Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6G66_RS17580 Genome accession   NZ_CP065668
Coordinates   3728289..3729332 (+) Length   347 a.a.
NCBI ID   WP_183020258.1    Uniprot ID   -
Organism   Delftia acidovorans strain FDAARGOS_909     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3723289..3734332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G66_RS17560 (I6G66_17555) - 3723583..3725178 (-) 1596 WP_197953760.1 AMP-binding protein -
  I6G66_RS17565 (I6G66_17560) - 3725479..3726201 (+) 723 WP_197953761.1 TetR/AcrR family transcriptional regulator -
  I6G66_RS17570 (I6G66_17565) - 3726228..3727496 (+) 1269 WP_197953762.1 branched-chain amino acid ABC transporter substrate-binding protein -
  I6G66_RS17575 (I6G66_17570) - 3727526..3728251 (-) 726 WP_197953765.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6G66_RS17580 (I6G66_17575) pilT 3728289..3729332 (+) 1044 WP_183020258.1 type IV pilus twitching motility protein PilT Machinery gene
  I6G66_RS17585 (I6G66_17580) - 3729364..3729996 (+) 633 WP_183020257.1 cyclic nucleotide-binding domain-containing protein -
  I6G66_RS17590 (I6G66_17585) pilU 3730118..3731254 (+) 1137 WP_012202997.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6G66_RS17595 (I6G66_17590) - 3731380..3732291 (+) 912 WP_012202996.1 NAD(P)-dependent oxidoreductase -
  I6G66_RS17600 (I6G66_17595) - 3732515..3733246 (-) 732 WP_183017365.1 BON domain-containing protein -
  I6G66_RS17605 (I6G66_17600) - 3733243..3733851 (-) 609 WP_012202994.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38351.10 Da        Isoelectric Point: 6.8353

>NTDB_id=512816 I6G66_RS17580 WP_183020258.1 3728289..3729332(+) (pilT) [Delftia acidovorans strain FDAARGOS_909]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMTDSQRKAYEEFLEVDFSFEIEGLAR
FRVNAFNQYRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAGMVNHLNETEYGHILTVED
PIEFVHDSKKCLINQREVGPMTQSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRIAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNLISPAEARAKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=512816 I6G66_RS17580 WP_183020258.1 3728289..3729332(+) (pilT) [Delftia acidovorans strain FDAARGOS_909]
GTGGACATCACCCAATTGCTGGCTTTCAGCGTCAAGAACAAGGCCTCCGACCTGCACCTGTCCGCGGGGCTGCCGCCGAT
GATCCGCGTGCACGGCGACGTGCGCCGCATCAATGTCGATCCGCTGGACCACAAGACCGTCCACGCCATGGTCTACGACA
TCATGACCGACTCCCAGCGCAAGGCCTACGAGGAGTTCCTTGAGGTGGACTTCTCGTTCGAGATCGAGGGCCTGGCGCGC
TTCCGGGTCAACGCCTTCAACCAGTACCGGGGTGCGGCCGCCGTCTTCCGTACCATTCCCAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCGCGCATCTTCGCCGACCTGGCGCTGAAGCCTCGCGGCCTGGTGCTGGTGACGGGGCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGGCATGGTCAACCACCTCAACGAGACCGAGTACGGGCACATCCTGACGGTGGAGGAC
CCCATCGAATTCGTGCACGACTCCAAGAAGTGCCTGATCAACCAGCGCGAGGTCGGCCCGATGACGCAGTCGTTCTCGGC
CGCCCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCCATCCTGGTGGGCGAAATGCGTGACCTGGAAACCATCCGCCTGG
CCATGACTGCGGCCGAGACGGGCCACCTGGTCTTCGGCACCCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTCTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCATTGCCGCCCACGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTGGCCCAGATGTACTCCACCATCCAGACGAGTCAGAATGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTGCGGCGCAACCTGATCAGCCCCGCGGAAGCCCGCGCCAAGGCCAAGATACCGGAGAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

75.942

99.424

0.755
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Legionella pneumophila strain Lp02

73.156

97.695

0.715
  pilT Legionella pneumophila strain ERS1305867

73.156

97.695

0.715
  pilT Neisseria meningitidis 8013

67.536

99.424

0.671
  pilT Neisseria gonorrhoeae MS11

67.246

99.424

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.985

96.542

0.415
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

41.194

96.542

0.398