Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DKB71_RS31410 Genome accession   NZ_CP064949
Coordinates   6875118..6876152 (-) Length   344 a.a.
NCBI ID   WP_408599231.1    Uniprot ID   -
Organism   Pseudomonas sp. PLMAX     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6870118..6881152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKB71_RS31380 (DKB71_31025) ruvX 6870267..6870704 (+) 438 WP_408599230.1 Holliday junction resolvase RuvX -
  DKB71_RS31385 (DKB71_31030) pyrR 6870791..6871294 (+) 504 WP_274075643.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  DKB71_RS31390 (DKB71_31035) - 6871322..6872326 (+) 1005 WP_046818333.1 aspartate carbamoyltransferase catalytic subunit -
  DKB71_RS31395 (DKB71_31040) - 6872323..6873594 (+) 1272 WP_274075644.1 dihydroorotase -
  DKB71_RS31400 (DKB71_31045) - 6873688..6874119 (-) 432 WP_323627282.1 TM2 domain-containing protein -
  DKB71_RS31405 (DKB71_31050) - 6874369..6874986 (+) 618 WP_274075646.1 C40 family peptidase -
  DKB71_RS31410 (DKB71_31055) pilT 6875118..6876152 (-) 1035 WP_408599231.1 type IV pilus twitching motility protein PilT Machinery gene
  DKB71_RS31415 (DKB71_31060) - 6876209..6876895 (+) 687 WP_323606618.1 YggS family pyridoxal phosphate-dependent enzyme -
  DKB71_RS31420 (DKB71_31065) proC 6876918..6877736 (+) 819 WP_323606619.1 pyrroline-5-carboxylate reductase -
  DKB71_RS31425 (DKB71_31070) - 6877761..6878348 (+) 588 WP_274075651.1 YggT family protein -
  DKB71_RS31430 (DKB71_31075) - 6878532..6879671 (+) 1140 WP_408599232.1 homoserine O-acetyltransferase -
  DKB71_RS31435 (DKB71_31080) metW 6879679..6880299 (+) 621 WP_274075653.1 methionine biosynthesis protein MetW -
  DKB71_RS31440 (DKB71_31085) - 6880326..6880760 (+) 435 WP_408599233.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37991.50 Da        Isoelectric Point: 6.9826

>NTDB_id=505773 DKB71_RS31410 WP_408599231.1 6875118..6876152(-) (pilT) [Pseudomonas sp. PLMAX]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALEHKEVQALIYDIMNDTQRVAFEQHLETDFSFEVPGIAR
FRVNAFNQSRGAGAVFRTIPSRVRSMEELGMGEVFHKMTDAPRGLVLVTGPTGSGKSTTLAAMIDYLNSHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRVAAHEIMLGTSAIRNLIREDKVAQMYSSIQSGGSLGMQTLDMSL
KDLVTRGLVSRDQAREKARSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=505773 DKB71_RS31410 WP_408599231.1 6875118..6876152(-) (pilT) [Pseudomonas sp. PLMAX]
ATGGATATCACTGAGTTGCTGGCGTTCAGCGCCAAACAAGGCGCGTCCGACCTGCATTTGTCGGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGCGACGTGCGGCGCATCAACTTGCCGGCCCTGGAGCACAAGGAGGTGCAGGCGCTGATCTACGACA
TCATGAACGATACCCAGCGGGTGGCGTTCGAACAGCATCTGGAAACCGACTTTTCCTTTGAGGTGCCCGGCATCGCGCGC
TTCCGGGTCAATGCCTTCAATCAGAGCCGAGGCGCTGGTGCGGTGTTTCGTACGATCCCGTCCAGGGTCCGGAGCATGGA
AGAGCTGGGCATGGGCGAAGTGTTTCACAAGATGACTGATGCACCCCGCGGCCTGGTACTGGTGACCGGCCCGACGGGGT
CCGGCAAGTCCACCACTCTGGCAGCGATGATCGATTACCTGAACAGCCATCGCCATCACCACATCCTGACGATTGAAGAT
CCTATCGAGTTCGTCCACGAGTCGCGCAAATGCCTGATCAATCAGCGTGAAGTCCACCGCGATACCCGCAGTTTCGCCAC
AGCCTTGCGCTCGGCCCTGCGCGAAGACCCGGACGTGATCCTGGTGGGGGAGCTGCGTGACCTGGAGACTATCCGCCTGG
CGTTGACCGCTGCCGAGACGGGTCACCTGGTGTTCGGCACGCTGCACACGACCTCGGCGGCGAAAACCATCGACCGGATC
ATCGACGTGTTTCCCGGGGATGAGAAGTCGATGGTGCGTTCGATGCTGTCCGAATCGTTGCTGGCGGTGGTGTCCCAGAC
CTTGGTCAAGAAGATTGGCGGTGGTCGGGTGGCGGCCCACGAGATCATGCTGGGAACGTCGGCGATTCGTAACCTGATCC
GTGAAGACAAGGTGGCGCAGATGTATTCGTCGATTCAGTCGGGCGGGTCGCTGGGGATGCAGACGCTGGATATGAGTTTG
AAGGATCTGGTGACCAGGGGGTTGGTCAGTCGCGATCAGGCGCGGGAGAAGGCGCGGTCGCCGGATAATTTTTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

84.302

100

0.843
  pilT Pseudomonas stutzeri DSM 10701

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baumannii D1279779

77.326

100

0.773
  pilT Acinetobacter baumannii strain A118

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

72.674

100

0.727
  pilT Legionella pneumophila strain ERS1305867

72.674

100

0.727
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria meningitidis 8013

65.507

100

0.657
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Vibrio cholerae strain A1552

41.449

100

0.416
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401