Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GI364_RS17225 Genome accession   NZ_CP066339
Coordinates   3659523..3660536 (-) Length   337 a.a.
NCBI ID   WP_198850463.1    Uniprot ID   A0A7T5ES20
Organism   Alicyclobacillus sp. SO9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3654523..3665536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GI364_RS25480 (GI364_17370) fdhF 3654537..3656444 (+) 1908 WP_370541866.1 formate dehydrogenase subunit alpha -
  GI364_RS17210 (GI364_17375) - 3656810..3657694 (+) 885 WP_198850460.1 hypothetical protein -
  GI364_RS17215 (GI364_17380) - 3657861..3658508 (+) 648 WP_198850461.1 TVP38/TMEM64 family protein -
  GI364_RS17220 (GI364_17385) lgt 3658582..3659421 (-) 840 WP_198850462.1 prolipoprotein diacylglyceryl transferase -
  GI364_RS17225 (GI364_17390) pilT 3659523..3660536 (-) 1014 WP_198850463.1 type IV pilus twitching motility protein PilT Machinery gene
  GI364_RS17230 (GI364_17395) - 3660775..3661332 (+) 558 WP_198850464.1 hypothetical protein -
  GI364_RS17235 (GI364_17400) - 3661354..3661971 (+) 618 WP_198850465.1 SpoIID/LytB domain-containing protein -
  GI364_RS17240 (GI364_17405) - 3662097..3662531 (-) 435 WP_198850466.1 hypothetical protein -
  GI364_RS17245 (GI364_17410) - 3662571..3662930 (-) 360 WP_198850467.1 DsrE family protein -
  GI364_RS17250 (GI364_17415) - 3663312..3664649 (+) 1338 WP_198850468.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 37201.61 Da        Isoelectric Point: 6.5033

>NTDB_id=519376 GI364_RS17225 WP_198850463.1 3659523..3660536(-) (pilT) [Alicyclobacillus sp. SO9]
MLEILQQAVEKHASDLHISVNVPPTYRINGELITEGIALTPDDTESLAKDMMSKDEWIRFSENGELDFSYSVQGLSRFRV
NVYRQRGCVSIAVRVVPNHVPSVEELALPASLTQLTEKTHGLVLVTGPTGSGKSTTLAALIDYLNHHSRKHIITLEDPIE
YLHKHDLSIIDQREVGLDTKGFAPALRASLRQDPDVILVGEMRDLETISTAITAAETGHLVFATMHTGDAVQTIDRIVDV
YPPGQQAQIRVQLAGVLQGVVAQKLVVSSDGKSRVAVMEILINTAAVANLIRTEKIHQIRSTMQTGKSYGMQTFDMHLKE
LQSLGKIAKTRLHSEFF

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=519376 GI364_RS17225 WP_198850463.1 3659523..3660536(-) (pilT) [Alicyclobacillus sp. SO9]
TTGCTGGAGATACTACAACAAGCGGTCGAGAAACATGCGTCGGATTTACATATTTCGGTGAATGTGCCGCCAACTTATCG
GATTAATGGAGAGCTGATAACCGAGGGCATCGCCTTAACTCCAGATGATACGGAAAGCTTGGCCAAGGATATGATGTCAA
AAGACGAATGGATACGTTTCAGTGAAAATGGCGAGTTGGATTTTTCATACAGTGTGCAGGGCCTGTCACGGTTTCGAGTG
AATGTCTACAGACAGCGCGGGTGTGTAAGTATTGCCGTACGGGTGGTCCCTAATCATGTGCCTTCCGTTGAGGAGCTGGC
ACTGCCCGCCAGTCTGACGCAATTGACAGAGAAGACCCACGGCCTTGTGCTGGTCACAGGTCCGACTGGAAGTGGCAAAT
CCACCACTCTCGCTGCCCTTATCGACTATTTGAATCACCACAGCAGAAAACACATCATTACGCTGGAAGATCCCATTGAA
TACTTGCACAAACATGATTTGTCCATCATTGACCAACGTGAGGTGGGCCTGGATACGAAAGGGTTTGCTCCTGCCTTGAG
AGCGTCTTTGCGCCAAGATCCCGATGTCATTTTGGTGGGAGAAATGCGCGATTTAGAGACCATCAGCACGGCTATTACTG
CGGCCGAAACTGGACACCTGGTCTTTGCTACGATGCATACGGGAGATGCGGTGCAGACAATAGACCGAATTGTTGACGTC
TATCCGCCTGGTCAACAGGCGCAAATACGTGTTCAATTGGCCGGGGTTCTGCAGGGTGTGGTGGCACAAAAGCTAGTGGT
CTCGTCAGACGGCAAAAGCCGGGTGGCTGTGATGGAGATACTCATCAACACGGCTGCAGTAGCGAACTTAATTCGAACTG
AAAAGATTCATCAAATTCGTTCTACAATGCAGACGGGCAAATCTTATGGAATGCAGACCTTCGATATGCACCTAAAGGAA
TTACAGTCCTTAGGGAAAATAGCTAAAACCAGACTGCATTCAGAGTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(4-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T5ES20

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.625

94.955

0.481
  pilT Pseudomonas aeruginosa PAK

48.485

97.923

0.475
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.761

99.407

0.475
  pilT Vibrio cholerae strain A1552

47.761

99.407

0.475
  pilT Legionella pneumophila strain Lp02

47.605

99.11

0.472
  pilT Legionella pneumophila strain ERS1305867

47.605

99.11

0.472
  pilT Acinetobacter baylyi ADP1

48.16

96.736

0.466
  pilT Pseudomonas stutzeri DSM 10701

47.561

97.329

0.463
  pilT Acinetobacter nosocomialis M2

47.095

97.033

0.457
  pilT Acinetobacter baumannii D1279779

46.789

97.033

0.454
  pilT Acinetobacter baumannii strain A118

46.789

97.033

0.454
  pilT Neisseria meningitidis 8013

46.646

97.329

0.454
  pilT Neisseria gonorrhoeae MS11

46.341

97.329

0.451
  pilU Vibrio cholerae strain A1552

44.272

95.846

0.424
  pilU Pseudomonas stutzeri DSM 10701

43.252

96.736

0.418
  pilU Acinetobacter baylyi ADP1

40.187

95.252

0.383