Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IUJ44_RS09640 Genome accession   NZ_CP064375
Coordinates   2051262..2052299 (-) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain PartI-Abaumannii-RM8376 isolate ATCC isolate     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2014657..2081809 2051262..2052299 within 0


Gene organization within MGE regions


Location: 2014657..2081809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IUJ44_RS09460 (IUJ44_09450) - 2015058..2015618 (-) 561 WP_001048354.1 hypothetical protein -
  IUJ44_RS09465 (IUJ44_09455) - 2015709..2016569 (-) 861 WP_000838088.1 pirin family protein -
  IUJ44_RS09470 (IUJ44_09460) hemJ 2016740..2017192 (-) 453 WP_000339889.1 protoporphyrinogen oxidase HemJ -
  IUJ44_RS09475 (IUJ44_09465) - 2017211..2018440 (-) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  IUJ44_RS09480 (IUJ44_09470) - 2018737..2020062 (+) 1326 WP_000160010.1 EcsC family protein -
  IUJ44_RS09485 (IUJ44_09475) rpsL 2020263..2020637 (+) 375 WP_000246374.1 30S ribosomal protein S12 -
  IUJ44_RS09490 (IUJ44_09480) rpsG 2020797..2021267 (+) 471 WP_001138055.1 30S ribosomal protein S7 -
  IUJ44_RS09495 (IUJ44_09485) fusA 2021446..2023584 (+) 2139 WP_000113824.1 elongation factor G -
  IUJ44_RS09500 (IUJ44_09490) tuf 2023679..2024869 (+) 1191 WP_001029610.1 elongation factor Tu -
  IUJ44_RS09505 (IUJ44_09495) - 2025073..2025954 (+) 882 WP_000992301.1 metal-dependent hydrolase -
  IUJ44_RS09510 (IUJ44_09500) - 2026191..2027066 (+) 876 WP_000992291.1 metal-dependent hydrolase -
  IUJ44_RS09515 (IUJ44_09505) rimI 2027174..2027629 (+) 456 WP_000621546.1 ribosomal protein S18-alanine N-acetyltransferase -
  IUJ44_RS09520 (IUJ44_09510) - 2027674..2028489 (-) 816 WP_000844343.1 arginyltransferase -
  IUJ44_RS09525 (IUJ44_09515) aat 2028508..2029239 (-) 732 WP_000854789.1 leucyl/phenylalanyl-tRNA--protein transferase -
  IUJ44_RS09530 (IUJ44_09520) trxB 2029360..2030307 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  IUJ44_RS09535 (IUJ44_09525) - 2030577..2033609 (+) 3033 WP_000127835.1 DNA translocase FtsK -
  IUJ44_RS09540 (IUJ44_09530) rhtC 2033758..2034378 (+) 621 WP_000959259.1 threonine export protein RhtC -
  IUJ44_RS09545 (IUJ44_09535) - 2034421..2034708 (-) 288 WP_001218560.1 PA4642 family protein -
  IUJ44_RS09550 (IUJ44_09540) minE 2034792..2035064 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  IUJ44_RS09555 (IUJ44_09545) minD 2035067..2035879 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  IUJ44_RS09560 (IUJ44_09550) minC 2035950..2036672 (-) 723 WP_000763677.1 septum site-determining protein MinC -
  IUJ44_RS09565 (IUJ44_09555) - 2036794..2037840 (-) 1047 WP_001181637.1 hypothetical protein -
  IUJ44_RS09570 (IUJ44_09560) - 2037857..2038783 (-) 927 WP_000100975.1 acyltransferase -
  IUJ44_RS09575 (IUJ44_09565) - 2039028..2039681 (+) 654 WP_001202415.1 OmpA family protein -
  IUJ44_RS09580 (IUJ44_09570) rep 2039875..2041914 (+) 2040 WP_000093035.1 DNA helicase Rep -
  IUJ44_RS09585 (IUJ44_09575) dut 2041940..2042392 (+) 453 WP_000868152.1 dUTP diphosphatase -
  IUJ44_RS09590 (IUJ44_09580) - 2042592..2044010 (+) 1419 WP_001102833.1 phosphomannomutase/phosphoglucomutase -
  IUJ44_RS09595 (IUJ44_09585) argB 2044025..2044933 (+) 909 WP_001135419.1 acetylglutamate kinase -
  IUJ44_RS09600 (IUJ44_09590) - 2045054..2045836 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  IUJ44_RS09605 (IUJ44_09595) - 2045945..2046793 (+) 849 WP_000336554.1 class II glutamine amidotransferase -
  IUJ44_RS09610 (IUJ44_09600) - 2047046..2047501 (+) 456 WP_000782976.1 bacteriohemerythrin -
  IUJ44_RS09615 (IUJ44_09605) - 2047654..2048721 (+) 1068 WP_005144332.1 hypothetical protein -
  IUJ44_RS09620 (IUJ44_09610) - 2048721..2049044 (+) 324 WP_000442584.1 RnfH family protein -
  IUJ44_RS09625 (IUJ44_09615) bamE 2049099..2049497 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  IUJ44_RS09630 (IUJ44_09620) fur 2049609..2050046 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  IUJ44_RS09635 (IUJ44_09625) pilU 2050116..2051234 (-) 1119 WP_000347036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IUJ44_RS09640 (IUJ44_09630) pilT 2051262..2052299 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  IUJ44_RS09645 (IUJ44_09635) - 2052426..2053118 (+) 693 WP_001108520.1 YggS family pyridoxal phosphate-dependent enzyme -
  IUJ44_RS09650 (IUJ44_09640) - 2053319..2056915 (-) 3597 WP_000698801.1 AAA family ATPase -
  IUJ44_RS09655 (IUJ44_09645) - 2056925..2058193 (-) 1269 WP_001984248.1 exonuclease SbcCD subunit D -
  IUJ44_RS09660 (IUJ44_09650) - 2058358..2058831 (+) 474 WP_002134289.1 OsmC family protein -
  IUJ44_RS09665 (IUJ44_09655) - 2058905..2059282 (-) 378 WP_001059437.1 VOC family protein -
  IUJ44_RS09670 (IUJ44_09660) - 2059662..2060114 (+) 453 WP_000164154.1 ABZJ_00895 family protein -
  IUJ44_RS09675 (IUJ44_09665) - 2060203..2061108 (+) 906 WP_001039359.1 TIGR01777 family oxidoreductase -
  IUJ44_RS09680 (IUJ44_09670) - 2061100..2062215 (-) 1116 WP_000546700.1 FAD-dependent oxidoreductase -
  IUJ44_RS09685 (IUJ44_09675) hemB 2062241..2063254 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  IUJ44_RS09690 (IUJ44_09680) - 2063397..2063894 (+) 498 WP_002019577.1 thioesterase family protein -
  IUJ44_RS09695 (IUJ44_09685) - 2064164..2065315 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  IUJ44_RS09700 (IUJ44_09690) - 2065322..2066845 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  IUJ44_RS09705 (IUJ44_09695) ggt 2067149..2069134 (+) 1986 WP_001042928.1 gamma-glutamyltransferase -
  IUJ44_RS09715 (IUJ44_09705) - 2069835..2071064 (+) 1230 WP_002134292.1 tyrosine-type recombinase/integrase -
  IUJ44_RS09720 (IUJ44_09710) - 2071214..2071840 (+) 627 WP_000151565.1 hypothetical protein -
  IUJ44_RS09725 (IUJ44_09715) - 2071968..2072183 (+) 216 WP_000153154.1 hypothetical protein -
  IUJ44_RS09730 (IUJ44_09720) - 2072186..2072395 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  IUJ44_RS09735 (IUJ44_09725) - 2072392..2072946 (+) 555 WP_000991775.1 BRO family protein -
  IUJ44_RS09740 (IUJ44_09730) - 2072939..2073307 (+) 369 WP_001046480.1 hypothetical protein -
  IUJ44_RS09745 (IUJ44_09735) - 2073317..2073628 (+) 312 WP_000787400.1 hypothetical protein -
  IUJ44_RS09750 (IUJ44_09740) - 2073743..2074021 (+) 279 WP_001064706.1 hypothetical protein -
  IUJ44_RS09755 (IUJ44_09745) - 2074033..2076498 (+) 2466 WP_001022433.1 DUF927 domain-containing protein -
  IUJ44_RS09760 (IUJ44_09750) - 2076822..2077025 (+) 204 WP_001984265.1 single-stranded DNA-binding protein -
  IUJ44_RS09765 (IUJ44_09755) - 2077162..2078604 (+) 1443 WP_000003532.1 hypothetical protein -
  IUJ44_RS09770 (IUJ44_09760) - 2078947..2079621 (+) 675 WP_000560650.1 DUF3800 domain-containing protein -
  IUJ44_RS09775 (IUJ44_09765) - 2079621..2079809 (+) 189 WP_000642900.1 hypothetical protein -
  IUJ44_RS09780 (IUJ44_09770) - 2079954..2080529 (+) 576 WP_000067109.1 hypothetical protein -
  IUJ44_RS19085 - 2081001..2081163 (+) 163 Protein_1875 single-stranded DNA-binding protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=501335 IUJ44_RS09640 WP_000355489.1 2051262..2052299(-) (pilT) [Acinetobacter baumannii strain PartI-Abaumannii-RM8376 isolate ATCC isolate]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=501335 IUJ44_RS09640 WP_000355489.1 2051262..2052299(-) (pilT) [Acinetobacter baumannii strain PartI-Abaumannii-RM8376 isolate ATCC isolate]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAGGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTCTTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGTCAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACGGGGCCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACATCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTCCACACCACGTCAGCTGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGTGGCCGTGTCGCAGCGCATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACCACACTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTAATTAGCCCGCAAACGGCTCGTACTGCAGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1
  pilT Acinetobacter baumannii D1279779

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388