Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I9018_RS30380 Genome accession   NZ_CP066123
Coordinates   6605503..6606537 (-) Length   344 a.a.
NCBI ID   WP_241108229.1    Uniprot ID   -
Organism   Pseudomonas sp. MPFS     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6600503..6611537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I9018_RS30350 (I9018_30250) ruvX 6600591..6601028 (+) 438 WP_068576668.1 Holliday junction resolvase RuvX -
  I9018_RS30355 (I9018_30255) pyrR 6601289..6601795 (+) 507 WP_241108227.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  I9018_RS30360 (I9018_30260) - 6601823..6602827 (+) 1005 WP_022641617.1 aspartate carbamoyltransferase catalytic subunit -
  I9018_RS30365 (I9018_30265) - 6602824..6604095 (+) 1272 WP_241108228.1 dihydroorotase -
  I9018_RS30370 (I9018_30270) - 6604165..6604584 (-) 420 WP_068576663.1 TM2 domain-containing protein -
  I9018_RS30375 (I9018_30275) - 6604835..6605458 (+) 624 WP_068576661.1 C40 family peptidase -
  I9018_RS30380 (I9018_30280) pilT 6605503..6606537 (-) 1035 WP_241108229.1 type IV pilus twitching motility protein PilT Machinery gene
  I9018_RS30385 (I9018_30285) - 6606737..6607435 (+) 699 WP_068575486.1 YggS family pyridoxal phosphate-dependent enzyme -
  I9018_RS30390 (I9018_30290) proC 6607469..6608287 (+) 819 WP_068575485.1 pyrroline-5-carboxylate reductase -
  I9018_RS30395 (I9018_30295) - 6608312..6608899 (+) 588 WP_068575484.1 YggT family protein -
  I9018_RS30400 (I9018_30300) - 6608899..6609192 (+) 294 WP_241108230.1 DUF167 domain-containing protein -
  I9018_RS30405 (I9018_30305) - 6609298..6610437 (+) 1140 WP_068575482.1 homoserine O-acetyltransferase -
  I9018_RS30410 (I9018_30310) metW 6610445..6611065 (+) 621 WP_068575481.1 methionine biosynthesis protein MetW -
  I9018_RS30415 (I9018_30315) - 6611092..6611526 (+) 435 WP_068575480.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37244.16 Da        Isoelectric Point: 6.9709

>NTDB_id=517557 I9018_RS30380 WP_241108229.1 6605503..6606537(-) (pilT) [Pseudomonas sp. MPFS]
MDITELLLLSVSRGASDLHLSAGLAPRLRIDGDICPLDSPALEGPQVLAMIHALMSQAQRDAFASLQDLDFAYELPGVAR
FRVNAFQHARGAGAVLRLVPSQVQSLEVLGLGQVLRQIAEVPRGLVLVTGPTGSGKSTTLAAMIDHLNRSRRQHIITIED
PIEFIHPQQACLVHQREVQRDTRSFASALRAALREDPDVILLGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVIAQVLLKRVGGGRVAACEIMLGTVAIRNLIREDKVAQMYSAIQTGGAQGMQTLDMSL
KMLVGQGLISREQAREKARVPEGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=517557 I9018_RS30380 WP_241108229.1 6605503..6606537(-) (pilT) [Pseudomonas sp. MPFS]
ATGGACATCACCGAGCTGCTGCTCTTGAGCGTTTCCCGAGGCGCTTCCGACCTGCACCTATCGGCTGGCCTGGCACCGCG
GTTGCGCATCGATGGCGATATCTGCCCGCTGGATAGCCCGGCCCTGGAAGGGCCGCAAGTACTGGCCATGATCCACGCCC
TGATGAGCCAGGCGCAACGTGACGCCTTTGCCAGCCTCCAGGATCTGGACTTCGCCTATGAACTGCCGGGCGTGGCCCGA
TTCAGGGTCAATGCCTTCCAGCATGCCCGGGGCGCGGGAGCGGTGTTGCGCCTGGTGCCTTCGCAGGTGCAGAGCCTGGA
GGTGCTGGGCCTGGGCCAGGTGCTCCGGCAAATCGCCGAGGTGCCCAGGGGGCTGGTGTTGGTCACCGGGCCTACCGGTA
GCGGCAAGTCCACCACCCTGGCGGCGATGATCGATCATCTGAACCGTAGCCGCCGCCAGCACATCATCACCATCGAGGAC
CCCATCGAGTTCATCCATCCGCAACAGGCATGCCTGGTGCATCAGCGTGAAGTCCAACGCGATACCCGAAGCTTCGCCAG
CGCCTTGCGTGCGGCCTTGCGCGAAGATCCGGATGTGATCCTGCTGGGAGAGCTCCGGGATCTTGAGACCATTCGTTTGG
CGTTGACCGCTGCGGAAACCGGGCACCTGGTGTTCGCCACCCTGCACACGTCATCGGCGGCCAAGACCATCGATCGCCTG
GTGGATGTGTTTCCGGGGGAGGAGAAAGCGTTGGTGCGCTCGATGCTGGCCGAGTCGCTACAGGCGGTGATTGCCCAGGT
ATTGCTCAAGCGCGTGGGCGGTGGGCGAGTGGCGGCTTGCGAGATCATGCTGGGGACCGTCGCGATCCGGAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCAATCCAGACCGGTGGCGCACAGGGTATGCAGACCCTGGACATGAGCCTG
AAAATGCTGGTGGGGCAGGGTTTGATCAGCCGCGAGCAAGCGCGGGAGAAGGCACGGGTGCCGGAGGGTTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

69.767

100

0.698
  pilT Pseudomonas stutzeri DSM 10701

68.895

100

0.689
  pilT Acinetobacter nosocomialis M2

64.826

100

0.648
  pilT Acinetobacter baumannii D1279779

64.826

100

0.648
  pilT Acinetobacter baumannii strain A118

64.826

100

0.648
  pilT Acinetobacter baylyi ADP1

63.663

100

0.637
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.315

97.965

0.61
  pilT Vibrio cholerae strain A1552

62.315

97.965

0.61
  pilT Legionella pneumophila strain Lp02

62.13

98.256

0.61
  pilT Legionella pneumophila strain ERS1305867

62.13

98.256

0.61
  pilT Neisseria meningitidis 8013

57.726

99.709

0.576
  pilT Neisseria gonorrhoeae MS11

57.434

99.709

0.573
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.71

100

0.398
  pilU Vibrio cholerae strain A1552

39.701

97.384

0.387