Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6G49_RS08205 Genome accession   NZ_CP065693
Coordinates   1632411..1633391 (+) Length   326 a.a.
NCBI ID   WP_059347700.1    Uniprot ID   A0A376FEY6
Organism   Enterobacter asburiae strain FDAARGOS_892     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1627411..1638391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G49_RS08165 (I6G49_08165) - 1627495..1627821 (+) 327 WP_023333354.1 YggL family protein -
  I6G49_RS08170 (I6G49_08170) - 1627878..1628594 (+) 717 WP_054830374.1 DUF2884 domain-containing protein -
  I6G49_RS08175 (I6G49_08175) - 1628677..1629015 (-) 339 WP_059347694.1 endonuclease domain-containing protein -
  I6G49_RS08180 (I6G49_08180) hemW 1629079..1630221 (-) 1143 WP_059347696.1 radical SAM family heme chaperone HemW -
  I6G49_RS08185 (I6G49_08185) - 1630214..1630807 (-) 594 WP_059347697.1 XTP/dITP diphosphatase -
  I6G49_RS08190 (I6G49_08190) yggU 1630811..1631107 (-) 297 WP_023333349.1 DUF167 family protein YggU -
  I6G49_RS08195 (I6G49_08195) - 1631104..1631670 (-) 567 WP_010435467.1 YggT family protein -
  I6G49_RS08200 (I6G49_08200) - 1631692..1632393 (-) 702 WP_059347699.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6G49_RS08205 (I6G49_08205) pilT 1632411..1633391 (+) 981 WP_059347700.1 type IV pilus twitching motility protein PilT Machinery gene
  I6G49_RS08210 (I6G49_08210) ruvX 1633402..1633818 (-) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  I6G49_RS08215 (I6G49_08215) - 1633818..1634378 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  I6G49_RS08220 (I6G49_08220) gshB 1634466..1635413 (-) 948 WP_029739222.1 glutathione synthase -
  I6G49_RS08225 (I6G49_08225) rsmE 1635433..1636164 (-) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  I6G49_RS08230 (I6G49_08230) endA 1636216..1636923 (-) 708 WP_023333343.1 deoxyribonuclease I -
  I6G49_RS08235 (I6G49_08235) - 1637018..1637515 (-) 498 WP_059347701.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35563.57 Da        Isoelectric Point: 6.6967

>NTDB_id=513048 I6G49_RS08205 WP_059347700.1 1632411..1633391(+) (pilT) [Enterobacter asburiae strain FDAARGOS_892]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGDQRLRG
SAFKQMRGVSLTLRLLPRTCPQLSSLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=513048 I6G49_RS08205 WP_059347700.1 1632411..1633391(+) (pilT) [Enterobacter asburiae strain FDAARGOS_892]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCCGCCTCCCGACGTTGGGGCGTTATTAAAAGCATGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTGACGGGAGACCAGCGGCTGCGCGGC
AGTGCCTTTAAGCAGATGAGAGGTGTCTCTTTGACGCTGCGGCTGTTGCCGCGTACCTGCCCGCAACTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTGTCCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CGGCGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGCGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A376FEY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.789

98.773

0.433
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362