Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ITG08_RS03650 Genome accession   NZ_CP065206
Coordinates   816675..817784 (-) Length   369 a.a.
NCBI ID   WP_010435664.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain ED653     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 811675..822784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG08_RS03615 (ITG08_03605) - 812043..812543 (+) 501 WP_010435678.1 SprT family zinc-dependent metalloprotease -
  ITG08_RS03620 (ITG08_03610) - 812640..813371 (+) 732 WP_010435675.1 endonuclease -
  ITG08_RS03625 (ITG08_03615) rsmE 813516..814247 (+) 732 WP_010435671.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ITG08_RS03630 (ITG08_03620) gshB 814257..815207 (+) 951 WP_004735482.1 glutathione synthase -
  ITG08_RS03635 (ITG08_03625) - 815324..815887 (+) 564 WP_016768176.1 YqgE/AlgH family protein -
  ITG08_RS03640 (ITG08_03630) ruvX 815936..816358 (+) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  ITG08_RS03645 (ITG08_03635) - 816457..816618 (+) 162 WP_016785941.1 hypothetical protein -
  ITG08_RS03650 (ITG08_03640) pilU 816675..817784 (-) 1110 WP_010435664.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ITG08_RS03655 (ITG08_03645) pilT 817799..818836 (-) 1038 WP_010435660.1 type IV pilus twitching motility protein PilT Machinery gene
  ITG08_RS03660 (ITG08_03650) - 818863..819567 (+) 705 WP_010435657.1 YggS family pyridoxal phosphate-dependent enzyme -
  ITG08_RS03665 (ITG08_03655) proC 819680..820498 (+) 819 WP_010435654.1 pyrroline-5-carboxylate reductase -
  ITG08_RS03670 (ITG08_03660) - 820575..821132 (+) 558 WP_010435653.1 YggT family protein -
  ITG08_RS03675 (ITG08_03665) yggU 821162..821452 (+) 291 WP_010435651.1 DUF167 family protein YggU -
  ITG08_RS03680 (ITG08_03670) - 821483..821914 (+) 432 WP_016796311.1 DUF4426 domain-containing protein -
  ITG08_RS03685 (ITG08_03675) - 821963..822562 (+) 600 WP_010435646.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41308.30 Da        Isoelectric Point: 6.0672

>NTDB_id=508005 ITG08_RS03650 WP_010435664.1 816675..817784(-) (pilU) [Vibrio cyclitrophicus strain ED653]
MELNQILEGMLSQKASDLYITVDAPVLFRVDGELRPQGEKLNAAQVALLLDAMMEQDRRDEYKQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKDQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKSTMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTKRGDDDYGVGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=508005 ITG08_RS03650 WP_010435664.1 816675..817784(-) (pilU) [Vibrio cyclitrophicus strain ED653]
ATGGAATTGAATCAAATTCTTGAAGGTATGCTTTCACAAAAAGCGTCGGATCTTTACATCACGGTAGATGCACCGGTTCT
GTTTCGTGTTGATGGTGAATTGCGACCTCAGGGAGAGAAACTGAATGCGGCTCAGGTTGCGCTGTTACTGGATGCGATGA
TGGAGCAAGACAGGCGCGACGAGTACAAACAAACGCGTGAGGCTAACTTTGCTATTGTGCGTGATTTTGGTCGTTTTCGT
GTTAGCGCATTCTTTCAGCGAGAGCTACCAGGAGCGGTAATTCGACGTATCGAGACTAACATCCCAACCTTCGAACAATT
AAAGCTTCCAGACGTACTACAAGACCTTTCAATCGCAAAACGCGGACTTGTGTTGGTGGTTGGGGCTACAGGTTCTGGTA
AATCAACCTCAATGGCTGCGATGACGGGGTATCGCAATACCAACCGTTCGGGGCATATCTTGACGGTTGAAGACCCGATT
GAGTTCGTGCACGAACATAAAAAGTGCATTGTGACTCAGCGAGAAGTTGGGCTCGATACCGAGAGCTATGAAGTCGCGCT
TAAGAACTCGTTACGCCAAGCCCCTGACATGATCTTGATTGGTGAAATCCGTACCCGTGAAACCATGGAGTACGCGATGA
CGTTTGCTGAAACCGGTCACCTTTGCATGGCGACTTTACATGCCAACAATGCCAACCAAGCGCTAGAGCGTATTCTTCAC
TTGGTGCCGAAAGAGCAAAAAGATCAGTTCTTATTTGATTTGTCGATGAATCTACGTGGTGTCGTTGCTCAGCAATTAAT
CCGAGATAAGAATGGTAGTGGTCGCCATGGTGTGTTCGAAATCCTACTCAACAGCCCACGGGTGTCTGATTTGATTCGTA
GAGGTGAGTTACATGAACTCAAGTCGACAATGGCTAAATCAAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GACTTAGTCGTTGCAGGCAAGATCAGTGAAGAGGATGCGTTCCATAGTGCCGATTCCGCTAATGATTTGCGCTTGATGCT
CAAGACTAAACGTGGTGATGACGATTACGGAGTGGGAGCCTTAGCGGGAGTTAAAATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.967

99.729

0.837
  pilU Pseudomonas stutzeri DSM 10701

58.857

94.851

0.558
  pilU Acinetobacter baylyi ADP1

53.846

95.122

0.512
  pilT Acinetobacter baumannii strain A118

42.398

92.683

0.393
  pilT Acinetobacter baumannii D1279779

42.398

92.683

0.393
  pilT Acinetobacter nosocomialis M2

42.398

92.683

0.393
  pilT Legionella pneumophila strain ERS1305867

42.478

91.87

0.39
  pilT Legionella pneumophila strain Lp02

42.478

91.87

0.39
  pilT Pseudomonas aeruginosa PAK

41.888

91.87

0.385
  pilT Acinetobacter baylyi ADP1

42.47

89.973

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas stutzeri DSM 10701

41.369

91.057

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.515

0.371
  pilT Vibrio cholerae strain A1552

41.018

90.515

0.371
  pilT Neisseria gonorrhoeae MS11

38.551

93.496

0.36
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36