Detailed information NTG0501003

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: JKN64_RS01350
• Genome accession: NZ_CP076245
• Coordinates: 279648..280631 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain Balcarce_24.2
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 274648..285631

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JKN64_RS01335 (JKN64_01325)	dppA	275948..277555 (+)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-
JKN64_RS01340 (JKN64_01330)	dppB	277706..278725 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
JKN64_RS01345 (JKN64_01335)	dppC	278735..279637 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
JKN64_RS01350 (JKN64_01340)	amiE	279648..280631 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
JKN64_RS01355 (JKN64_01345)	dppF	280628..281632 (+)	1005	WP_046425670.1	dipeptide ABC transporter ATP-binding subunit DppF	-
JKN64_RS01360 (JKN64_01350)	yhjV	281662..282933 (-)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
JKN64_RS01365 (JKN64_01355)	ldrD	283409..283516 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
JKN64_RS01370 (JKN64_01360)	bcsG	283603..285282 (-)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
JKN64_RS01375 (JKN64_01365)	bcsF	285279..285470 (-)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=501003 JKN64_RS01350 WP_001196486.1 279648..280631(+) (amiE) [Escherichia coli O157:H7 strain Balcarce_24.2]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=501003 JKN64_RS01350 WP_001196486.1 279648..280631(+) (amiE) [Escherichia coli O157:H7 strain Balcarce_24.2]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404