJN09_RS21185 (JJN09_21160) - 4697200..4698471 (+) 1272 WP_249483519.1 dihydroorotase -
  JJN09_RS29635 (JJN09_21165) - 4698793..4699548 (+) 756 WP_254717457.1 lipid II-degrading bacteriocin -
  JJN09_RS29640 - 4699545..4699778 (+) 234 WP_254717460.1 lipid II-degrading bacteriocin -
  JJN09_RS21195 (JJN09_21170) - 4699819..4700223 (+) 405 WP_249483521.1 hypothetical protein -
  JJN09_RS21200 (JJN09_21175) - 4700303..4700737 (-) 435 WP_249483524.1 TM2 domain-containing protein -
  JJN09_RS21205 (JJN09_21180) - 4700986..4701609 (+) 624 WP_249483526.1 C40 family peptidase -
  JJN09_RS21210 (JJN09_21185) pilT 4701661..4702695 (-) 1035 WP_249483529.1 type IV pilus twitching motility protein PilT Machinery gene
  JJN09_RS21215 (JJN09_21190) - 4702752..4703438 (+) 687 WP_249483531.1 YggS family pyridoxal phosphate-dependent enzyme -
  JJN09_RS21220 (JJN09_21195) proC 4703462..4704280 (+) 819 WP_249483533.1 pyrroline-5-carboxylate reductase -
  JJN09_RS21225 (JJN09_21200) - 4704291..4704881 (+) 591 WP_096817605.1 YggT family protein -
  JJN09_RS21230 (JJN09_21205) - 4704878..4705174 (+) 297 WP_249483535.1 DUF167 domain-containing protein -
  JJN09_RS21235 (JJN09_21210) - 4705417..4706556 (+) 1140 WP_249483537.1 homoserine O-acetyltransferase -
  JJN09_RS21240 (JJN09_21215) metW 4706564..4707184 (+) 621 WP_115079510.1 methionine biosynthesis protein MetW -
  JJN09_RS21245 (JJN09_21220) - 4707211..4707645 (+) 435 WP_249483539.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38027.73 Da        Isoelectric Point: 7.1849

>NTDB_id=525941 JJN09_RS21210 WP_249483529.1 4701661..4702695(-) (pilT) [Pseudomonas sp. HS6]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDTQRVAFEKHLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLAMGDVFRKITDAPRGLVLVTGPTGSGKSTTLAAMIDYLNTHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLLAVVSQTLIKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KDLVTKGLISREHAREKARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=525941 JJN09_RS21210 WP_249483529.1 4701661..4702695(-) (pilT) [Pseudomonas sp. HS6]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAACAGGGCGCTTCCGACCTGCACCTGTCGGCTGGACTGCCGCCGAT
GATCCGCGTGGATGGCGATGTGCGGCGGATCAATCTGCCCGCGCTGGATCACAAACAGGTGCATGAGCTGATCTACGACA
TCATGAACGACACCCAGCGAGTGGCGTTCGAGAAACATCTGGAAACCGACTTTTCCTTCGAAGTCCCGGGTGTGGCGCGC
TTTCGGGTCAACGCGTTCAACCAGAACCGTGGTGCCGGCGCGGTGTTCCGCACCATCCCGTCGAAAGTGCTGAGCATGGA
AGATCTCGCCATGGGCGACGTCTTTCGCAAGATCACCGACGCCCCGCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CCGGCAAGTCCACCACCCTGGCGGCGATGATCGATTACCTCAACACCCACCGTCATCACCACATCCTGACCATCGAAGAC
CCGATCGAATTCGTTCACGAATCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCACCGCGATACCCGCAGTTTCGCCAC
AGCATTGCGTTCGGCACTGCGCGAAGACCCGGACGTGATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATCCGTCTGG
CGTTGACCGCCGCCGAGACCGGGCACTTGGTGTTCGGCACGCTGCACACCACGTCGGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCGGGGGATGAGAAGTCGATGGTGCGCTCGATGCTGTCGGAGTCGCTACTGGCCGTGGTGTCGCAGAC
GCTGATCAAGAAGATCGGCGGCGGCCGGGTGGCGGCGCACGAAATCATGCTGGGCACGTCGGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGGGTCGCTGGGGATGCAGACACTCGACATGTGCCTG
AAGGATCTGGTGACCAAGGGCCTGATCAGTCGCGAACATGCGCGGGAGAAGGCGCGGACACCGGATAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

87.5

100

0.875
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

72.485

98.256

0.712
  pilT Legionella pneumophila strain ERS1305867

72.485

98.256

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

39.71

100

0.398