Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I2D83_RS04445 Genome accession   NZ_CP065044
Coordinates   997726..998742 (-) Length   338 a.a.
NCBI ID   WP_205947616.1    Uniprot ID   -
Organism   Pectobacterium aroidearum strain L6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 992726..1003742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I2D83_RS04430 (I2D83_04435) nsrR 993090..993515 (-) 426 WP_015841587.1 nitric oxide-sensing transcriptional repressor NsrR -
  I2D83_RS04435 (I2D83_04440) - 993833..995404 (+) 1572 WP_180778637.1 methyl-accepting chemotaxis protein -
  I2D83_RS04440 (I2D83_04445) - 995931..997757 (+) 1827 WP_205947615.1 diguanylate cyclase -
  I2D83_RS04445 (I2D83_04450) pilT 997726..998742 (-) 1017 WP_205947616.1 type IV pilus twitching motility protein PilT Machinery gene
  I2D83_RS04450 (I2D83_04455) - 998764..999477 (+) 714 WP_205947617.1 YggS family pyridoxal phosphate-dependent enzyme -
  I2D83_RS04455 (I2D83_04460) proC 999568..1000389 (+) 822 WP_205947618.1 pyrroline-5-carboxylate reductase -
  I2D83_RS04460 (I2D83_04465) - 1000438..1000992 (+) 555 WP_010295003.1 YggT family protein -
  I2D83_RS04465 (I2D83_04470) yggU 1000989..1001279 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  I2D83_RS04470 (I2D83_04475) - 1001297..1001890 (+) 594 WP_014916538.1 XTP/dITP diphosphatase -
  I2D83_RS04475 (I2D83_04480) hemW 1001883..1003025 (+) 1143 WP_205947619.1 radical SAM family heme chaperone HemW -
  I2D83_RS04480 (I2D83_04485) - 1003253..1003513 (+) 261 WP_015841595.1 type II toxin-antitoxin system ParD family antitoxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36897.17 Da        Isoelectric Point: 6.8717

>NTDB_id=506169 I2D83_RS04445 WP_205947616.1 997726..998742(-) (pilT) [Pectobacterium aroidearum strain L6]
MELDEWIARSVKHNASDLHLCSGHPPVLRVDGRLQSENTLPRLTSEQVAQWCDAWLEPAQREQLRQFGQIDGALMLPDGL
RLRVNVFRQREGLSAALRIIPSVQPSLDGLHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSRDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLSTCLQAVVTQRLLPSAHGGRIALYEVLTATAAVGNLIREGKTYQLPGLIQTGTAAGMQTFEQS
YQQRCRDGLISHNCALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=506169 I2D83_RS04445 WP_205947616.1 997726..998742(-) (pilT) [Pectobacterium aroidearum strain L6]
ATGGAATTGGATGAGTGGATAGCGCGTAGTGTAAAACATAATGCCTCAGATCTGCACCTTTGTAGCGGCCATCCGCCAGT
GTTACGTGTCGATGGGCGGCTACAGTCTGAAAATACCTTACCACGTTTAACGTCAGAGCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCCGCTCAGAGAGAACAATTGCGGCAGTTTGGTCAGATAGACGGCGCGTTAATGCTGCCTGATGGGCTG
CGTCTGCGGGTAAATGTGTTTCGTCAGCGAGAAGGGCTGTCCGCGGCGCTGCGTATTATCCCGTCCGTTCAGCCGTCATT
GGACGGGCTACATGCGCCACCCGTTCTGTCGACGTTGCTGGAGAAACCGAACGGGCTGATCCTGATTACCGGAGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCAATGCTCGGGGCGTTAAATGACAGTCGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATCCATGCCAGCCGGCGCTGCCTGATTCAACAGCGAGAGATCGGTGCGCACAGCGCGTCTTTTGC
ACAGGCGCTACGGGCCGCCTTGCGGGAAGATCCCGATGTGATTCTGCTGGGGGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGTCATCTGGTGCTATCCACGCTGCATACGCGCAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCTGGTGAAGAGAAAGCCTATGTTCGCAGTCAGCTATCCACCTGTTTGCAGGCCGTGGTGACGCA
AAGGTTGTTGCCTTCTGCACACGGCGGCCGCATTGCGCTTTATGAAGTACTGACGGCGACAGCGGCAGTCGGCAACCTGA
TTCGGGAAGGGAAAACGTATCAGCTTCCCGGCCTGATTCAAACGGGAACGGCTGCGGGCATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAATTGCGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.298

99.408

0.5
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Neisseria gonorrhoeae MS11

50

99.408

0.497
  pilT Legionella pneumophila strain ERS1305867

49.552

99.112

0.491
  pilT Legionella pneumophila strain Lp02

49.552

99.112

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.552

99.112

0.491
  pilT Acinetobacter baylyi ADP1

49.112

100

0.491
  pilT Pseudomonas aeruginosa PAK

49.254

99.112

0.488
  pilT Acinetobacter nosocomialis M2

48.657

99.112

0.482
  pilT Acinetobacter baumannii D1279779

48.657

99.112

0.482
  pilT Acinetobacter baumannii strain A118

48.657

99.112

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.543

99.704

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.763

99.704

0.396
  pilU Vibrio cholerae strain A1552

39.466

99.704

0.393
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367