Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JGR68_RS02255 Genome accession   NZ_CP067113
Coordinates   473341..474453 (-) Length   370 a.a.
NCBI ID   WP_199360207.1    Uniprot ID   -
Organism   Luteimonas sp. MC1750     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468341..479453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGR68_RS02220 (JGR68_02220) coq7 468469..469116 (-) 648 WP_199360201.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  JGR68_RS02225 (JGR68_02225) rplM 469333..469761 (+) 429 WP_199360202.1 50S ribosomal protein L13 -
  JGR68_RS02230 (JGR68_02230) rpsI 469765..470157 (+) 393 WP_199360203.1 30S ribosomal protein S9 -
  JGR68_RS02240 (JGR68_02240) - 470392..471192 (+) 801 WP_199360204.1 2OG-Fe dioxygenase family protein -
  JGR68_RS02245 (JGR68_02245) - 471401..472390 (+) 990 WP_199360205.1 fumarylacetoacetate hydrolase family protein -
  JGR68_RS02250 (JGR68_02250) maiA 472445..473155 (+) 711 WP_199360206.1 maleylacetoacetate isomerase -
  JGR68_RS02255 (JGR68_02255) pilU 473341..474453 (-) 1113 WP_199360207.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JGR68_RS02260 (JGR68_02260) - 474551..474937 (+) 387 WP_199360208.1 DUF4398 domain-containing protein -
  JGR68_RS02265 (JGR68_02265) - 474934..475524 (+) 591 WP_199360209.1 hypothetical protein -
  JGR68_RS02270 (JGR68_02270) - 475864..476082 (+) 219 WP_132998966.1 YdcH family protein -
  JGR68_RS02275 (JGR68_02275) - 476241..477617 (+) 1377 WP_199360210.1 pyridoxal-phosphate dependent enzyme -
  JGR68_RS02280 (JGR68_02280) - 477701..478897 (+) 1197 WP_199360211.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41188.32 Da        Isoelectric Point: 5.8026

>NTDB_id=525107 JGR68_RS02255 WP_199360207.1 473341..474453(-) (pilU) [Luteimonas sp. MC1750]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMALSLAESGR
FRVNVFKQRGEVGMVIRAIRSSIPSIEELQLPQVLKDIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNTTTSGHILTIED
PIEYLHRHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLALNLRAVVSQRLVVGTDGRRMPATEVLINTPMIRDLLRRGQVHEIKQAMEESLEEGIETFDQCL
YRLYKEGRIERETALRAADSREGLDLKFRLSEGGEGEHDPYADVFDTGHA

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=525107 JGR68_RS02255 WP_199360207.1 473341..474453(-) (pilU) [Luteimonas sp. MC1750]
ATGGACATCGGCTACTTCCTGAAGCTGATGACGGAGAAGAACGCTTCCGACATGTTCCTGACCACCGGCGCGCCGGTGTA
CATCAAGGTCGAAGGCAAGCTCTATCCGCTCGGCAACACCGGCCTGCCGCCCGGCATGGTCAAGAAAATCGCCTATTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAACGCGACCTGGAGCTGAACATGGCACTGTCGCTGGCCGAGTCCGGCCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTCATCCGCGCCATCCGCAGCAGCATCCCCTCGATCGA
GGAGCTGCAGCTGCCGCAGGTGCTGAAGGACATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGACTACCGCAACACCACCACGTCCGGGCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACCGGCACAAGAAATCGATCGTGAACCAGCGCGAGGTCGGCCTGGATACCCATGCGTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTGGACGCGACCACGATGGAGGCCG
CGATCGCCTTCGCCGAGACCGGGCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTGAACCTGCGCGCGGTGGTCAGCCAGCG
CCTGGTGGTGGGCACCGACGGCCGCCGCATGCCGGCGACCGAGGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGAGTCCCTGGAGGAAGGCATCGAGACCTTCGACCAGTGCCTG
TACCGGCTGTACAAGGAAGGCCGGATCGAGCGCGAGACCGCGCTGCGCGCCGCCGACTCGCGCGAAGGGCTGGACCTCAA
GTTCCGCCTGTCCGAGGGCGGGGAGGGCGAGCACGACCCCTACGCGGACGTGTTTGATACCGGGCACGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

97.027

0.508
  pilU Vibrio cholerae strain A1552

48.315

96.216

0.465
  pilU Acinetobacter baylyi ADP1

48.305

95.676

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.802

92.973

0.435
  pilT Pseudomonas aeruginosa PAK

42.604

91.351

0.389
  pilT Acinetobacter baylyi ADP1

41.57

92.973

0.386
  pilT Acinetobacter nosocomialis M2

41.57

92.973

0.386
  pilT Acinetobacter baumannii D1279779

41.57

92.973

0.386
  pilT Acinetobacter baumannii strain A118

41.57

92.973

0.386
  pilT Pseudomonas stutzeri DSM 10701

41.279

92.973

0.384
  pilT Legionella pneumophila strain ERS1305867

41.42

91.351

0.378
  pilT Legionella pneumophila strain Lp02

41.42

91.351

0.378
  pilT Neisseria meningitidis 8013

39.769

93.784

0.373
  pilT Neisseria gonorrhoeae MS11

39.769

93.784

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.481

93.784

0.37
  pilT Vibrio cholerae strain A1552

39.481

93.784

0.37