Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6G88_RS11440 Genome accession   NZ_CP065632
Coordinates   2508210..2509262 (+) Length   350 a.a.
NCBI ID   WP_197926031.1    Uniprot ID   -
Organism   Clostridium perfringens strain FDAARGOS_931     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2503210..2514262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G88_RS11420 (I6G88_11415) - 2503630..2504859 (+) 1230 WP_003459619.1 U32 family peptidase -
  I6G88_RS11425 (I6G88_11420) udk 2504859..2505485 (+) 627 WP_003459577.1 uridine kinase -
  I6G88_RS11430 (I6G88_11425) - 2505598..2507265 (+) 1668 WP_197926030.1 penicillin-binding transpeptidase domain-containing protein -
  I6G88_RS11435 (I6G88_11430) sigK 2507328..2508029 (+) 702 WP_003451260.1 RNA polymerase sporulation sigma factor SigK -
  I6G88_RS11440 (I6G88_11435) pilT 2508210..2509262 (+) 1053 WP_197926031.1 type IV pilus twitching motility protein PilT Machinery gene
  I6G88_RS11445 (I6G88_11440) - 2509340..2510578 (+) 1239 WP_003481817.1 cell division FtsA domain-containing protein -
  I6G88_RS11450 (I6G88_11445) ftsZ 2510592..2511737 (+) 1146 WP_197926032.1 cell division protein FtsZ -
  I6G88_RS11455 (I6G88_11450) spoIIGA 2512142..2512948 (+) 807 WP_003458523.1 sigma-E processing peptidase SpoIIGA -
  I6G88_RS11460 (I6G88_11455) sigE 2512962..2513669 (+) 708 WP_003449468.1 RNA polymerase sporulation sigma factor SigE -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39056.76 Da        Isoelectric Point: 5.4480

>NTDB_id=512339 I6G88_RS11440 WP_197926031.1 2508210..2509262(+) (pilT) [Clostridium perfringens strain FDAARGOS_931]
MQSLAELLELTVKEGASDLHLTVGISPIIKVNGKLVRLEHEILRPEDTEAYAREILQDAYEKYDAIGEYDTSYSIHGKGR
FRVNIYKQRNSTALAIRVISLDMPTLDSLGYPETLKDICNLKRGLVLVTGPTGSGKSTTLAALINEINNNRESHIITIED
PIEFLHKHNKSIVNQREIGKDTLSYERALKAALREDPDVILIGEMRDLETISTAITAAETGHLVFSTLHTIGAAKTIDRI
VDVFPPHQQEQIKIQLASVLQIIISQQLVETVDGDRNAALEIMVATPAIKNLIREGKTHQIESSIQTGSKYGMRTMDMEL
ANLYREGIITQETAMNSAIDREILSRLLMY

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=512339 I6G88_RS11440 WP_197926031.1 2508210..2509262(+) (pilT) [Clostridium perfringens strain FDAARGOS_931]
ATGCAAAGTTTAGCAGAACTTTTAGAGTTAACAGTTAAAGAAGGAGCATCTGATTTGCATTTAACAGTAGGTATTTCTCC
TATAATAAAAGTTAATGGAAAATTAGTAAGGTTAGAACATGAAATATTACGCCCTGAAGATACTGAAGCGTATGCTAGAG
AGATATTACAGGATGCATATGAAAAGTATGATGCTATTGGAGAATACGATACATCATATTCAATACATGGTAAAGGTAGA
TTTAGAGTTAATATATATAAACAAAGAAATAGTACAGCTTTAGCTATAAGAGTTATATCTTTAGATATGCCAACTTTAGA
TAGTTTAGGATATCCTGAGACTTTAAAGGATATATGTAATCTTAAAAGAGGACTTGTATTAGTTACAGGACCTACTGGAA
GTGGTAAAAGTACAACTTTAGCAGCTTTAATCAATGAAATAAATAATAATAGAGAATCTCATATAATAACAATAGAAGAT
CCTATAGAGTTTCTTCATAAACATAATAAATCTATTGTAAACCAAAGAGAGATAGGCAAGGACACTCTAAGTTATGAAAG
AGCCTTAAAAGCAGCTCTTAGAGAGGATCCTGATGTTATTTTAATTGGAGAAATGAGAGATTTAGAGACTATTTCAACAG
CTATAACAGCGGCAGAGACTGGACACTTAGTATTTTCAACTCTTCATACTATAGGAGCTGCTAAGACTATAGATAGAATA
GTAGATGTTTTTCCACCACATCAGCAGGAGCAAATAAAAATACAGTTAGCTTCTGTTTTACAAATAATTATATCACAACA
ATTAGTGGAAACCGTTGATGGAGATAGAAATGCTGCATTAGAAATAATGGTTGCTACACCAGCTATAAAAAATTTAATAA
GAGAAGGAAAAACTCATCAAATAGAATCTTCTATCCAAACTGGAAGTAAATATGGAATGAGAACGATGGACATGGAACTT
GCTAATCTGTATAGAGAAGGTATAATAACACAAGAAACGGCAATGAATTCTGCTATAGATAGGGAGATTTTAAGTCGATT
ATTGATGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.711

98.857

0.491
  pilT Neisseria meningitidis 8013

49.709

98.286

0.489
  pilT Neisseria gonorrhoeae MS11

49.709

98.286

0.489
  pilT Pseudomonas aeruginosa PAK

50.595

96

0.486
  pilT Pseudomonas stutzeri DSM 10701

50.298

96

0.483
  pilT Acinetobacter baylyi ADP1

50.904

94.857

0.483
  pilT Legionella pneumophila strain Lp02

49.123

97.714

0.48
  pilT Legionella pneumophila strain ERS1305867

49.123

97.714

0.48
  pilT Acinetobacter baumannii D1279779

49.702

96

0.477
  pilT Acinetobacter nosocomialis M2

49.702

96

0.477
  pilT Acinetobacter baumannii strain A118

49.702

96

0.477
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

93.429

0.474
  pilT Vibrio cholerae strain A1552

50.765

93.429

0.474
  pilU Pseudomonas stutzeri DSM 10701

43.976

94.857

0.417
  pilU Vibrio cholerae strain A1552

40.653

96.286

0.391
  pilU Acinetobacter baylyi ADP1

39.823

96.857

0.386