Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A3UG_RS19170 Genome accession   NC_018079
Coordinates   4087523..4088503 (-) Length   326 a.a.
NCBI ID   WP_014833224.1    Uniprot ID   -
Organism   Enterobacter cloacae subsp. dissolvens SDM     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4082523..4093503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3UG_RS19140 (A3UG_18905) - 4083399..4083896 (+) 498 WP_014833220.1 SprT family zinc-dependent metalloprotease -
  A3UG_RS19145 (A3UG_18910) endA 4083991..4084698 (+) 708 WP_014833221.1 deoxyribonuclease I -
  A3UG_RS19150 (A3UG_18915) rsmE 4084751..4085482 (+) 732 WP_014833222.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A3UG_RS19155 (A3UG_18920) gshB 4085502..4086449 (+) 948 WP_014833223.1 glutathione synthase -
  A3UG_RS19160 (A3UG_18925) - 4086536..4087096 (+) 561 WP_013098664.1 YqgE/AlgH family protein -
  A3UG_RS19165 (A3UG_18930) ruvX 4087096..4087512 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  A3UG_RS19170 (A3UG_18935) pilT 4087523..4088503 (-) 981 WP_014833224.1 type IV pilus twitching motility protein PilT Machinery gene
  A3UG_RS19175 (A3UG_18940) - 4088521..4089222 (+) 702 WP_014833225.1 YggS family pyridoxal phosphate-dependent enzyme -
  A3UG_RS19180 (A3UG_18945) - 4089244..4089810 (+) 567 WP_013098667.1 YggT family protein -
  A3UG_RS19185 (A3UG_18950) yggU 4089807..4090094 (+) 288 WP_014833226.1 DUF167 family protein YggU -
  A3UG_RS19190 (A3UG_18955) - 4090107..4090700 (+) 594 WP_013098669.1 XTP/dITP diphosphatase -
  A3UG_RS19195 (A3UG_18960) hemW 4090693..4091841 (+) 1149 WP_014833227.1 radical SAM family heme chaperone HemW -
  A3UG_RS23945 - 4091877..4091963 (+) 87 Protein_3801 DUF559 domain-containing protein -
  A3UG_RS19200 (A3UG_18965) - 4092062..4092778 (-) 717 WP_014833228.1 DUF2884 domain-containing protein -
  A3UG_RS19205 (A3UG_18970) - 4092835..4093161 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35640.60 Da        Isoelectric Point: 6.3038

>NTDB_id=51930 A3UG_RS19170 WP_014833224.1 4087523..4088503(-) (pilT) [Enterobacter cloacae subsp. dissolvens SDM]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRAGRLEPAPFPSPDVDALLKTWLNDEQQGTWWASGQVDFAVTLAGNQRLRA
SAFKQMKGNSITLRLLPRVCPQLSALGAPQAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDRSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=51930 A3UG_RS19170 WP_014833224.1 4087523..4088503(-) (pilT) [Enterobacter cloacae subsp. dissolvens SDM]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGCAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGACATGGTGGGCAAGCGGGCAGGTAGATTTTGCTGTGACCCTTGCGGGAAACCAGCGCCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCCATCACGCTGCGCCTGTTACCCCGGGTCTGCCCGCAGCTCTCCGCCCTGGG
CGCTCCCCAGGCTATCCCGGAACTCCTGTCCCACGACAGCGGGCTGATACTGGTGACAGGCGCAACCGGCAGCGGCAAAT
CCACAACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTCGAAGATCCGGTGGAG
TTTGTCTATCAGAGCGAACGTTGTCTGATTCAGCAGCGGGAGATCGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CAGTGCGTTGCGGGAAGATCCCGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACCG
CCGCGGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACCCGCGGCGCCTCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAATCAGCTGGCCGGGAGCCTGCGGGCGGTGCTGGCGCAGAGGCTACTCCC
CGATCTGCAGGGTGGGCGCGTCGCGCTGTATGAGCTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGTATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAATTTTGATCGGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilB Haemophilus influenzae 86-028NP

33.708

100

0.368

Multiple sequence alignment