Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PB20LOC_RS15075 Genome accession   NZ_CP065036
Coordinates   3335807..3336823 (-) Length   338 a.a.
NCBI ID   WP_014701254.1    Uniprot ID   A0A0H3I760
Organism   Pectobacterium parmentieri strain PB20     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3330807..3341823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PB20LOC_RS15060 (PB20LOC_015060) nsrR 3331216..3331641 (-) 426 WP_005974824.1 nitric oxide-sensing transcriptional repressor NsrR -
  PB20LOC_RS15065 (PB20LOC_015065) - 3331930..3333501 (+) 1572 WP_014701252.1 methyl-accepting chemotaxis protein -
  PB20LOC_RS15070 (PB20LOC_015070) - 3334006..3335838 (+) 1833 WP_014701253.1 diguanylate cyclase -
  PB20LOC_RS15075 (PB20LOC_015075) pilT 3335807..3336823 (-) 1017 WP_014701254.1 type IV pilus twitching motility protein PilT Machinery gene
  PB20LOC_RS15080 (PB20LOC_015080) - 3336845..3337558 (+) 714 WP_014701255.1 YggS family pyridoxal phosphate-dependent enzyme -
  PB20LOC_RS15085 (PB20LOC_015085) proC 3337635..3338456 (+) 822 WP_015731141.1 pyrroline-5-carboxylate reductase -
  PB20LOC_RS15090 (PB20LOC_015090) - 3338506..3339075 (+) 570 WP_014701257.1 YggT family protein -
  PB20LOC_RS15095 (PB20LOC_015095) yggU 3339072..3339362 (+) 291 WP_103807946.1 DUF167 family protein YggU -
  PB20LOC_RS15100 (PB20LOC_015100) - 3339380..3339973 (+) 594 WP_014701259.1 XTP/dITP diphosphatase -
  PB20LOC_RS15105 (PB20LOC_015105) hemW 3339966..3341105 (+) 1140 WP_103807947.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36964.29 Da        Isoelectric Point: 6.3640

>NTDB_id=506025 PB20LOC_RS15075 WP_014701254.1 3335807..3336823(-) (pilT) [Pectobacterium parmentieri strain PB20]
MELDEWVARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPEQVAQWCDAWLEPVQREQLRQIGQVDGTLMLPDGQ
RLRVNVFHQREGLSVALRIIPFFQPSLDELHAPPVLATLLEKPNGLILITGATGSGKSTTLAAMVGALNDSSDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSAPQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVAQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLMQTGVAAGMQTFEQS
DQQRCRDGLISHSYALAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=506025 PB20LOC_RS15075 WP_014701254.1 3335807..3336823(-) (pilT) [Pectobacterium parmentieri strain PB20]
ATGGAGTTGGATGAGTGGGTGGCGCGTAGTGTAAAACATAATGCCTCTGATCTGCACCTTTGTAGCGGGCACCCTCCGGT
GTTACGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCGGAACAGGTGGCACAATGGTGTGATG
CCTGGCTCGAACCGGTTCAGAGAGAGCAGTTGCGGCAGATTGGGCAAGTGGACGGCACGTTAATGTTGCCTGATGGGCAA
CGGCTGCGGGTCAATGTGTTTCACCAGCGGGAAGGGCTGTCTGTCGCGCTGCGTATTATCCCGTTTTTTCAACCTTCATT
GGATGAATTACATGCGCCACCCGTTCTTGCGACGCTGTTGGAGAAACCAAATGGGCTGATCCTGATTACTGGTGCGACGG
GCAGCGGTAAATCTACGACGTTGGCGGCAATGGTCGGGGCGTTAAATGACAGCAGCGATCGCCATGTGATCACGTTGGAA
GATCCGATCGAATTTATTCATACCAGCCGACGCTGCCTGATCCAACAACGAGAGATCGGTGCACACAGCGCGTCTTTTGC
GCAGGCGCTACGGGCGGCATTAAGGGAAGATCCCGATGTTATTCTACTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGTTAACGGCGGCAGAAACCGGTCATCTGGTGCTGTCAACGTTACATACACGTAGTGCACCGCAGGCTGTTGATCGG
TTGATTGATGTCTTCCCCGGTGAAGAGAAAGCGTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCCGTGGTGGCGCA
AAAATTGTTGCCCGCAGCACAAGGTGGCCGAATCGCGCTGTATGAAGTGCTGACTGCCACCGCGGCAGTCAGCAACTTGA
TTCGTGAGGGGAAAACGCACCAGCTTCCCGGCCTGATGCAAACCGGAGTTGCTGCGGGAATGCAAACGTTCGAACAAAGC
GACCAGCAGCGTTGCCGGGATGGGCTTATTTCACACAGTTACGCCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3I760

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.909

97.633

0.497
  pilT Vibrio cholerae strain A1552

50.909

97.633

0.497
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Legionella pneumophila strain Lp02

48.06

99.112

0.476
  pilT Legionella pneumophila strain ERS1305867

48.06

99.112

0.476
  pilT Acinetobacter baylyi ADP1

47.633

100

0.476
  pilT Pseudomonas aeruginosa PAK

47.761

99.112

0.473
  pilT Pseudomonas stutzeri DSM 10701

47.761

99.112

0.473
  pilT Acinetobacter nosocomialis M2

47.13

97.929

0.462
  pilT Acinetobacter baumannii D1279779

47.13

97.929

0.462
  pilT Acinetobacter baumannii strain A118

47.13

97.929

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Pseudomonas stutzeri DSM 10701

40.653

99.704

0.405
  pilU Vibrio cholerae strain A1552

39.169

99.704

0.391
  pilU Acinetobacter baylyi ADP1

39.13

95.266

0.373