Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6G82_RS12695 Genome accession   NZ_CP065643
Coordinates   2481560..2482606 (-) Length   348 a.a.
NCBI ID   WP_053994049.1    Uniprot ID   -
Organism   Lysinibacillus macroides strain FDAARGOS_925     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2476560..2487606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G82_RS12665 (I6G82_12565) - 2476846..2477538 (-) 693 WP_053994055.1 hypothetical protein -
  I6G82_RS12670 (I6G82_12570) - 2477535..2478128 (-) 594 WP_053994054.1 PilN domain-containing protein -
  I6G82_RS12675 (I6G82_12575) pilM 2478128..2479102 (-) 975 WP_053994053.1 type IV pilus biogenesis protein PilM -
  I6G82_RS12680 (I6G82_12580) - 2479215..2479691 (-) 477 WP_053994052.1 type II secretion system protein -
  I6G82_RS12685 (I6G82_12585) - 2479752..2480228 (-) 477 WP_053994051.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  I6G82_RS12690 (I6G82_12590) - 2480358..2481563 (-) 1206 WP_053994050.1 type II secretion system F family protein -
  I6G82_RS12695 (I6G82_12595) pilT 2481560..2482606 (-) 1047 WP_053994049.1 type IV pilus twitching motility protein PilT Machinery gene
  I6G82_RS12700 (I6G82_12600) - 2482618..2484288 (-) 1671 WP_200908517.1 GspE/PulE family protein -
  I6G82_RS12705 (I6G82_12605) - 2484307..2485638 (-) 1332 WP_053994048.1 G5 domain-containing protein -
  I6G82_RS12710 (I6G82_12610) - 2486042..2486512 (+) 471 WP_053994047.1 type IV pilus modification PilV family protein -
  I6G82_RS12715 (I6G82_12615) - 2486518..2486958 (+) 441 WP_053994046.1 PilW family protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38761.54 Da        Isoelectric Point: 7.1207

>NTDB_id=512478 I6G82_RS12695 WP_053994049.1 2481560..2482606(-) (pilT) [Lysinibacillus macroides strain FDAARGOS_925]
MTAYSIVELLTYAFEEKASDLHVTKGIPPVYRINGQLQQYGTVPLESEDLKEMIKVILPVHKQQDFEEKGETDFNFSLPG
KCRFRVNAYHQRNEVTIAARLIEAKIPSIEALNMPQVLYRLAEKPQGLILVTGPTGSGKSTTLAAMIDYINETRSKHIIT
LEDPIEYLHTHKKSVINQREVGVDTKSFANGLRASLRQDPDIILVGEMRDYETISTAITAAETGHLVMATLHTSSAPTTI
DRIIDVFPPHQQGQIRVQLANVLQGIVSQRLFVRKDVKGRVAATEILVQAPAISNLIRNEKIHQIPSVMQTSKALGMHTL
ESSMQHLIATGKISLEDARPYMNAGDYS

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=512478 I6G82_RS12695 WP_053994049.1 2481560..2482606(-) (pilT) [Lysinibacillus macroides strain FDAARGOS_925]
ATGACCGCGTATTCAATTGTTGAGTTATTAACATATGCATTTGAGGAAAAAGCATCAGACTTACATGTTACAAAGGGTAT
ACCACCAGTATACCGTATCAATGGGCAGCTACAGCAATATGGCACAGTGCCACTAGAATCAGAAGATTTAAAGGAAATGA
TCAAGGTTATTCTACCTGTGCATAAACAACAGGATTTTGAAGAAAAGGGAGAGACGGACTTTAACTTTTCATTGCCTGGG
AAATGTCGATTCCGTGTTAATGCCTATCATCAGCGCAATGAAGTCACAATAGCCGCTCGTTTAATCGAAGCAAAAATCCC
TTCTATTGAGGCACTCAATATGCCGCAGGTGCTATACCGACTTGCGGAAAAGCCACAGGGACTTATTTTAGTAACAGGCC
CTACTGGCTCTGGTAAATCTACAACACTAGCAGCCATGATTGATTATATTAATGAAACAAGATCTAAACATATTATTACC
TTAGAGGACCCGATTGAGTACTTGCATACCCATAAAAAGTCAGTGATCAATCAGCGTGAGGTTGGTGTCGACACCAAGAG
CTTCGCCAACGGCTTACGTGCCTCCTTGCGTCAAGACCCCGATATTATTTTAGTAGGGGAAATGCGAGACTATGAAACAA
TCTCAACAGCGATTACAGCTGCTGAAACAGGACATTTAGTCATGGCGACGCTTCATACAAGTAGTGCGCCAACGACAATT
GACCGTATTATTGATGTCTTTCCGCCACACCAGCAAGGGCAAATTCGTGTACAGCTTGCCAATGTACTACAGGGCATTGT
ATCCCAGCGCTTATTTGTGCGTAAGGATGTCAAAGGGCGTGTTGCGGCAACAGAAATTTTAGTGCAGGCGCCTGCGATTT
CTAATTTAATTCGCAATGAAAAAATCCATCAAATTCCAAGTGTAATGCAAACAAGTAAAGCATTGGGTATGCACACCTTA
GAGTCCTCCATGCAGCATTTAATTGCCACAGGAAAAATTTCATTAGAGGATGCACGACCCTATATGAATGCTGGTGATTA
TAGTTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.599

93.966

0.494
  pilT Pseudomonas aeruginosa PAK

51.198

95.977

0.491
  pilT Acinetobacter baumannii strain A118

50.599

95.977

0.486
  pilT Acinetobacter baumannii D1279779

50.599

95.977

0.486
  pilT Acinetobacter nosocomialis M2

50.299

95.977

0.483
  pilT Pseudomonas stutzeri DSM 10701

49.701

95.977

0.477
  pilT Acinetobacter baylyi ADP1

49.701

95.977

0.477
  pilT Legionella pneumophila strain ERS1305867

50.152

94.54

0.474
  pilT Legionella pneumophila strain Lp02

50.152

94.54

0.474
  pilT Neisseria meningitidis 8013

49.401

95.977

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.401

95.977

0.474
  pilT Vibrio cholerae strain A1552

49.401

95.977

0.474
  pilT Neisseria gonorrhoeae MS11

49.102

95.977

0.471
  pilU Pseudomonas stutzeri DSM 10701

42.9

95.115

0.408
  pilU Vibrio cholerae strain A1552

42.547

92.529

0.394
  pilU Acinetobacter baylyi ADP1

39.941

97.126

0.388