Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TSAC_RS09910 Genome accession   NC_017992
Coordinates   2004730..2005782 (-) Length   350 a.a.
NCBI ID   WP_014758813.1    Uniprot ID   -
Organism   Thermoanaerobacterium saccharolyticum JW/SL-YS485     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1999730..2010782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TSAC_RS09875 (Tsac_1950) - 1999987..2000589 (-) 603 WP_014758806.1 hypothetical protein -
  TSAC_RS09880 (Tsac_1951) - 2000600..2001064 (-) 465 WP_014758807.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TSAC_RS09885 (Tsac_1952) - 2001042..2001779 (-) 738 WP_014758808.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TSAC_RS09890 (Tsac_1953) - 2001791..2002264 (-) 474 WP_014758809.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TSAC_RS09895 (Tsac_1954) - 2002269..2003024 (-) 756 WP_014758810.1 A24 family peptidase -
  TSAC_RS09900 (Tsac_1955) - 2003074..2003463 (-) 390 WP_014758811.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TSAC_RS09905 (Tsac_1956) - 2003489..2004703 (-) 1215 WP_014758812.1 type II secretion system F family protein -
  TSAC_RS09910 (Tsac_1957) pilT 2004730..2005782 (-) 1053 WP_014758813.1 type IV pilus twitching motility protein PilT Machinery gene
  TSAC_RS09915 (Tsac_1958) - 2005811..2007505 (-) 1695 WP_014758814.1 ATPase, T2SS/T4P/T4SS family -
  TSAC_RS09920 (Tsac_1959) aroE 2007659..2008510 (-) 852 WP_014758815.1 shikimate dehydrogenase -
  TSAC_RS09925 (Tsac_1960) - 2008515..2009009 (-) 495 WP_014758816.1 YqeG family HAD IIIA-type phosphatase -
  TSAC_RS09930 (Tsac_1961) sigK 2009092..2009787 (-) 696 WP_014758817.1 RNA polymerase sporulation sigma factor SigK -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38774.04 Da        Isoelectric Point: 6.5053

>NTDB_id=51608 TSAC_RS09910 WP_014758813.1 2004730..2005782(-) (pilT) [Thermoanaerobacterium saccharolyticum JW/SL-YS485]
MKTSELLAMVVEKGASDLHITVGVPPVLRINGQLIKLNLPQLTPQDTEEITKDLLSSDELKKLEDMGDIDLSYSVKGLGR
FRINAYKQRGTYSLAIRSVALRIPTIDELGLPEVIKELALKTRGLIIVTGPTGSGKSTTLASMIDLINEERNCHILTLED
PIEYLHKHKKSIVNQREIGHDAASYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGSAKTIDRI
IDVFPPHQQQQIKVQLSNVLEGIVSQQLLPKIDNSGRVVAVEVMIATPAIRNLIREGKSFQIQSMVQTGNKFGMVTMDMW
ISQLLKRNLISMDDALTYCVDRENFSRLVV

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=51608 TSAC_RS09910 WP_014758813.1 2004730..2005782(-) (pilT) [Thermoanaerobacterium saccharolyticum JW/SL-YS485]
ATGAAAACAAGTGAACTTCTGGCTATGGTTGTAGAAAAAGGCGCATCTGATTTGCATATAACAGTTGGCGTTCCTCCAGT
TCTCAGGATTAATGGTCAACTTATAAAACTGAATTTACCTCAATTAACACCTCAAGATACGGAAGAAATTACAAAAGATT
TGCTTTCAAGCGATGAATTAAAAAAGCTTGAAGATATGGGTGACATTGATTTGTCGTATTCAGTAAAAGGGCTTGGAAGG
TTTAGAATAAATGCGTATAAGCAGAGAGGAACATATAGCCTTGCCATAAGATCTGTCGCTTTGAGAATTCCCACAATTGA
TGAATTAGGACTTCCAGAAGTGATTAAAGAACTTGCGCTTAAAACCCGTGGTCTCATAATTGTTACAGGGCCTACAGGCA
GCGGAAAATCTACGACATTAGCATCTATGATCGACTTAATAAACGAAGAACGCAATTGTCATATATTGACACTTGAAGAC
CCTATTGAATATTTGCATAAGCATAAAAAGAGCATAGTAAATCAAAGAGAAATTGGACATGACGCTGCGTCATACGCCAG
TGCATTAAGGGCAGCATTAAGAGAAGACCCTGATGTGATACTTGTGGGGGAGATGAGAGATCTTGAGACGATACAGATAG
CTATAACAGCAGCTGAGACAGGGCATCTCGTGTTATCCACTTTACATACTATAGGTTCAGCAAAGACTATTGATAGGATT
ATAGATGTTTTTCCACCGCATCAACAGCAACAGATAAAAGTACAGCTTTCAAATGTGTTAGAAGGGATTGTGTCTCAACA
ATTGCTGCCTAAAATAGACAATTCAGGGCGAGTCGTCGCTGTAGAAGTCATGATAGCCACACCTGCAATAAGAAACCTAA
TAAGAGAAGGTAAGTCATTTCAAATTCAATCGATGGTGCAAACCGGAAATAAATTTGGAATGGTGACAATGGACATGTGG
ATATCACAGTTATTAAAGAGGAATTTGATTTCTATGGATGATGCGCTTACGTATTGCGTTGACAGAGAAAACTTTTCTCG
ATTAGTAGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.422

98.857

0.489
  pilT Legionella pneumophila strain ERS1305867

49.275

98.571

0.486
  pilT Legionella pneumophila strain Lp02

49.275

98.571

0.486
  pilT Pseudomonas aeruginosa PAK

48.986

98.571

0.483
  pilT Acinetobacter nosocomialis M2

48.406

98.571

0.477
  pilT Acinetobacter baumannii D1279779

48.406

98.571

0.477
  pilT Acinetobacter baumannii strain A118

48.406

98.571

0.477
  pilT Pseudomonas stutzeri DSM 10701

48.406

98.571

0.477
  pilT Acinetobacter baylyi ADP1

47.826

98.571

0.471
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.182

94.286

0.454
  pilT Vibrio cholerae strain A1552

48.182

94.286

0.454
  pilT Neisseria meningitidis 8013

45.087

98.857

0.446
  pilT Neisseria gonorrhoeae MS11

44.798

98.857

0.443
  pilU Pseudomonas stutzeri DSM 10701

40.857

100

0.409
  pilU Vibrio cholerae strain A1552

39.886

100

0.4
  pilU Acinetobacter baylyi ADP1

38.746

100

0.389

Multiple sequence alignment