Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ISX58_RS03750 Genome accession   NZ_CP064180
Coordinates   764978..765958 (+) Length   326 a.a.
NCBI ID   WP_044255660.1    Uniprot ID   -
Organism   Citrobacter amalonaticus strain CA71     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 759978..770958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ISX58_RS03720 (ISX58_03720) - 760615..761622 (+) 1008 WP_194694192.1 DUF1202 family protein -
  ISX58_RS03725 (ISX58_03725) hemW 761654..762790 (-) 1137 WP_042998064.1 radical SAM family heme chaperone HemW -
  ISX58_RS03730 (ISX58_03730) - 762783..763376 (-) 594 WP_042998063.1 XTP/dITP diphosphatase -
  ISX58_RS03735 (ISX58_03735) yggU 763384..763674 (-) 291 WP_044255664.1 DUF167 family protein YggU -
  ISX58_RS03740 (ISX58_03740) - 763671..764237 (-) 567 WP_042998061.1 YggT family protein -
  ISX58_RS03745 (ISX58_03745) - 764256..764960 (-) 705 WP_044255662.1 YggS family pyridoxal phosphate-dependent enzyme -
  ISX58_RS03750 (ISX58_03750) pilT 764978..765958 (+) 981 WP_044255660.1 type IV pilus twitching motility protein PilT Machinery gene
  ISX58_RS03755 (ISX58_03755) ruvX 765972..766388 (-) 417 WP_042998058.1 Holliday junction resolvase RuvX -
  ISX58_RS03760 (ISX58_03760) - 766388..766951 (-) 564 WP_042998057.1 YqgE/AlgH family protein -
  ISX58_RS03765 (ISX58_03765) gshB 767126..768073 (-) 948 WP_044255655.1 glutathione synthase -
  ISX58_RS03770 (ISX58_03770) rsmE 768086..768817 (-) 732 WP_042998055.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ISX58_RS03775 (ISX58_03775) endA 768897..769604 (-) 708 WP_042998054.1 deoxyribonuclease I -
  ISX58_RS03780 (ISX58_03780) - 769696..770196 (-) 501 WP_044255652.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35848.87 Da        Isoelectric Point: 7.0211

>NTDB_id=499993 ISX58_RS03750 WP_044255660.1 764978..765958(+) (pilT) [Citrobacter amalonaticus strain CA71]
MNMEEIVALSVKHNVSDLHLSNAWPARWRKRGRMERAPFTATDITGQLADWLDEGQQAALRQNGQLDFAVSLADNQRLRA
SAFHQRQGTSLALRLLPTQCPTLETLGTPSVLPELLRSENGLILVTGATGSGKSTTLAAMVTWLNQHIDGHILTLEDPIE
YIYTSQRCLIQQREAGLHCASFATGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLIDS
FPAQEKDPVRSQLAGSLRAVLSQKLEPDKQEGRVALYELLINTPAAGNLIREGKSHQLPHVIQTGQQMGMMTFAQSLQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=499993 ISX58_RS03750 WP_044255660.1 764978..765958(+) (pilT) [Citrobacter amalonaticus strain CA71]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTAAGCAATGCGTGGCCTGCACG
CTGGCGAAAACGGGGACGCATGGAGCGTGCACCCTTTACCGCAACCGACATTACCGGACAGCTGGCGGACTGGCTGGATG
AGGGGCAGCAGGCGGCGTTACGACAAAATGGGCAACTGGATTTCGCGGTTTCGCTCGCGGATAACCAGCGCCTGCGGGCC
AGCGCCTTTCACCAGCGGCAGGGGACGTCGTTGGCGCTACGGCTGCTGCCGACGCAGTGCCCGACGCTGGAAACGCTGGG
GACGCCTTCTGTGCTGCCCGAACTGCTCCGCAGCGAGAATGGTTTGATTCTGGTGACTGGGGCGACAGGCAGCGGAAAAT
CAACTACCCTAGCGGCGATGGTCACGTGGCTGAATCAGCATATCGATGGACATATTCTGACGCTGGAAGATCCCATCGAG
TATATCTACACCAGCCAGCGCTGCCTGATTCAGCAGCGTGAAGCAGGACTGCACTGCGCCTCATTTGCCACCGGATTACG
CGCGGCGCTGCGTGAAGATCCGGATGTGATTCTGCTGGGAGAGCTACGCGACAGCGAGACCATTCGTCTGGCGCTAACGG
CGGCGGAAACGGGGCATCTGGTGCTGGCGACGCTGCATACGCGCGGTGCCGCGCAGGCGATCGAGCGACTGATTGATTCT
TTTCCGGCGCAGGAAAAAGACCCGGTGCGCAGCCAACTGGCAGGCAGTCTGCGCGCCGTACTGTCGCAGAAACTGGAACC
CGACAAGCAGGAGGGGCGCGTTGCGCTGTATGAACTGCTGATCAACACGCCGGCAGCGGGGAATTTGATTCGGGAAGGGA
AGTCCCATCAGTTACCCCATGTCATTCAGACCGGGCAGCAGATGGGGATGATGACCTTTGCCCAGAGCCTGCAACAGCGA
CAGGCACAAGGACGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Legionella pneumophila strain Lp02

45.963

98.773

0.454
  pilT Legionella pneumophila strain ERS1305867

45.963

98.773

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.587

97.239

0.414
  pilU Pseudomonas stutzeri DSM 10701

39.697

100

0.402
  pilU Vibrio cholerae strain A1552

42.258

95.092

0.402
  pilU Acinetobacter baylyi ADP1

39.385

99.693

0.393