Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DESTI_RS15550 Genome accession   NC_018025
Coordinates   3671049..3672149 (-) Length   366 a.a.
NCBI ID   WP_014810922.1    Uniprot ID   I4C894
Organism   Desulfomonile tiedjei DSM 6799     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3666049..3677149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESTI_RS15530 (Desti_3119) - 3666103..3666789 (-) 687 WP_014810918.1 ABC transporter ATP-binding protein -
  DESTI_RS15535 (Desti_3120) - 3666782..3668056 (-) 1275 WP_014810919.1 lipoprotein-releasing ABC transporter permease subunit -
  DESTI_RS15540 (Desti_3121) lysS 3668053..3669564 (-) 1512 WP_014810920.1 lysine--tRNA ligase -
  DESTI_RS15545 (Desti_3122) - 3669787..3671007 (-) 1221 WP_014810921.1 type II secretion system F family protein -
  DESTI_RS15550 (Desti_3123) pilT 3671049..3672149 (-) 1101 WP_014810922.1 type IV pilus twitching motility protein PilT Machinery gene
  DESTI_RS15555 (Desti_3124) pilB 3672258..3673952 (-) 1695 WP_014810923.1 type IV-A pilus assembly ATPase PilB -
  DESTI_RS15560 (Desti_3125) - 3674128..3675249 (+) 1122 WP_014810924.1 DUF362 domain-containing protein -
  DESTI_RS15565 (Desti_3126) - 3675676..3676482 (+) 807 WP_014810925.1 hypothetical protein -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41098.55 Da        Isoelectric Point: 8.0988

>NTDB_id=51744 DESTI_RS15550 WP_014810922.1 3671049..3672149(-) (pilT) [Desulfomonile tiedjei DSM 6799]
MASLHDFLKEMVERGASDLHVTPGVPPMIRVRGELVPLEYPPLKPQETRQLCYSILTDVQKHRFEENQELDLSFGVRGLS
RFRANIFMQRGALAGAFRLIPYQIRSFQELGLPPIVNDLCAKPRGLVLVTGPTGSGKSTTLAAMIDKINSDRREHIVTIE
DPIEFTHQHKSCIVNQREVHQDTKSFHAALRSILRQDPDVVLIGEMRDLETIEAALTVSETGHLTFGTLHTNSCVQTINR
IIDVFPAHQQPQVRAQLSFVLEGVMCQTLIPTLDGRGRTLSLEIMVPNAAIRNLIREDKVHQIYSQIQVGQAKSGMQTMN
QSLLMLYQRRIISFDDAMARSPDTDELKQMMSGLPKAAPKKIIRPG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=51744 DESTI_RS15550 WP_014810922.1 3671049..3672149(-) (pilT) [Desulfomonile tiedjei DSM 6799]
TTGGCAAGTCTCCATGATTTTTTGAAGGAAATGGTTGAGCGAGGCGCTTCAGACCTCCACGTGACACCAGGTGTCCCGCC
TATGATCCGAGTTCGTGGTGAACTCGTTCCTCTCGAATATCCGCCGCTGAAACCTCAGGAAACCAGGCAGCTTTGCTACA
GTATTCTCACGGATGTCCAAAAACATCGATTCGAAGAGAACCAGGAGCTTGACCTCTCCTTTGGAGTTCGAGGTCTGAGC
CGGTTCAGAGCGAACATATTTATGCAGCGTGGGGCGCTGGCAGGAGCATTCAGGCTTATTCCCTACCAGATTCGTTCTTT
TCAGGAACTGGGATTACCGCCTATTGTGAACGATCTGTGCGCGAAACCACGGGGACTCGTTCTGGTGACCGGGCCGACGG
GCTCCGGTAAATCGACCACTCTGGCTGCCATGATCGATAAGATAAACAGTGACCGTCGCGAGCACATCGTCACCATTGAA
GATCCCATCGAATTTACCCATCAACACAAGTCTTGCATCGTGAATCAGCGGGAGGTTCACCAGGATACCAAGAGCTTTCA
TGCGGCGCTTCGGTCGATTCTCCGCCAGGATCCTGACGTCGTGCTCATCGGAGAAATGAGAGACCTGGAGACTATCGAGG
CTGCGTTGACCGTTTCCGAAACGGGCCACCTGACATTCGGAACTCTCCATACGAACTCGTGTGTGCAGACGATAAACCGA
ATTATCGACGTCTTTCCCGCGCATCAGCAGCCGCAGGTTCGGGCACAACTCAGCTTCGTTCTTGAAGGGGTAATGTGTCA
AACTCTGATACCGACACTCGACGGTCGGGGCCGCACGCTTTCCCTGGAAATCATGGTTCCGAATGCTGCTATCCGGAATC
TCATTCGTGAAGATAAAGTCCACCAGATTTATTCTCAGATTCAAGTCGGTCAGGCGAAATCTGGAATGCAGACTATGAAT
CAGTCGCTTCTGATGCTTTATCAAAGGAGAATAATCTCGTTTGACGATGCAATGGCCAGGTCTCCCGATACAGACGAACT
CAAACAGATGATGTCCGGCCTGCCAAAGGCTGCACCCAAGAAGATCATCAGACCCGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4C894

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

58.075

87.978

0.511
  pilT Legionella pneumophila strain Lp02

58.075

87.978

0.511
  pilT Acinetobacter baylyi ADP1

54.252

93.169

0.505
  pilT Acinetobacter baumannii D1279779

54.878

89.617

0.492
  pilT Acinetobacter baumannii strain A118

54.878

89.617

0.492
  pilT Acinetobacter nosocomialis M2

54.878

89.617

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.585

94.809

0.489
  pilT Pseudomonas aeruginosa PAK

52.381

91.803

0.481
  pilT Pseudomonas stutzeri DSM 10701

51.488

91.803

0.473
  pilT Neisseria meningitidis 8013

50

93.443

0.467
  pilT Neisseria gonorrhoeae MS11

49.708

93.443

0.464
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

89.344

0.454
  pilT Vibrio cholerae strain A1552

50.765

89.344

0.454
  pilU Pseudomonas stutzeri DSM 10701

42.319

94.262

0.399
  pilU Acinetobacter baylyi ADP1

40.909

96.175

0.393
  pilU Vibrio cholerae strain A1552

40.517

95.082

0.385

Multiple sequence alignment