Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JH280_RS14795 Genome accession   NZ_CP066922
Coordinates   3384951..3385988 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas campestris strain 5053     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3379951..3390988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH280_RS14775 (JH280_14720) - 3380349..3381612 (+) 1264 Protein_2832 hypothetical protein -
  JH280_RS14780 (JH280_14725) - 3381835..3382734 (+) 900 WP_274347312.1 DUF72 domain-containing protein -
  JH280_RS14785 (JH280_14730) - 3382748..3383422 (-) 675 WP_014508435.1 YitT family protein -
  JH280_RS14790 (JH280_14735) pilU 3383708..3384838 (-) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JH280_RS14795 (JH280_14740) pilT 3384951..3385988 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  JH280_RS14800 (JH280_14745) - 3386275..3386958 (+) 684 WP_274347313.1 YggS family pyridoxal phosphate-dependent enzyme -
  JH280_RS14805 (JH280_14750) proC 3387002..3387862 (+) 861 WP_274347314.1 pyrroline-5-carboxylate reductase -
  JH280_RS14810 (JH280_14755) - 3388403..3388825 (+) 423 WP_103700764.1 HU family DNA-binding protein -
  JH280_RS14815 (JH280_14760) - 3388969..3389559 (+) 591 WP_274347315.1 M23 family metallopeptidase -
  JH280_RS14820 (JH280_14765) - 3389652..3389960 (+) 309 WP_014508439.1 DUF3861 domain-containing protein -
  JH280_RS21800 - 3390014..3390553 (+) 540 WP_337999400.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=523203 JH280_RS14795 WP_003489919.1 3384951..3385988(-) (pilT) [Xanthomonas campestris strain 5053]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=523203 JH280_RS14795 WP_003489919.1 3384951..3385988(-) (pilT) [Xanthomonas campestris strain 5053]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCCTTGGACCATAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCACGT
TTCCGCGTCAATGCGTTCAATCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGTTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTCCGCCAGTTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTTGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGCGACACACATGGCTTCAACGA
GGCGCTGCGTTCGGCCTTGCGCGAAGACCCGGATATCATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CACTGACCGCTGCGGAAACCGGCCATCTGGTGTTCGGTACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATTTCGCAGGC
ACTGCTCAAGAAAGTGGGCGGCGGGCGTACCGCAGCGTGGGAAATCATGGTCGGCACTCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGTAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394