NTDB

Overview

Name	pilA/pilAI	Type	Machinery gene
Locus tag	ISN31_RS00470	Genome accession	NZ_CP064004
Coordinates	98058..98489 (+)	Length	143 a.a.
NCBI ID	WP_060744724.1	Uniprot ID	A0A120EV69
Organism	Xanthomonas translucens pv. translucens strain XtKm8
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 93058..103489

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ISN31_RS00450 (ISN31_00450)	-	93522..94889 (+)	1368	WP_206711727.1	IS5 family transposase	-
ISN31_RS00455 (ISN31_00455)	coaE	94914..95534 (-)	621	WP_058360087.1	dephospho-CoA kinase	-
ISN31_RS00460 (ISN31_00460)	-	95548..96411 (-)	864	WP_060748002.1	A24 family peptidase	-
ISN31_RS00465 (ISN31_00465)	pilC	96418..97674 (-)	1257	WP_060748003.1	type II secretion system F family protein	Machinery gene
ISN31_RS00470 (ISN31_00470)	pilA/pilAI	98058..98489 (+)	432	WP_060744724.1	pilin	Machinery gene
ISN31_RS00475 (ISN31_00475)	-	98574..98972 (-)	399	WP_196484464.1	hypothetical protein	-
ISN31_RS00480 (ISN31_00480)	pilB	99235..100971 (+)	1737	WP_058363696.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ISN31_RS00485 (ISN31_00485)	-	100999..101298 (-)	300	WP_237653908.1	addiction module antidote protein	-
ISN31_RS00490 (ISN31_00490)	-	101295..101590 (-)	296	Protein_97	type II toxin-antitoxin system RelE/ParE family toxin	-
ISN31_RS00495 (ISN31_00495)	pilR	101755..103143 (-)	1389	WP_060748005.1	sigma-54 dependent transcriptional regulator	Regulator

Sequence

Protein

Download Length: 143 a.a. Molecular weight: 14990.20 Da Isoelectric Point: 8.4700

                                >NTDB_id=498486 ISN31_RS00470 WP_060744724.1 98058..98489(+) (pilA/pilAI) [Xanthomonas translucens pv. translucens strain XtKm8]

MKKQQGFTLIELMIVVAIIAILAAIAIPMYQDYVVKSQATSALAEITPGKVGFETAINQGKTPSTTATDDGYIGIPTTTT

YCNVGLGTTATGNITCTAKGGNASKFNTKKIIWTRDDNGAWTCTSDLDAKYRPGSCGVVAATK

Nucleotide

Download Length: 432 bp

                                >NTDB_id=498486 ISN31_RS00470 WP_060744724.1 98058..98489(+) (pilA/pilAI) [Xanthomonas translucens pv. translucens strain XtKm8]

ATGAAGAAGCAACAGGGCTTTACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGCGATCTTGGCCGCCATCGCAAT

CCCGATGTACCAGGACTATGTTGTCAAGTCGCAAGCTACCTCGGCGCTGGCCGAAATCACCCCGGGCAAGGTTGGGTTCG

AAACCGCGATCAATCAAGGCAAGACGCCCAGCACCACTGCTACGGATGACGGCTATATTGGTATCCCAACCACGACCACC

TATTGCAACGTTGGCCTGGGTACCACTGCCACTGGCAATATCACCTGTACCGCAAAGGGTGGTAATGCCAGTAAGTTCAA

TACCAAGAAGATCATTTGGACGCGTGACGACAATGGCGCATGGACTTGCACCAGTGATCTGGATGCCAAGTATCGTCCCG

GTAGCTGTGGTGTCGTTGCTGCTACGAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-136)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A120EV69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	68.421	93.007	0.636
pilA/pilAII	Pseudomonas stutzeri DSM 10701	59.091	92.308	0.545
pilA	Pseudomonas aeruginosa PAK	46.622	100	0.483
pilA	Acinetobacter baumannii strain A118	47.518	98.601	0.469
pilA2	Legionella pneumophila strain ERS1305867	44.928	96.503	0.434
pilA2	Legionella pneumophila str. Paris	44.928	96.503	0.434
pilA	Ralstonia pseudosolanacearum GMI1000	37.126	100	0.434
pilA	Vibrio cholerae C6706	42.857	97.902	0.42
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.857	97.902	0.42
pilA	Vibrio cholerae strain A1552	42.857	97.902	0.42
comP	Acinetobacter baylyi ADP1	38.816	100	0.413
pilE	Neisseria gonorrhoeae MS11	35.256	100	0.385
pilA	Haemophilus influenzae Rd KW20	38.028	99.301	0.378
pilE	Neisseria gonorrhoeae strain FA1090	34.177	100	0.378

Detailed information NTG0498486