Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IGI31_RS03915 Genome accession   NZ_CP075465
Coordinates   800204..801184 (+) Length   326 a.a.
NCBI ID   WP_071999886.1    Uniprot ID   -
Organism   Escherichia coli strain NT1N34     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795204..806184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IGI31_RS03885 yggM 795719..796726 (+) 1008 WP_032237482.1 DUF1202 family protein -
  IGI31_RS03890 hemW 796881..798017 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  IGI31_RS03895 rdgB 798010..798603 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  IGI31_RS03900 yggU 798611..798901 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  IGI31_RS03905 yggT 798898..799464 (-) 567 WP_032237483.1 osmotic shock tolerance protein YggT -
  IGI31_RS03910 yggS 799482..800186 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  IGI31_RS03915 pilT 800204..801184 (+) 981 WP_071999886.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IGI31_RS03920 ruvX 801359..801775 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  IGI31_RS03925 yqgE 801775..802338 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  IGI31_RS03930 gshB 802447..803397 (-) 951 WP_000593273.1 glutathione synthase -
  IGI31_RS03935 rsmE 803410..804141 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IGI31_RS03940 endA 804221..804928 (-) 708 WP_000286493.1 deoxyribonuclease I -
  IGI31_RS03945 yggI 805023..805520 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35976.14 Da        Isoelectric Point: 5.7980

>NTDB_id=498042 IGI31_RS03915 WP_071999886.1 800204..801184(+) (pilT) [Escherichia coli strain NT1N34]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=498042 IGI31_RS03915 WP_071999886.1 800204..801184(+) (pilT) [Escherichia coli strain NT1N34]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362