Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IT784_RS03730 Genome accession   NZ_CP064709
Coordinates   774692..775672 (+) Length   326 a.a.
NCBI ID   WP_001055645.1    Uniprot ID   A0A3A3J1I8
Organism   Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC 74     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 769692..780672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IT784_RS03700 (IT784_03700) - 770270..771277 (+) 1008 WP_000252191.1 DUF1202 domain-containing protein -
  IT784_RS03705 (IT784_03705) hemW 771368..772504 (-) 1137 WP_088546055.1 radical SAM family heme chaperone HemW -
  IT784_RS03710 (IT784_03710) - 772497..773090 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  IT784_RS03715 (IT784_03715) yggU 773098..773388 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  IT784_RS03720 (IT784_03720) - 773385..773951 (-) 567 WP_001094848.1 YggT family protein -
  IT784_RS03725 (IT784_03725) - 773970..774674 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  IT784_RS03730 (IT784_03730) pilT 774692..775672 (+) 981 WP_001055645.1 type IV pilus twitching motility protein PilT Machinery gene
  IT784_RS03735 (IT784_03735) - 775804..776505 (+) 702 WP_195700873.1 IclR family transcriptional regulator -
  IT784_RS03740 (IT784_03740) ruvX 776537..776953 (-) 417 WP_088546057.1 Holliday junction resolvase RuvX -
  IT784_RS03745 (IT784_03745) - 776953..777516 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  IT784_RS03750 (IT784_03750) gshB 777732..778679 (-) 948 WP_061377598.1 glutathione synthase -
  IT784_RS03755 (IT784_03755) rsmE 778699..779430 (-) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IT784_RS03760 (IT784_03760) endA 779507..780214 (-) 708 WP_069721783.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36165.35 Da        Isoelectric Point: 7.5116

>NTDB_id=504473 IT784_RS03730 WP_001055645.1 774692..775672(+) (pilT) [Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC 74]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGYLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPIRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=504473 IT784_RS03730 WP_001055645.1 774692..775672(+) (pilT) [Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC 74]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAGT
CCACCACGCTGGCGGCGATGGTGGGATATCTTAATCAACATGCCGATAAACATATCCTGACGCTGGAAGATCCTATTGAA
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGATACGCAGTCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A3J1I8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.26

100

0.454
  pilT Acinetobacter baylyi ADP1

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Pseudomonas stutzeri DSM 10701

36.254

100

0.368
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365