Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IS481_RS17205 Genome accession   NZ_CP064338
Coordinates   3637316..3638359 (-) Length   347 a.a.
NCBI ID   WP_104356388.1    Uniprot ID   A0A2S5T8C7
Organism   Caldimonas thermodepolymerans strain DSM 15344     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3632316..3643359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS481_RS17175 (IS481_17175) - 3632633..3633001 (+) 369 WP_104356382.1 YraN family protein -
  IS481_RS17180 (IS481_17180) - 3633129..3633725 (+) 597 WP_104356383.1 phosphoheptose isomerase -
  IS481_RS17185 (IS481_17185) - 3633722..3634405 (+) 684 WP_232529351.1 BON domain-containing protein -
  IS481_RS17190 (IS481_17190) - 3634473..3635366 (-) 894 WP_104356385.1 NAD(P)-dependent oxidoreductase -
  IS481_RS17195 (IS481_17195) pilU 3635434..3636570 (-) 1137 WP_104356386.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IS481_RS17200 (IS481_17200) - 3636623..3637276 (-) 654 WP_104356387.1 cyclic nucleotide-binding domain-containing protein -
  IS481_RS17205 (IS481_17205) pilT 3637316..3638359 (-) 1044 WP_104356388.1 type IV pilus twitching motility protein PilT Machinery gene
  IS481_RS17210 (IS481_17210) - 3638434..3639120 (+) 687 WP_104356389.1 YggS family pyridoxal phosphate-dependent enzyme -
  IS481_RS17215 (IS481_17215) - 3639136..3639618 (-) 483 WP_104356390.1 Lrp/AsnC ligand binding domain-containing protein -
  IS481_RS17220 (IS481_17220) - 3639716..3642724 (+) 3009 WP_232529353.1 L-glutamate gamma-semialdehyde dehydrogenase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38161.96 Da        Isoelectric Point: 7.2737

>NTDB_id=500860 IS481_RS17205 WP_104356388.1 3637316..3638359(-) (pilT) [Caldimonas thermodepolymerans strain DSM 15344]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKQVHDMVYDIMNDAQRKAYEETLECDFSFEIQGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNAPKVFAELALKPRGLVLVTGPTGSGKSTTLAAMINHLNENQYGHVLTVED
PIEFVHESKKCLVNQREVGPHTLSFANALRSALREDPDAILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAAEKEMVRAMLSESLVAVISQTLCKLKDGSGRIAAHEIMLGTAAIRNLIRENKIAQMYSAIQTGNSMGMQTLDQC
LSDLVRRNLISPAEARAKAKFPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=500860 IS481_RS17205 WP_104356388.1 3637316..3638359(-) (pilT) [Caldimonas thermodepolymerans strain DSM 15344]
ATGGACATCACCCAGCTGCTGGCCTTCTCGGTCAAGAACAAGGCATCCGACCTGCACCTGTCTGCCGGCCTGCCGCCGAT
GATTCGCGTGCATGGCGACGTGCGTCGCATCAATGTCGACCCGCTGGACCACAAGCAGGTCCACGACATGGTGTACGACA
TCATGAACGACGCCCAGCGCAAGGCCTACGAGGAGACGCTGGAGTGCGACTTCTCGTTCGAGATCCAGGGGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGGGGCGCGGGCGCCGTGTTCCGGACCATCCCGTCCAAGATCCTCACGCTCGA
GCAGCTCAACGCGCCCAAGGTGTTCGCCGAGCTCGCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CCGGCAAGTCCACCACCCTGGCGGCGATGATCAACCACCTCAACGAGAACCAGTACGGCCACGTGCTGACGGTGGAGGAC
CCGATCGAGTTCGTGCACGAATCCAAGAAGTGCCTGGTCAACCAGCGCGAGGTGGGCCCGCACACGCTGAGCTTCGCCAA
CGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACGCGATCCTGGTGGGCGAGATGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACTTCGAGCGCGGCCAAGACCATCGACCGCATC
GTCGACGTGTTCCCCGCGGCCGAGAAGGAGATGGTGCGCGCGATGCTGTCCGAGTCGCTGGTCGCGGTGATCTCGCAGAC
GCTGTGCAAGCTCAAGGACGGCTCGGGCCGCATCGCGGCGCACGAGATCATGCTGGGCACGGCGGCCATCCGCAACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCCGCGATCCAGACCGGCAACAGCATGGGCATGCAGACGCTGGACCAGTGC
CTGTCCGACCTGGTGCGCCGCAACCTGATTTCGCCGGCCGAAGCGCGCGCCAAGGCCAAGTTCCCGGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S5T8C7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.681

99.424

0.772
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

73.623

99.424

0.732
  pilT Legionella pneumophila strain Lp02

72.271

97.695

0.706
  pilT Legionella pneumophila strain ERS1305867

72.271

97.695

0.706
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.696

99.424

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.272

97.406

0.646
  pilT Vibrio cholerae strain A1552

66.272

97.406

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

42.687

96.542

0.412
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406