Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I9H06_RS22840 Genome accession   NZ_CP066267
Coordinates   5033975..5035009 (-) Length   344 a.a.
NCBI ID   WP_024668616.1    Uniprot ID   A0AAE6QDZ6
Organism   Pseudomonas tremae strain PA-1-12B     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5028975..5040009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9H06_RS22810 (I9H06_22700) ruvX 5029148..5029588 (+) 441 WP_005888341.1 Holliday junction resolvase RuvX -
  I9H06_RS22815 (I9H06_22705) pyrR 5029687..5030199 (+) 513 WP_122346981.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  I9H06_RS22820 (I9H06_22710) - 5030212..5031216 (+) 1005 WP_122346982.1 aspartate carbamoyltransferase catalytic subunit -
  I9H06_RS22825 (I9H06_22715) - 5031213..5032484 (+) 1272 WP_053479858.1 dihydroorotase -
  I9H06_RS22830 (I9H06_22720) - 5032557..5032961 (-) 405 WP_024668617.1 TM2 domain-containing protein -
  I9H06_RS22835 (I9H06_22725) - 5033212..5033883 (+) 672 WP_122312956.1 C40 family peptidase -
  I9H06_RS22840 (I9H06_22730) pilT 5033975..5035009 (-) 1035 WP_024668616.1 type IV pilus twitching motility protein PilT Machinery gene
  I9H06_RS22845 (I9H06_22735) - 5035065..5035751 (+) 687 WP_024668615.1 YggS family pyridoxal phosphate-dependent enzyme -
  I9H06_RS22850 (I9H06_22740) proC 5035774..5036592 (+) 819 WP_122347005.1 pyrroline-5-carboxylate reductase -
  I9H06_RS22855 (I9H06_22745) - 5036618..5037208 (+) 591 WP_005888327.1 YggT family protein -
  I9H06_RS22860 (I9H06_22750) - 5037413..5038552 (+) 1140 WP_005888326.1 homoserine O-acetyltransferase -
  I9H06_RS22865 (I9H06_22755) metW 5038560..5039180 (+) 621 WP_005888325.1 methionine biosynthesis protein MetW -
  I9H06_RS22870 (I9H06_22760) rdgB 5039217..5039810 (+) 594 WP_122312957.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37933.76 Da        Isoelectric Point: 7.0226

>NTDB_id=519040 I9H06_RS22840 WP_024668616.1 5033975..5035009(-) (pilT) [Pseudomonas tremae strain PA-1-12B]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGNVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNCNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSVGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=519040 I9H06_RS22840 WP_024668616.1 5033975..5035009(-) (pilT) [Pseudomonas tremae strain PA-1-12B]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAACAGGGCGCGTCGGACTTGCACCTCTCTGCAGGTCTGCCACCGAT
GATTCGTGTCGATGGTGATGTGCGCCGCATCAACCTGCCGCCGCTGGACGCGAAGGAGGTCAAGGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGTCAGGATTTCGAAGAGCGTCTAGAGACTGACTTTTCCTTCGAAGTGCCGGGTGTCGCGCGG
TTTCGGGTCAACGCATTCAACCAGAATCGTGGCGCAGGCGCCGTTTTCCGGACCATTCCTTCAAAAATTCTGAGTATGGA
AGACTTGGGGATGGGCAATGTGTTTCGGAAGATTACCGATGTGGCGCGAGGCCTGATTCTGGTCACCGGGCCGACCGGTT
CAGGCAAGTCGACCACTCTGGCGGCGATGATCGACTACCTCAATTGCAACAAGCATCACCATATCCTGACAATCGAAGAC
CCGATCGAATTCGTCCACGAGTCCAAGAAGTGCCTGGTCAACCAGCGTGAGGTTCATCGTGACACGCTGGGTTTCTCGGA
AGCGCTGCGCTCGGCCCTGCGTGAGGACCCGGATGTGATTCTGGTCGGCGAGATGCGCGACCTTGAGACCATTCGCCTGG
CGTTGACCGCTGCGGAGACTGGTCACCTGGTATTCGGCACCCTGCACACCACGTCGGCGGCAAAGACCATTGACCGGATC
GTCGATGTGTTTCCGGCACAGGAAAAGTCGATGATCCGTTCGATGCTGTCCGAATCGCTGCACGCCGTGGTATCGCAGGC
GTTGCTCAAGAAGGTTGGCGGAGGACGCGTGGCGGCGCACGAAATCATGATGGGCACCCCGGCGATTCGTAACCTGATTC
GTGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCGGGTCGGTCGGTATGCAGACGCTGGACATGTGTCTG
GCCGACTTGGTCAAGAAGGGCCTGATCACCCGCGAGAGCGCACGCGAGCGGGCCAAGGTGCCTGACAATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

78.779

100

0.788
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.941

98.837

0.672
  pilT Neisseria gonorrhoeae MS11

67.647

98.837

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.766

97.965

0.654
  pilT Vibrio cholerae strain A1552

66.766

97.965

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395