Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DESTI_RS11870 Genome accession   NC_018025
Coordinates   2827134..2828204 (+) Length   356 a.a.
NCBI ID   WP_014810202.1    Uniprot ID   I4C668
Organism   Desulfomonile tiedjei DSM 6799     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2822134..2833204
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESTI_RS11845 (Desti_2370) - 2822249..2822704 (+) 456 WP_041286167.1 hypothetical protein -
  DESTI_RS11850 (Desti_2371) - 2822777..2823343 (-) 567 WP_014810198.1 cob(I)yrinic acid a,c-diamide adenosyltransferase -
  DESTI_RS11855 (Desti_2372) - 2823599..2823964 (-) 366 WP_014810199.1 hypothetical protein -
  DESTI_RS28790 (Desti_2373) - 2824382..2825947 (+) 1566 WP_014810200.1 response regulator -
  DESTI_RS11865 (Desti_2374) pilT 2826081..2827124 (+) 1044 WP_014810201.1 type IV pilus twitching motility protein PilT Machinery gene
  DESTI_RS11870 (Desti_2375) pilT 2827134..2828204 (+) 1071 WP_014810202.1 type IV pilus twitching motility protein PilT Machinery gene
  DESTI_RS29965 (Desti_2376) - 2828444..2829370 (-) 927 WP_014810203.1 glycyl-radical enzyme activating protein -
  DESTI_RS11880 (Desti_2377) - 2829773..2830219 (-) 447 WP_014810204.1 hypothetical protein -
  DESTI_RS28805 (Desti_2378) - 2830267..2832825 (-) 2559 WP_014810205.1 pyruvate formate lyase family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39026.32 Da        Isoelectric Point: 7.8920

>NTDB_id=51742 DESTI_RS11870 WP_014810202.1 2827134..2828204(+) (pilT) [Desulfomonile tiedjei DSM 6799]
MPKIDSLLTEMIQSGASDLHMVPGMPPLLRLKGDLVPTKHKPLSHKLNEALFFEMMTSQQQKVLQTTLELDLAHEIVGLA
RFRCNYFYQRRGISAVFRQIPTRIKSLEELGMPAGVHNILGIKKGLVLVTGPTGSGKSTTLAAMIDYINRNEEKHIITIE
DPLEFVHENQKSLITQREIGAHAKSFADALRVASREDPNIILVGEMRDLETIALALSSAELGILVFGTVHTNSAVKTIDR
VINAFPASQQNQVRTMLSDSLKAVLAQQLLKTADGKGRCAAVEILISNSAVGNLIREGKLAQIASIIQTGSLEGMQTMDQ
ALQQLVDQKRITPEAAYWKAIDKSIFAAQCPAGFVE

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=51742 DESTI_RS11870 WP_014810202.1 2827134..2828204(+) (pilT) [Desulfomonile tiedjei DSM 6799]
TTGCCCAAAATAGACAGTCTACTCACTGAGATGATTCAATCCGGGGCCTCGGACCTGCACATGGTCCCTGGAATGCCTCC
TCTGTTGCGACTCAAAGGGGATTTAGTTCCTACCAAACACAAGCCGCTCTCGCACAAACTGAATGAGGCCCTCTTTTTTG
AGATGATGACCAGCCAGCAGCAGAAGGTTCTGCAGACAACCCTTGAACTTGACCTTGCGCATGAAATCGTCGGCTTGGCT
CGTTTCAGATGCAATTATTTCTACCAACGCAGGGGTATATCCGCGGTATTTCGCCAAATCCCCACCAGGATCAAGTCGCT
TGAAGAACTTGGCATGCCGGCTGGGGTTCACAATATTCTTGGAATCAAGAAGGGATTGGTCCTGGTGACCGGTCCGACAG
GATCTGGCAAGTCCACCACGCTTGCTGCCATGATTGACTACATAAACAGAAACGAAGAAAAACACATCATTACCATCGAA
GATCCGCTTGAATTCGTTCACGAGAACCAAAAGTCCCTCATCACCCAGCGTGAGATAGGTGCCCACGCGAAAAGCTTTGC
CGATGCTCTTCGAGTAGCGTCAAGGGAAGATCCGAACATCATTCTTGTAGGTGAGATGCGGGACCTGGAAACCATTGCGC
TTGCCCTCAGCAGCGCAGAATTGGGAATTCTGGTCTTCGGCACGGTCCACACCAACTCGGCCGTAAAGACCATCGACCGG
GTTATAAATGCTTTCCCTGCAAGCCAGCAGAATCAGGTTCGCACCATGTTGAGCGATTCCCTCAAGGCGGTGCTTGCTCA
ACAGCTTCTCAAGACTGCAGACGGCAAAGGAAGGTGTGCTGCTGTAGAAATACTGATCAGCAATTCCGCTGTGGGAAATC
TCATCCGGGAAGGAAAGCTTGCTCAAATAGCTTCGATCATCCAGACAGGAAGCTTGGAAGGCATGCAGACAATGGATCAG
GCGCTGCAACAGCTTGTGGATCAGAAGCGCATTACCCCCGAAGCGGCTTATTGGAAAGCAATCGATAAATCGATTTTTGC
CGCCCAATGTCCAGCCGGATTCGTAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4C668

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

54.142

94.944

0.514
  pilT Neisseria meningitidis 8013

54.142

94.944

0.514
  pilT Legionella pneumophila strain ERS1305867

53.061

96.348

0.511
  pilT Legionella pneumophila strain Lp02

53.061

96.348

0.511
  pilT Pseudomonas aeruginosa PAK

53.709

94.663

0.508
  pilT Pseudomonas stutzeri DSM 10701

53.116

94.663

0.503
  pilT Acinetobacter baumannii strain A118

52.819

94.663

0.5
  pilT Acinetobacter nosocomialis M2

52.819

94.663

0.5
  pilT Acinetobacter baumannii D1279779

52.819

94.663

0.5
  pilT Acinetobacter baylyi ADP1

54.489

90.73

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.87

90.73

0.489
  pilT Vibrio cholerae strain A1552

53.87

90.73

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.419

96.629

0.478
  pilU Pseudomonas stutzeri DSM 10701

42.012

94.944

0.399
  pilU Vibrio cholerae strain A1552

40.237

94.944

0.382
  pilU Acinetobacter baylyi ADP1

38.824

95.506

0.371

Multiple sequence alignment