Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IS507_RS04510 Genome accession   NZ_CP064194
Coordinates   929689..930726 (-) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain X4-65     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 887253..954168 929689..930726 within 0


Gene organization within MGE regions


Location: 887253..954168
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IS507_RS04300 - 887888..889513 (-) 1626 WP_000834616.1 NAD+ synthase -
  IS507_RS04305 - 889898..890458 (-) 561 WP_001048150.1 hypothetical protein -
  IS507_RS04310 - 890549..891409 (-) 861 WP_000838098.1 pirin family protein -
  IS507_RS04315 hemJ 891580..892032 (-) 453 WP_000339889.1 protoporphyrinogen oxidase HemJ -
  IS507_RS04320 - 892051..893280 (-) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  IS507_RS04325 - 893577..894902 (+) 1326 WP_000160011.1 EcsC family protein -
  IS507_RS04330 rpsL 895103..895477 (+) 375 WP_000246374.1 30S ribosomal protein S12 -
  IS507_RS04335 rpsG 895637..896107 (+) 471 WP_001138055.1 30S ribosomal protein S7 -
  IS507_RS04340 fusA 896286..898424 (+) 2139 WP_000113824.1 elongation factor G -
  IS507_RS04345 tuf 898519..899709 (+) 1191 WP_001029610.1 elongation factor Tu -
  IS507_RS04350 - 899913..900794 (+) 882 WP_000992305.1 metal-dependent hydrolase -
  IS507_RS04355 - 901031..901906 (+) 876 WP_000992291.1 metal-dependent hydrolase -
  IS507_RS04360 rimI 902015..902470 (+) 456 WP_000621543.1 ribosomal protein S18-alanine N-acetyltransferase -
  IS507_RS04365 - 902515..903330 (-) 816 WP_000844343.1 arginyltransferase -
  IS507_RS04370 aat 903349..904080 (-) 732 WP_000854785.1 leucyl/phenylalanyl-tRNA--protein transferase -
  IS507_RS04375 trxB 904200..905147 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  IS507_RS04380 - 905417..908449 (+) 3033 WP_000127823.1 DNA translocase FtsK -
  IS507_RS04385 rhtC 908599..909219 (+) 621 WP_000959259.1 threonine export protein RhtC -
  IS507_RS04390 - 909275..909697 (-) 423 Protein_829 hypothetical protein -
  IS507_RS04395 - 909731..911377 (-) 1647 WP_001094416.1 IS66-like element ISAba17 family transposase -
  IS507_RS04400 tnpB 911452..911787 (-) 336 WP_000618090.1 IS66 family insertion sequence element accessory protein TnpB -
  IS507_RS04405 - 911784..912212 (-) 429 WP_000364662.1 transposase -
  IS507_RS04410 - 912294..912422 (-) 129 Protein_833 hypothetical protein -
  IS507_RS04415 - 912849..913136 (-) 288 WP_001218560.1 PA4642 family protein -
  IS507_RS04420 minE 913220..913492 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  IS507_RS04425 minD 913495..914307 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  IS507_RS04430 minC 914378..915100 (-) 723 WP_000763677.1 septum site-determining protein MinC -
  IS507_RS04435 - 915222..916268 (-) 1047 WP_001181639.1 hypothetical protein -
  IS507_RS04440 - 916285..917211 (-) 927 WP_000100965.1 acyltransferase -
  IS507_RS04445 - 917456..918109 (+) 654 WP_001202415.1 OmpA family protein -
  IS507_RS04450 rep 918303..920342 (+) 2040 WP_000093035.1 DNA helicase Rep -
  IS507_RS04455 dut 920367..920819 (+) 453 WP_000868152.1 dUTP diphosphatase -
  IS507_RS04460 - 921019..922437 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  IS507_RS04465 argB 922452..923360 (+) 909 WP_001135419.1 acetylglutamate kinase -
  IS507_RS04470 - 923481..924263 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  IS507_RS04475 - 924372..925220 (+) 849 WP_000336548.1 class II glutamine amidotransferase -
  IS507_RS04480 - 925473..925928 (+) 456 WP_000782976.1 bacteriohemerythrin -
  IS507_RS04485 - 926081..927148 (+) 1068 WP_002019572.1 hypothetical protein -
  IS507_RS04490 - 927148..927471 (+) 324 WP_000442587.1 RnfH family protein -
  IS507_RS04495 bamE 927526..927924 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  IS507_RS04500 fur 928036..928473 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  IS507_RS04505 pilU 928543..929661 (-) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IS507_RS04510 pilT 929689..930726 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  IS507_RS04515 - 930853..931545 (+) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  IS507_RS04520 - 931747..935343 (-) 3597 WP_000698785.1 SbcC/MukB-like Walker B domain-containing protein -
  IS507_RS04525 - 935353..936621 (-) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  IS507_RS04530 - 936785..937258 (+) 474 WP_000974358.1 OsmC family protein -
  IS507_RS04535 - 937326..937703 (-) 378 WP_001059438.1 VOC family protein -
  IS507_RS04540 - 938083..938535 (+) 453 WP_000164153.1 ABZJ_00895 family protein -
  IS507_RS04545 - 938625..939530 (+) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  IS507_RS04550 - 939522..940637 (-) 1116 WP_000546701.1 FAD-dependent oxidoreductase -
  IS507_RS04555 hemB 940663..941676 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  IS507_RS04560 - 941819..942316 (+) 498 WP_002019577.1 thioesterase family protein -
  IS507_RS04565 - 942586..943737 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  IS507_RS04570 - 943744..945267 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  IS507_RS04575 ggt 945570..947555 (+) 1986 WP_001042923.1 gamma-glutamyltransferase -
  IS507_RS04585 - 948257..949486 (+) 1230 WP_001991065.1 tyrosine-type recombinase/integrase -
  IS507_RS04590 - 949634..950401 (+) 768 WP_000578618.1 hypothetical protein -
  IS507_RS04595 - 950611..950826 (+) 216 WP_000153154.1 hypothetical protein -
  IS507_RS04600 - 950829..951038 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  IS507_RS04605 - 951035..951589 (+) 555 WP_001988245.1 BRO family protein -
  IS507_RS04610 - 951582..951950 (+) 369 WP_001046481.1 hypothetical protein -
  IS507_RS04615 - 951960..952262 (+) 303 Protein_873 hypothetical protein -
  IS507_RS04620 - 952390..952668 (+) 279 WP_001064707.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=500046 IS507_RS04510 WP_000355489.1 929689..930726(-) (pilT) [Acinetobacter baumannii strain X4-65]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=500046 IS507_RS04510 WP_000355489.1 929689..930726(-) (pilT) [Acinetobacter baumannii strain X4-65]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCCGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CAGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTACACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTACTCAGCCATTCAAACTGGTGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGCCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1
  pilT Acinetobacter baumannii D1279779

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388