Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JGR64_RS02265 Genome accession   NZ_CP067112
Coordinates   493195..494310 (-) Length   371 a.a.
NCBI ID   WP_199374911.1    Uniprot ID   -
Organism   Luteimonas sp. MC1572     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 488195..499310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGR64_RS02225 (JGR64_02225) sugE 488447..488764 (+) 318 WP_199374905.1 quaternary ammonium compound efflux SMR transporter SugE -
  JGR64_RS02230 (JGR64_02230) coq7 488801..489442 (-) 642 WP_199374906.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  JGR64_RS02235 (JGR64_02235) rplM 489634..490062 (+) 429 WP_182822926.1 50S ribosomal protein L13 -
  JGR64_RS02240 (JGR64_02240) rpsI 490066..490458 (+) 393 WP_199374907.1 30S ribosomal protein S9 -
  JGR64_RS02250 (JGR64_02250) - 490651..491394 (+) 744 WP_199374908.1 2OG-Fe dioxygenase family protein -
  JGR64_RS02255 (JGR64_02255) - 491461..492450 (+) 990 WP_199374909.1 fumarylacetoacetate hydrolase family protein -
  JGR64_RS02260 (JGR64_02260) maiA 492516..493190 (+) 675 WP_234446981.1 maleylacetoacetate isomerase -
  JGR64_RS02265 (JGR64_02265) pilU 493195..494310 (-) 1116 WP_199374911.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JGR64_RS02270 (JGR64_02270) - 494406..494792 (+) 387 WP_199374912.1 DUF4398 domain-containing protein -
  JGR64_RS02275 (JGR64_02275) - 494789..495421 (+) 633 WP_199374913.1 hypothetical protein -
  JGR64_RS02280 (JGR64_02280) - 495763..495981 (+) 219 WP_199374914.1 YdcH family protein -
  JGR64_RS02285 (JGR64_02285) - 496135..497511 (+) 1377 WP_199374915.1 pyridoxal-phosphate dependent enzyme -
  JGR64_RS02290 (JGR64_02290) - 497583..498779 (+) 1197 WP_199374916.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41332.42 Da        Isoelectric Point: 5.9404

>NTDB_id=525089 JGR64_RS02265 WP_199374911.1 493195..494310(-) (pilU) [Luteimonas sp. MC1572]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGRLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMALSLADSGR
FRVNVFKQRGEVGMVIRSIRSTIPSIEELQLPSVLKEIIMAPRGLVLIVGSTGSGKSTTLASMIDYRNSTTSGHILTIED
PIEYLHRHKKSIVNQREVGIDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHICLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLALNLRSVVSQRLVVGTDGRRMPATEVLINTPMIRDLLRRGQVHEIKQAMEESLEEGMESFDQCL
FRLFKEGRIERETALRAADSRDGLDLKFRLSEGGDEHDPYADVFDTGHASH

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=525089 JGR64_RS02265 WP_199374911.1 493195..494310(-) (pilU) [Luteimonas sp. MC1572]
ATGGACATCGGCTATTTCCTCAAGCTGATGACGGAAAAGAATGCCTCCGACATGTTCCTGACCACCGGCGCGCCGGTGTA
CATCAAGGTCGAAGGCAGGCTTTACCCGCTCGGCAACACCGGCCTGCCTCCCGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCGCTGTCGCTGGCGGACTCGGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTCGGCATGGTGATCCGCTCGATCCGCAGCACCATTCCGTCGATCGA
GGAGCTGCAGCTCCCGTCCGTGCTGAAGGAGATCATCATGGCCCCGCGCGGCCTGGTGCTCATCGTCGGCTCCACCGGCT
CCGGCAAGTCGACCACGCTCGCGTCGATGATCGACTACCGCAACAGCACCACGTCCGGGCACATCCTCACCATCGAGGAT
CCCATCGAATACCTGCACCGGCACAAGAAGTCGATCGTCAACCAGCGCGAGGTCGGCATCGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAGATCCTCGACGCCACCACCATGGAGGCCG
CGATCGCATTCGCCGAAACCGGCCACATCTGCCTGGCGACCCTGCACTCGAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGGCGGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCTCGGTGGTGTCGCAGCG
CCTTGTCGTGGGCACCGATGGCCGCCGCATGCCGGCGACCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTTCACGAGATCAAGCAGGCGATGGAGGAATCGCTGGAGGAAGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGGCTGTTCAAGGAAGGCCGCATCGAGCGCGAGACCGCGCTGCGGGCGGCGGATTCCCGCGACGGCCTGGACCTGAA
GTTCCGCCTGTCCGAAGGCGGCGACGAACACGACCCCTACGCCGACGTCTTCGACACCGGCCACGCCTCGCACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.532

96.765

0.499
  pilU Vibrio cholerae strain A1552

48.034

95.957

0.461
  pilU Acinetobacter baylyi ADP1

47.765

96.496

0.461
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.93

92.722

0.426
  pilT Pseudomonas aeruginosa PAK

42.604

91.105

0.388
  pilT Legionella pneumophila strain ERS1305867

41.42

91.105

0.377
  pilT Legionella pneumophila strain Lp02

41.42

91.105

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.124

91.105

0.375
  pilT Acinetobacter baylyi ADP1

40.882

91.644

0.375
  pilT Acinetobacter baumannii D1279779

40.828

91.105

0.372
  pilT Acinetobacter nosocomialis M2

40.828

91.105

0.372
  pilT Acinetobacter baumannii strain A118

40.828

91.105

0.372
  pilT Neisseria gonorrhoeae MS11

39.481

93.531

0.369
  pilT Neisseria meningitidis 8013

39.481

93.531

0.369
  pilT Vibrio cholerae strain A1552

38.905

93.531

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

38.905

93.531

0.364