Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6H86_RS17005 Genome accession   NZ_CP066089
Coordinates   3624036..3625016 (+) Length   326 a.a.
NCBI ID   WP_012135066.1    Uniprot ID   -
Organism   Citrobacter koseri strain FDAARGOS_1029     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3619036..3630016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6H86_RS16975 (I6H86_16975) ansB 3619547..3620593 (+) 1047 WP_012135072.1 L-asparaginase 2 -
  I6H86_RS16980 (I6H86_16980) hemW 3620712..3621848 (-) 1137 WP_012135071.1 radical SAM family heme chaperone HemW -
  I6H86_RS16985 (I6H86_16985) - 3621841..3622434 (-) 594 WP_012135070.1 XTP/dITP diphosphatase -
  I6H86_RS16990 (I6H86_16990) yggU 3622442..3622732 (-) 291 WP_012135069.1 DUF167 family protein YggU -
  I6H86_RS16995 (I6H86_16995) - 3622729..3623295 (-) 567 WP_012135068.1 YggT family protein -
  I6H86_RS17000 (I6H86_17000) - 3623314..3624018 (-) 705 WP_012135067.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6H86_RS17005 (I6H86_17005) pilT 3624036..3625016 (+) 981 WP_012135066.1 type IV pilus twitching motility protein PilT Machinery gene
  I6H86_RS17010 (I6H86_17010) ruvX 3625159..3625575 (-) 417 WP_012135064.1 Holliday junction resolvase RuvX -
  I6H86_RS17015 (I6H86_17015) - 3625575..3626138 (-) 564 WP_012135063.1 YqgE/AlgH family protein -
  I6H86_RS17020 (I6H86_17020) gshB 3626315..3627262 (-) 948 WP_012135062.1 glutathione synthase -
  I6H86_RS17025 (I6H86_17025) rsmE 3627275..3628006 (-) 732 WP_012135061.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6H86_RS17030 (I6H86_17030) endA 3628084..3628791 (-) 708 WP_012135060.1 deoxyribonuclease I -
  I6H86_RS17035 (I6H86_17035) - 3628886..3629383 (-) 498 WP_024130936.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35594.65 Da        Isoelectric Point: 7.0594

>NTDB_id=517289 I6H86_RS17005 WP_012135066.1 3624036..3625016(+) (pilT) [Citrobacter koseri strain FDAARGOS_1029]
MDMEEIVALSVKHNVSDLHLCNAWPARWRKRGKVEIAPFSSPNVEVLLSQWLNDAQRRAWQADGQLDFAVSLPGGLRLRA
SAFHHEQGASLALRLLPTTCPQLDALQTPAALPALLGGENGLILVTGATGSGKSTTLAAMVEYLNQHLDGHILTLEDPIE
YRHTSQRCLIQQREVGVHCRSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLLLATLHTRGAAQAVERLVDS
FPAQEKDPVRSQLAGSLRAVLSQKLETDRQDGRVALFELLINTPAVGNLIREGKIHQLPGVIQTSQQAGMQTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=517289 I6H86_RS17005 WP_012135066.1 3624036..3625016(+) (pilT) [Citrobacter koseri strain FDAARGOS_1029]
ATGGATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAACGCATGGCCTGCGCG
CTGGCGTAAACGGGGAAAAGTGGAAATAGCCCCGTTTAGCAGCCCGAATGTCGAGGTTTTGCTGTCACAATGGCTTAACG
ACGCGCAGCGCCGGGCGTGGCAGGCGGATGGTCAGCTCGACTTTGCGGTGTCGCTGCCGGGCGGACTGCGGTTACGCGCC
AGCGCTTTTCATCACGAGCAGGGCGCATCGCTGGCGCTGCGGTTGCTGCCCACTACGTGCCCGCAACTGGACGCGCTCCA
GACGCCTGCCGCGCTACCCGCGCTCCTTGGCGGCGAGAACGGACTGATTCTGGTGACCGGGGCGACGGGAAGCGGGAAAT
CCACCACGCTGGCGGCAATGGTAGAATACCTCAACCAGCATCTCGATGGTCATATCCTGACGCTGGAAGATCCTATTGAG
TACCGCCATACCAGCCAGCGCTGCCTGATTCAGCAGCGGGAGGTTGGTGTACACTGCCGCTCCTTTGCCGCCGGGTTACG
CGCTGCGCTGCGTGAAGACCCGGATGTGATTTTATTAGGCGAACTGCGCGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGTTACTGGCGACGCTACATACGCGCGGCGCGGCACAGGCGGTAGAGCGGCTGGTTGACTCC
TTTCCGGCGCAGGAGAAAGATCCGGTACGCAGTCAACTTGCGGGCAGCCTGCGCGCCGTGTTGTCGCAGAAGCTGGAAAC
CGACAGGCAGGACGGACGCGTGGCGTTATTCGAACTGCTCATCAACACGCCAGCGGTTGGCAATCTTATTCGGGAGGGGA
AAATTCACCAGTTGCCCGGCGTTATTCAGACCAGCCAACAGGCGGGGATGCAGACGTTTGCCCAGAGTCTGCAACAGCGG
CAGGCGCAGGGCAGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(50-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

46.273

98.773

0.457
  pilT Legionella pneumophila strain Lp02

46.273

98.773

0.457
  pilU Pseudomonas stutzeri DSM 10701

40.909

100

0.414
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

40.062

98.773

0.396
  pilU Acinetobacter baylyi ADP1

38.272

99.387

0.38