Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Azoinq_RS05800 Genome accession   NZ_CP064782
Coordinates   1281425..1282468 (-) Length   347 a.a.
NCBI ID   WP_216131143.1    Uniprot ID   A0A975SPG7
Organism   Azospira inquinata strain Azo-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1276425..1287468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Azoinq_RS05775 (Azoinq_05770) - 1277226..1277648 (+) 423 WP_216131158.1 CopD family protein -
  Azoinq_RS05780 (Azoinq_05775) - 1277733..1278911 (+) 1179 WP_216131155.1 class I SAM-dependent RNA methyltransferase -
  Azoinq_RS05785 (Azoinq_05780) - 1278982..1279854 (+) 873 WP_216131152.1 phosphatase PAP2 family protein -
  Azoinq_RS05790 (Azoinq_05785) - 1279893..1280195 (-) 303 WP_216131149.1 hypothetical protein -
  Azoinq_RS05795 (Azoinq_05790) pilU 1280235..1281371 (-) 1137 WP_216131146.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Azoinq_RS05800 (Azoinq_05795) pilT 1281425..1282468 (-) 1044 WP_216131143.1 type IV pilus twitching motility protein PilT Machinery gene
  Azoinq_RS05805 (Azoinq_05800) - 1282586..1283308 (+) 723 WP_216131140.1 YggS family pyridoxal phosphate-dependent enzyme -
  Azoinq_RS05810 (Azoinq_05805) proC 1283305..1284123 (+) 819 WP_216131137.1 pyrroline-5-carboxylate reductase -
  Azoinq_RS05815 (Azoinq_05810) - 1284311..1284883 (+) 573 WP_216131134.1 YggT family protein -
  Azoinq_RS05820 (Azoinq_05815) - 1284883..1285179 (+) 297 WP_216131131.1 DUF167 domain-containing protein -
  Azoinq_RS05825 (Azoinq_05820) - 1285283..1285999 (-) 717 WP_216131128.1 PAS domain-containing transcriptional regulator -
  Azoinq_RS05830 (Azoinq_05825) cowN 1286141..1286428 (+) 288 WP_216131124.1 N(2)-fixation sustaining protein CowN -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38452.32 Da        Isoelectric Point: 7.1066

>NTDB_id=504722 Azoinq_RS05800 WP_216131143.1 1281425..1282468(-) (pilT) [Azospira inquinata strain Azo-3]
MDITELLAFGVKNKASDLHLSAGLPPMIRVNGDVRRINLPPMEHKDVHAMVYDIMSDSQRKHYEENLECDFSFEIPNLAR
FRVNAFVQHRGAGAVFRTIPSKVLTLEELNCPKIFKDIAEYPRGIVLVTGPTGSGKSTTLAAMVNHVNENFYGHILTVED
PIEFVHEAKKSLINQREVGPHTLSFNNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAAEKDMIRAMLSESLRAVISQTLLKTKDGNGRVAAHEIMLGTPAIRNLIRENKVAQMYSSIQTGQSYGMQTLDQC
LLDLVRRNLVAPVEAKAKAVNKDMFGA

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=504722 Azoinq_RS05800 WP_216131143.1 1281425..1282468(-) (pilT) [Azospira inquinata strain Azo-3]
ATGGATATCACCGAACTTCTCGCTTTCGGGGTCAAAAACAAGGCTTCCGACCTTCACCTTTCCGCGGGCCTGCCGCCCAT
GATCCGGGTTAACGGCGACGTGCGCCGCATCAACCTGCCCCCCATGGAGCACAAGGATGTGCATGCCATGGTGTACGACA
TCATGAGCGACTCCCAGCGTAAGCATTACGAGGAAAACCTGGAGTGCGACTTTTCCTTTGAAATCCCCAATCTGGCCCGT
TTCCGGGTCAATGCCTTTGTCCAGCATCGGGGTGCCGGGGCCGTGTTCCGGACCATTCCGTCCAAGGTCCTGACCCTGGA
AGAACTCAACTGTCCGAAAATTTTCAAAGATATTGCCGAATATCCCCGGGGCATTGTGCTGGTCACCGGTCCCACGGGCT
CGGGGAAATCCACCACCCTGGCGGCCATGGTGAATCACGTGAACGAAAACTTCTACGGCCACATCCTGACCGTGGAAGAC
CCTATCGAATTCGTGCATGAAGCCAAGAAGAGCCTGATCAACCAGCGGGAAGTGGGACCCCACACCCTGTCCTTCAACAA
CGCCCTGCGTTCCGCCCTGCGGGAAGATCCGGACGTGATTCTGGTGGGGGAATTGCGGGATTTGGAAACCATCCGGCTGG
CCATGACCGCGGCGGAAACGGGCCACTTGGTGTTCGGCACCCTGCACACTTCCTCTGCCGCCAAAACGGTGGACCGGATC
ATCGACGTGTTCCCGGCGGCGGAAAAGGACATGATTCGGGCCATGTTGTCCGAATCCCTGCGGGCGGTGATTTCCCAGAC
CCTGCTGAAAACCAAGGATGGTAACGGTCGGGTGGCGGCCCACGAAATCATGCTGGGCACCCCGGCTATCCGCAATCTGA
TCCGGGAAAACAAGGTGGCCCAGATGTATTCCTCCATCCAGACCGGCCAGTCCTACGGCATGCAGACCCTGGACCAATGC
CTCCTGGATCTGGTGCGCCGCAATCTGGTGGCCCCCGTGGAAGCCAAGGCCAAAGCGGTGAATAAGGACATGTTCGGGGC
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Pseudomonas stutzeri DSM 10701

73.913

99.424

0.735
  pilT Pseudomonas aeruginosa PAK

74.926

97.695

0.732
  pilT Legionella pneumophila strain Lp02

72.174

99.424

0.718
  pilT Legionella pneumophila strain ERS1305867

72.174

99.424

0.718
  pilT Neisseria meningitidis 8013

71.72

98.847

0.709
  pilT Neisseria gonorrhoeae MS11

71.429

98.847

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.231

97.406

0.674
  pilT Vibrio cholerae strain A1552

69.231

97.406

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

99.424

0.516
  pilU Pseudomonas stutzeri DSM 10701

42.593

93.372

0.398
  pilU Acinetobacter baylyi ADP1

41.071

96.83

0.398
  pilU Vibrio cholerae strain A1552

42.154

93.66

0.395