Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C6N34_RS10490 Genome accession   NZ_CP065936
Coordinates   2316273..2317349 (+) Length   358 a.a.
NCBI ID   WP_115539360.1    Uniprot ID   -
Organism   Cylindrospermopsis raciborskii Cr2010     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2311273..2322349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6N34_RS10470 (C6N34_010445) - 2311555..2311797 (-) 243 WP_057178779.1 sulfurtransferase TusA family protein -
  C6N34_RS10475 (C6N34_010450) dnaJ 2311807..2312937 (-) 1131 WP_006276280.1 molecular chaperone DnaJ -
  C6N34_RS10480 (C6N34_010455) grpE 2313222..2313917 (-) 696 WP_369806407.1 nucleotide exchange factor GrpE -
  C6N34_RS10485 (C6N34_010460) pilB/pilB1 2314237..2316255 (+) 2019 WP_057178780.1 GspE/PulE family protein Machinery gene
  C6N34_RS10490 (C6N34_010465) pilT 2316273..2317349 (+) 1077 WP_115539360.1 type IV pilus twitching motility protein PilT Machinery gene
  C6N34_RS10495 (C6N34_010470) - 2317392..2318651 (+) 1260 WP_057177258.1 type II secretion system F family protein -
  C6N34_RS10500 (C6N34_010475) - 2318680..2319684 (-) 1005 WP_115539368.1 saccharopine dehydrogenase-like oxidoreductase -
  C6N34_RS10505 (C6N34_010480) - 2320343..2321512 (+) 1170 WP_369806393.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 40022.41 Da        Isoelectric Point: 7.1091

>NTDB_id=515438 C6N34_RS10490 WP_115539360.1 2316273..2317349(+) (pilT) [Cylindrospermopsis raciborskii Cr2010]
MEYTIQELLQNLVDMGGSDLHLSASLRPYFRIGGKLTPIGNEILTPNECERLIFSMLNNDQRHRLEKTWELDCSYEIKAL
ARFRVNVYKERGNYAACLRVLSYKIPNFQELGLPNIVREIAEKPRGLVLVTGPTGSGKTTTLAAMIDLINQNRAEHILTI
EDPIEFVHEPIKSLIHQRQLGEDTRSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVLATLHTNSAAQTID
RIIDVFPAERQMQIRVQLSNSLLAVFSQILVPRKTVRPGGSSRVMAQEIMIVTPAIANLIREGKIAQIYSFIQTGSKLGM
QTLEKALYELFKAGLISQELALSKTSRPDEVQRLIGIL

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=515438 C6N34_RS10490 WP_115539360.1 2316273..2317349(+) (pilT) [Cylindrospermopsis raciborskii Cr2010]
ATGGAATACACAATTCAAGAACTGCTGCAAAATTTAGTAGATATGGGTGGTTCAGACCTGCATCTATCTGCAAGTTTAAG
ACCCTATTTTCGCATTGGTGGAAAACTGACTCCTATAGGTAATGAGATATTAACACCAAATGAATGTGAAAGACTGATTT
TTAGTATGTTGAATAATGACCAGCGTCACAGGTTAGAAAAAACCTGGGAGTTAGATTGTTCTTATGAAATAAAAGCTCTA
GCACGTTTTCGAGTTAACGTCTATAAAGAAAGGGGCAATTATGCAGCTTGTTTACGAGTTTTAAGTTATAAAATTCCCAA
TTTTCAAGAGTTAGGTTTGCCCAATATTGTTCGAGAAATAGCAGAAAAACCCAGAGGACTAGTTCTTGTTACAGGTCCCA
CCGGTTCTGGTAAAACCACTACCCTAGCTGCAATGATTGATTTAATCAACCAAAATCGAGCTGAACATATTCTAACCATA
GAAGACCCAATAGAATTTGTGCATGAGCCAATTAAAAGTTTAATCCACCAACGGCAATTAGGTGAAGATACACGGAGTTT
TGCCAATGCTTTAAAAGCAGCTTTACGAGAAGATCCAGATATTATTCTCGTGGGTGAAATGCGGGACCTAGAAACGATTT
CTTTAGCTATTTCCGCTGCGGAAACAGGTCATTTGGTCTTAGCCACATTACATACTAATTCAGCAGCACAGACGATTGAT
AGAATCATTGATGTTTTTCCTGCAGAACGACAAATGCAAATTAGAGTACAATTATCTAACTCCCTATTGGCAGTTTTCAG
TCAAATCCTAGTCCCTAGAAAAACCGTTAGACCAGGTGGATCTAGTCGGGTGATGGCACAGGAAATTATGATAGTTACTC
CAGCAATTGCTAACTTAATAAGGGAAGGAAAAATAGCCCAGATATATTCCTTTATTCAAACAGGAAGTAAATTGGGAATG
CAAACCTTAGAAAAAGCCCTATATGAGCTATTTAAAGCTGGTCTTATTTCTCAAGAACTAGCACTTTCTAAGACATCTAG
ACCGGATGAAGTGCAACGGTTAATTGGTATTTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(94-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

54.493

96.369

0.525
  pilT Legionella pneumophila strain Lp02

54.493

96.369

0.525
  pilT Legionella pneumophila strain ERS1305867

54.493

96.369

0.525
  pilT Pseudomonas aeruginosa PAK

53.333

96.369

0.514
  pilT Acinetobacter baumannii strain A118

53.043

96.369

0.511
  pilT Acinetobacter baumannii D1279779

53.043

96.369

0.511
  pilT Pseudomonas stutzeri DSM 10701

53.043

96.369

0.511
  pilT Acinetobacter nosocomialis M2

53.043

96.369

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.143

97.765

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.059

94.972

0.494
  pilT Vibrio cholerae strain A1552

52.059

94.972

0.494
  pilT Neisseria meningitidis 8013

51.335

94.134

0.483
  pilT Neisseria gonorrhoeae MS11

51.335

94.134

0.483
  pilU Acinetobacter baylyi ADP1

40.688

97.486

0.397
  pilU Pseudomonas stutzeri DSM 10701

40

99.162

0.397
  pilU Vibrio cholerae strain A1552

37.037

98.045

0.363