Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DESACI_RS16050 Genome accession   NC_018068
Coordinates   3501625..3502671 (-) Length   348 a.a.
NCBI ID   WP_014828250.1    Uniprot ID   I4D8Y7
Organism   Desulfosporosinus acidiphilus SJ4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3496625..3507671
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESACI_RS16025 (Desaci_3361) - 3496976..3497515 (-) 540 WP_014828245.1 hypothetical protein -
  DESACI_RS16030 (Desaci_3362) - 3497548..3498996 (-) 1449 WP_014828246.1 Competence protein A -
  DESACI_RS16035 (Desaci_3363) - 3499020..3499769 (-) 750 WP_041276569.1 A24 family peptidase -
  DESACI_RS16040 (Desaci_3364) - 3499851..3500354 (-) 504 WP_049804132.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DESACI_RS16045 (Desaci_3365) - 3500429..3501628 (-) 1200 WP_014828249.1 type II secretion system F family protein -
  DESACI_RS16050 (Desaci_3366) pilT 3501625..3502671 (-) 1047 WP_014828250.1 type IV pilus twitching motility protein PilT Machinery gene
  DESACI_RS16055 (Desaci_3367) - 3502686..3504377 (-) 1692 WP_083845607.1 GspE/PulE family protein -
  DESACI_RS16060 (Desaci_3368) - 3504341..3504679 (-) 339 WP_242833070.1 general secretion pathway protein GspK -
  DESACI_RS16065 (Desaci_3369) - 3504697..3505065 (-) 369 WP_242833071.1 hypothetical protein -
  DESACI_RS16070 (Desaci_3370) - 3505265..3505603 (-) 339 WP_242833072.1 hypothetical protein -
  DESACI_RS16075 (Desaci_3371) - 3505783..3506139 (-) 357 WP_014828254.1 type II secretion system protein GspG -
  DESACI_RS16080 (Desaci_3372) aroB 3506443..3507537 (-) 1095 WP_014828255.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38788.67 Da        Isoelectric Point: 6.5886

>NTDB_id=51832 DESACI_RS16050 WP_014828250.1 3501625..3502671(-) (pilT) [Desulfosporosinus acidiphilus SJ4]
MLLLEELCQVAAEKRASDIHLTAESPPVLRVDGSLMPLGTDKLSSLDLETFTYSLMNETQRKRFLELGELDFSYSLPRVG
RYRVNAYRQRGSIGFVIRLIPYVIPTPESLGLPEVSLDLARLTKGLVLVTGPTGSGKSTTLASLIDYINRTRSCHIMTIE
DPIEYLHKHQLSLVNQREIGCDTNSFSLALRSVLRQDPDVILVGEMRDLETISTAVTAAETGHLVFSTLHTNDATQSVDR
MIDVFPPYQQQQIRVQLAAVLQGIISQQLFPRADHQGRVAAIEVMLATPAIRSLIREGKTHQLPTVIQTNSKLGMQTMDK
AILDLVHKGLVEYDVVQEKLQSADGFRR

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=51832 DESACI_RS16050 WP_014828250.1 3501625..3502671(-) (pilT) [Desulfosporosinus acidiphilus SJ4]
GTGCTTTTGTTGGAAGAACTTTGTCAAGTCGCTGCAGAAAAGAGGGCGTCCGATATCCATTTAACAGCAGAAAGCCCTCC
GGTTCTTCGGGTCGATGGTTCTTTGATGCCGTTAGGAACGGATAAACTGTCTTCACTTGATTTAGAAACCTTTACCTATT
CCCTAATGAACGAGACCCAAAGAAAACGCTTCCTGGAATTAGGAGAGTTAGATTTTTCATATAGTTTGCCGAGAGTAGGG
CGATATCGGGTCAATGCTTATCGGCAAAGAGGCTCTATCGGATTTGTTATTCGCTTGATTCCTTATGTCATTCCTACTCC
GGAAAGTCTCGGTCTCCCGGAAGTGAGCCTTGACTTAGCCCGCTTGACTAAAGGCTTAGTTCTGGTAACAGGTCCCACCG
GGAGTGGAAAATCCACAACTCTTGCCTCCTTGATTGACTATATTAACCGGACGAGATCTTGTCACATTATGACGATTGAA
GATCCCATTGAGTATTTACATAAGCATCAATTAAGTTTAGTTAATCAACGGGAGATTGGCTGCGATACAAATTCTTTTTC
TCTGGCTTTGCGCTCAGTTCTGCGGCAGGATCCCGATGTGATTTTAGTAGGAGAAATGAGGGATCTAGAAACGATTTCCA
CTGCAGTGACAGCAGCTGAAACCGGCCACTTAGTCTTTAGTACTCTGCATACCAATGACGCCACTCAATCAGTAGATCGA
ATGATTGACGTTTTTCCGCCTTATCAGCAGCAACAAATTCGCGTTCAGCTGGCTGCGGTGCTGCAAGGTATTATATCTCA
GCAGCTATTTCCAAGAGCTGATCATCAGGGGCGGGTTGCAGCCATTGAGGTCATGCTGGCAACTCCGGCAATTCGCAGTC
TTATTCGCGAGGGCAAAACTCATCAACTCCCCACAGTGATTCAGACAAACAGTAAACTTGGTATGCAAACTATGGACAAG
GCCATTCTTGACCTTGTTCACAAAGGATTGGTAGAGTATGACGTTGTTCAGGAAAAACTGCAAAGTGCTGATGGTTTTCG
GAGATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(119-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4D8Y7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.783

99.138

0.543
  pilT Pseudomonas aeruginosa PAK

51.02

98.563

0.503
  pilT Pseudomonas stutzeri DSM 10701

50.437

98.563

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.888

97.126

0.494
  pilT Vibrio cholerae strain A1552

50.888

97.126

0.494
  pilT Legionella pneumophila strain Lp02

49.254

96.264

0.474
  pilT Legionella pneumophila strain ERS1305867

49.254

96.264

0.474
  pilT Acinetobacter baumannii D1279779

47.813

98.563

0.471
  pilT Acinetobacter nosocomialis M2

47.813

98.563

0.471
  pilT Acinetobacter baumannii strain A118

47.813

98.563

0.471
  pilT Acinetobacter baylyi ADP1

47.813

98.563

0.471
  pilT Neisseria meningitidis 8013

47.214

97.989

0.463
  pilT Neisseria gonorrhoeae MS11

46.628

97.989

0.457
  pilU Pseudomonas stutzeri DSM 10701

45.317

95.115

0.431
  pilU Vibrio cholerae strain A1552

43.03

94.828

0.408
  pilU Acinetobacter baylyi ADP1

41.088

95.115

0.391

Multiple sequence alignment