NTDB

Overview

Name	pilA/pilAI	Type	Machinery gene
Locus tag	ISN34_RS17315	Genome accession	NZ_CP064001
Coordinates	4009633..4010064 (-)	Length	143 a.a.
NCBI ID	WP_060744724.1	Uniprot ID	A0A120EV69
Organism	Xanthomonas translucens pv. translucens strain XtKm34
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4004633..4015064

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ISN34_RS17290 (ISN34_17270)	pilR	4004979..4006367 (+)	1389	WP_206710787.1	sigma-54 dependent transcriptional regulator	Regulator
ISN34_RS17295 (ISN34_17275)	-	4006532..4006827 (+)	296	Protein_3429	type II toxin-antitoxin system RelE/ParE family toxin	-
ISN34_RS17300 (ISN34_17280)	-	4006824..4007123 (+)	300	WP_237653908.1	addiction module antidote protein	-
ISN34_RS17305 (ISN34_17285)	pilB	4007151..4008887 (-)	1737	WP_058363696.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ISN34_RS17310 (ISN34_17290)	-	4009150..4009548 (+)	399	WP_196484464.1	hypothetical protein	-
ISN34_RS17315 (ISN34_17295)	pilA/pilAI	4009633..4010064 (-)	432	WP_060744724.1	pilin	Machinery gene
ISN34_RS17320 (ISN34_17300)	pilC	4010448..4011704 (+)	1257	WP_060748003.1	type II secretion system F family protein	Machinery gene
ISN34_RS17325 (ISN34_17305)	-	4011711..4012574 (+)	864	WP_060748002.1	A24 family peptidase	-
ISN34_RS17330 (ISN34_17310)	coaE	4012588..4013208 (+)	621	WP_058360087.1	dephospho-CoA kinase	-
ISN34_RS17335 (ISN34_17315)	-	4013233..4014602 (-)	1370	Protein_3437	IS5 family transposase	-

Sequence

Protein

Download Length: 143 a.a. Molecular weight: 14990.20 Da Isoelectric Point: 8.4700

                                >NTDB_id=498450 ISN34_RS17315 WP_060744724.1 4009633..4010064(-) (pilA/pilAI) [Xanthomonas translucens pv. translucens strain XtKm34]

MKKQQGFTLIELMIVVAIIAILAAIAIPMYQDYVVKSQATSALAEITPGKVGFETAINQGKTPSTTATDDGYIGIPTTTT

YCNVGLGTTATGNITCTAKGGNASKFNTKKIIWTRDDNGAWTCTSDLDAKYRPGSCGVVAATK

Nucleotide

Download Length: 432 bp

                                >NTDB_id=498450 ISN34_RS17315 WP_060744724.1 4009633..4010064(-) (pilA/pilAI) [Xanthomonas translucens pv. translucens strain XtKm34]

ATGAAGAAGCAACAGGGCTTTACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGCGATCTTGGCCGCCATCGCAAT

CCCGATGTACCAGGACTATGTTGTCAAGTCGCAAGCTACCTCGGCGCTGGCCGAAATCACCCCGGGCAAGGTTGGGTTCG

AAACCGCGATCAATCAAGGCAAGACGCCCAGCACCACTGCTACGGATGACGGCTATATTGGTATCCCAACCACGACCACC

TATTGCAACGTTGGCCTGGGTACCACTGCCACTGGCAATATCACCTGTACCGCAAAGGGTGGTAATGCCAGTAAGTTCAA

TACCAAGAAGATCATTTGGACGCGTGACGACAATGGCGCATGGACTTGCACCAGTGATCTGGATGCCAAGTATCGTCCCG

GTAGCTGTGGTGTCGTTGCTGCTACGAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-136)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A120EV69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA/pilAI	Pseudomonas stutzeri DSM 10701	68.421	93.007	0.636
pilA/pilAII	Pseudomonas stutzeri DSM 10701	59.091	92.308	0.545
pilA	Pseudomonas aeruginosa PAK	46.622	100	0.483
pilA	Acinetobacter baumannii strain A118	47.518	98.601	0.469
pilA2	Legionella pneumophila strain ERS1305867	44.928	96.503	0.434
pilA2	Legionella pneumophila str. Paris	44.928	96.503	0.434
pilA	Ralstonia pseudosolanacearum GMI1000	37.126	100	0.434
pilA	Vibrio cholerae C6706	42.857	97.902	0.42
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.857	97.902	0.42
pilA	Vibrio cholerae strain A1552	42.857	97.902	0.42
comP	Acinetobacter baylyi ADP1	38.816	100	0.413
pilE	Neisseria gonorrhoeae MS11	35.256	100	0.385
pilA	Haemophilus influenzae Rd KW20	38.028	99.301	0.378
pilE	Neisseria gonorrhoeae strain FA1090	34.177	100	0.378

Detailed information NTG0498450