Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DESMER_RS04810 Genome accession   NC_018515
Coordinates   1042263..1043309 (+) Length   348 a.a.
NCBI ID   WP_014901944.1    Uniprot ID   A0A1G7ZLV6
Organism   Desulfosporosinus meridiei DSM 13257     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1037263..1048309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESMER_RS04780 (Desmer_0994) aroB 1037410..1038501 (+) 1092 WP_014901938.1 3-dehydroquinate synthase -
  DESMER_RS04785 (Desmer_0995) - 1038776..1039117 (+) 342 WP_014901939.1 type II secretion system protein GspG -
  DESMER_RS04790 (Desmer_0996) - 1039211..1039645 (+) 435 WP_014901940.1 hypothetical protein -
  DESMER_RS04795 (Desmer_0997) - 1039632..1040135 (+) 504 WP_014901941.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DESMER_RS04800 (Desmer_0998) - 1040234..1040545 (+) 312 WP_052314864.1 hypothetical protein -
  DESMER_RS04805 (Desmer_0999) - 1040545..1042248 (+) 1704 WP_014901943.1 GspE/PulE family protein -
  DESMER_RS04810 (Desmer_1000) pilT 1042263..1043309 (+) 1047 WP_014901944.1 type IV pilus twitching motility protein PilT Machinery gene
  DESMER_RS04815 (Desmer_1001) - 1043306..1044508 (+) 1203 WP_014901945.1 type II secretion system F family protein -
  DESMER_RS04820 (Desmer_1002) - 1044603..1045088 (+) 486 WP_014901946.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DESMER_RS04825 (Desmer_1003) - 1045188..1045955 (+) 768 WP_014901947.1 A24 family peptidase -
  DESMER_RS04830 (Desmer_1004) - 1045980..1047434 (+) 1455 WP_014901948.1 hypothetical protein -
  DESMER_RS04835 (Desmer_1005) - 1047548..1048063 (+) 516 WP_014901949.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38439.52 Da        Isoelectric Point: 9.1763

>NTDB_id=52529 DESMER_RS04810 WP_014901944.1 1042263..1043309(+) (pilT) [Desulfosporosinus meridiei DSM 13257]
MISFKELLLLAGEKKASDIHLTVESPPVLRMDGSLIPSKMDKLSQADLETFTKALMNETQAKRFAEYGELDMSYSLPGVG
RYRINIFRQRGSIGVVIRLIPFTIPSPQALGLPPVSIELAKLHKGLVLVTGPTGSGKSTTLASLIDYINTTRASHIVTIE
DPIEYLHRHKLSLVNQREVGNDTHSFSNALRAVLRQDPDVILVGEMRDLETIATAVTAAETGHLVFSTLHTNDATQSVDR
MIDVFPPHQQQQIRVQLAAVLQGIMSQQLFPRADHKGRVAAIEVLMATPAVRSLIREGKTHQLPTVIQTNAKLGMQTMDK
AIMELVQKGLVTYDVAQEKLQSPDSMRR

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=52529 DESMER_RS04810 WP_014901944.1 1042263..1043309(+) (pilT) [Desulfosporosinus meridiei DSM 13257]
ATGATTTCATTTAAAGAACTTTTACTTTTAGCTGGAGAAAAGAAGGCTTCAGATATTCATTTGACTGTAGAGAGTCCTCC
GGTTTTGCGGATGGATGGCTCACTGATCCCAAGCAAGATGGATAAACTTTCTCAGGCAGATTTAGAGACCTTTACCAAGG
CTCTGATGAATGAAACTCAGGCCAAACGGTTTGCTGAATATGGTGAATTAGATATGTCCTATAGTTTACCGGGTGTTGGA
CGTTATCGGATTAATATATTTCGGCAAAGGGGATCAATTGGAGTGGTTATTCGCCTGATTCCTTTTACAATCCCAAGTCC
TCAGGCCTTAGGACTTCCTCCCGTCAGCATTGAGCTGGCCAAACTGCACAAAGGATTAGTCTTAGTGACTGGCCCAACCG
GAAGTGGAAAATCCACAACTCTGGCTTCACTTATAGACTATATTAATACGACGAGGGCCAGTCATATTGTGACAATTGAA
GATCCCATTGAGTATTTACACCGACATAAGTTAAGTCTAGTCAATCAACGAGAGGTTGGTAACGATACCCATTCTTTTTC
TAATGCTCTGAGAGCTGTTTTACGTCAAGATCCTGATGTAATCTTAGTTGGAGAGATGCGTGACTTAGAAACTATTGCCA
CAGCGGTAACAGCTGCTGAAACCGGACACTTGGTCTTTAGCACTCTTCATACCAATGATGCCACTCAGTCCGTGGATCGG
ATGATCGATGTATTTCCCCCCCATCAGCAGCAGCAAATTCGTGTACAGTTAGCGGCTGTCTTGCAAGGGATTATGTCTCA
ACAATTATTTCCCAGAGCAGATCACAAAGGCAGAGTTGCAGCCATCGAGGTCTTGATGGCCACTCCGGCGGTACGCAGTT
TGATTAGAGAGGGCAAAACCCATCAGCTTCCGACAGTTATTCAAACAAATGCTAAGTTGGGAATGCAAACAATGGACAAG
GCTATTATGGAATTAGTCCAAAAGGGACTGGTAACGTATGACGTTGCCCAGGAAAAGCTGCAGAGCCCTGATAGCATGCG
AAGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(93-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G7ZLV6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.331

99.713

0.552
  pilT Pseudomonas aeruginosa PAK

48.68

97.989

0.477
  pilT Legionella pneumophila strain ERS1305867

49.55

95.69

0.474
  pilT Legionella pneumophila strain Lp02

49.55

95.69

0.474
  pilT Pseudomonas stutzeri DSM 10701

48.387

97.989

0.474
  pilT Neisseria meningitidis 8013

47.801

97.989

0.468
  pilT Neisseria gonorrhoeae MS11

47.214

97.989

0.463
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.619

96.552

0.46
  pilT Vibrio cholerae strain A1552

47.619

96.552

0.46
  pilT Acinetobacter nosocomialis M2

46.334

97.989

0.454
  pilT Acinetobacter baumannii D1279779

46.334

97.989

0.454
  pilT Acinetobacter baumannii strain A118

46.334

97.989

0.454
  pilT Acinetobacter baylyi ADP1

46.041

97.989

0.451
  pilU Pseudomonas stutzeri DSM 10701

43.642

99.425

0.434
  pilU Acinetobacter baylyi ADP1

40.346

99.713

0.402
  pilU Vibrio cholerae strain A1552

42.296

95.115

0.402

Multiple sequence alignment