Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IG390_RS05655 Genome accession   NZ_CP063965
Coordinates   1179361..1180416 (+) Length   351 a.a.
NCBI ID   WP_039257532.1    Uniprot ID   A0A0A0IM70
Organism   Clostridium botulinum strain BKT2873     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1174361..1185416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG390_RS05635 (IG390_05625) - 1174880..1176106 (+) 1227 WP_039257528.1 U32 family peptidase -
  IG390_RS05640 (IG390_05630) udk 1176110..1176736 (+) 627 WP_039257529.1 uridine kinase -
  IG390_RS05645 (IG390_05635) - 1176801..1178486 (+) 1686 WP_039259015.1 penicillin-binding transpeptidase domain-containing protein -
  IG390_RS05650 (IG390_05640) sigK 1178553..1179260 (+) 708 WP_039257531.1 RNA polymerase sporulation sigma factor SigK -
  IG390_RS05655 (IG390_05645) pilT 1179361..1180416 (+) 1056 WP_039257532.1 type IV pilus twitching motility protein PilT Machinery gene
  IG390_RS05660 (IG390_05650) ftsA 1180668..1181924 (+) 1257 WP_039257533.1 cell division protein FtsA -
  IG390_RS05665 (IG390_05655) ftsZ 1181940..1183106 (+) 1167 WP_039257534.1 cell division protein FtsZ -
  IG390_RS05670 (IG390_05660) spoIIGA 1183286..1184077 (+) 792 WP_039257535.1 sigma-E processing peptidase SpoIIGA -
  IG390_RS05675 (IG390_05665) sigE 1184091..1184798 (+) 708 WP_029169687.1 RNA polymerase sporulation sigma factor SigE -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39029.19 Da        Isoelectric Point: 8.9717

>NTDB_id=498186 IG390_RS05655 WP_039257532.1 1179361..1180416(+) (pilT) [Clostridium botulinum strain BKT2873]
MIPLNELLKKTIDEKASDLHLTVGISPVIRINGELVRVGEQKLTANDTRKYVMEILGDSWEKYDNVGEIDTSISIPSVGR
FRVNIYKQRGSYALAIRAVALKIPSISQLKLPSIIKEISDKHRGLILVTGPTGSGKSTTLASIINEINNTRSAHIITLED
PIEFLHKHNKSIINQREIGNDTSSYTDALRAVLREDPDVILVGEMRDLETMATAITAAETGHLVLSTLHTIGAAKTIDRI
IDVFPPHQQQQIKIQLSTVLQGVISQQLIPKYDGKGQIASFEIMVLNSAIQNLIREGKTHQIQSLIQTGSKYGMKTMDMS
IVELYKSKLISYENALTYSIDKSMVKRMIDM

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=498186 IG390_RS05655 WP_039257532.1 1179361..1180416(+) (pilT) [Clostridium botulinum strain BKT2873]
GTGATACCATTAAATGAATTATTAAAAAAGACTATAGATGAAAAAGCATCAGATCTCCATTTAACTGTTGGAATTTCTCC
GGTAATTAGAATAAATGGTGAACTAGTAAGGGTTGGTGAGCAAAAGCTTACAGCAAATGATACAAGAAAATATGTTATGG
AAATATTGGGGGACTCATGGGAAAAATATGATAATGTTGGTGAAATAGATACATCTATATCTATTCCTAGTGTTGGAAGA
TTTAGAGTTAATATATATAAACAGAGGGGGAGTTATGCACTTGCTATAAGAGCAGTTGCATTAAAAATACCTTCTATTAG
TCAATTAAAGTTACCCTCTATAATAAAAGAGATTAGTGATAAACATAGAGGACTTATTCTTGTAACAGGACCAACTGGAA
GTGGTAAGAGTACAACTTTAGCATCTATAATAAATGAGATAAATAATACGAGATCAGCTCATATTATTACATTAGAAGAT
CCTATAGAATTTCTTCATAAGCATAATAAGTCAATAATTAATCAAAGGGAAATAGGAAACGATACATCATCTTATACTGA
TGCCCTAAGAGCAGTACTTAGAGAGGATCCAGATGTAATTCTTGTTGGGGAGATGAGAGATCTTGAAACTATGGCAACGG
CAATTACAGCAGCAGAAACAGGACATTTGGTATTATCTACTCTACATACTATAGGAGCAGCTAAAACTATAGATAGAATA
ATTGATGTTTTTCCACCACATCAACAACAACAAATAAAAATACAACTTTCAACTGTACTTCAAGGTGTTATATCTCAACA
ATTAATACCAAAATATGACGGAAAAGGACAAATAGCTTCATTTGAAATTATGGTATTAAACTCTGCTATACAAAATCTTA
TAAGAGAAGGGAAAACTCACCAAATTCAATCATTAATTCAAACAGGAAGTAAGTATGGTATGAAAACTATGGATATGAGC
ATAGTTGAGCTATATAAAAGTAAATTAATATCCTATGAGAATGCTTTAACGTATTCTATTGACAAAAGTATGGTAAAAAG
AATGATAGATATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(93-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A0IM70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.751

94.872

0.481
  pilT Neisseria gonorrhoeae MS11

50.45

94.872

0.479
  pilT Acinetobacter nosocomialis M2

49.254

95.442

0.47
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.143

99.715

0.47
  pilT Acinetobacter baumannii strain A118

48.961

96.011

0.47
  pilT Acinetobacter baumannii D1279779

48.961

96.011

0.47
  pilT Legionella pneumophila strain Lp02

48.094

97.151

0.467
  pilT Legionella pneumophila strain ERS1305867

48.094

97.151

0.467
  pilT Pseudomonas aeruginosa PAK

48.649

94.872

0.462
  pilT Acinetobacter baylyi ADP1

48.649

94.872

0.462
  pilT Vibrio cholerae strain A1552

49.688

91.168

0.453
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.688

91.168

0.453
  pilT Pseudomonas stutzeri DSM 10701

46.847

94.872

0.444
  pilU Acinetobacter baylyi ADP1

41.42

96.296

0.399
  pilU Vibrio cholerae strain A1552

39.359

97.721

0.385
  pilU Pseudomonas stutzeri DSM 10701

38.682

99.43

0.385