Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HGR01_RS21655 Genome accession   NZ_CP063785
Coordinates   5267233..5268357 (-) Length   374 a.a.
NCBI ID   WP_045874068.1    Uniprot ID   A0A0D6KCX9
Organism   Tolypothrix sp. PCC 7712     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5262233..5273357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGR01_RS21630 (HGR01_21005) - 5263238..5263408 (+) 171 WP_309227650.1 DUF305 domain-containing protein -
  HGR01_RS21635 (HGR01_21010) - 5263437..5263631 (+) 195 WP_045874072.1 heavy-metal-associated domain-containing protein -
  HGR01_RS21640 (HGR01_21015) bioU 5263884..5264906 (+) 1023 WP_045874071.1 (S)-8-amino-7-oxononanoate synthase BioU -
  HGR01_RS21645 (HGR01_21020) - 5265125..5265682 (-) 558 WP_045874070.1 hypothetical protein -
  HGR01_RS21650 (HGR01_21025) - 5265786..5267006 (-) 1221 WP_045874069.1 type II secretion system F family protein -
  HGR01_RS21655 (HGR01_21030) pilT 5267233..5268357 (-) 1125 WP_045874068.1 type IV pilus twitching motility protein PilT Machinery gene
  HGR01_RS21660 (HGR01_21035) pilB/pilB1 5268403..5270418 (-) 2016 WP_045874067.1 GspE/PulE family protein Machinery gene
  HGR01_RS21665 (HGR01_21040) grpE 5270838..5271584 (+) 747 WP_045874066.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40967.35 Da        Isoelectric Point: 7.4766

>NTDB_id=496801 HGR01_RS21655 WP_045874068.1 5267233..5268357(-) (pilT) [Tolypothrix sp. PCC 7712]
MEMMIEDLMEQMIEMGGSDLHLSAGLPPYFRISGKLTPIGDHVLTVDQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKDRGSYAACLRALSSKIPNFEKLGLPDVVREMTEKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMIITPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKAGTISFEAAMSKTSKPDEIQRLIGGAPQAAGAKPAAGVAKAH

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=496801 HGR01_RS21655 WP_045874068.1 5267233..5268357(-) (pilT) [Tolypothrix sp. PCC 7712]
ATGGAGATGATGATTGAAGATTTGATGGAGCAGATGATTGAAATGGGGGGTTCAGATCTGCATTTATCCGCCGGTTTACC
ACCCTATTTCCGCATTAGTGGCAAACTCACCCCCATCGGCGACCATGTATTGACTGTGGATCAGTGTCAAAGGCTGATCT
TTAGTATGCTCAACAATACTCAGCGTAAAACTTTAGAGCAGACCTGGGAACTCGATTGTTCTTATGGTGTAAAAGGCTTG
GCGCGGTTCCGCGTCAATGTTTATAAGGATCGTGGTTCCTACGCTGCTTGTTTACGCGCCCTCAGCTCCAAAATTCCCAA
CTTTGAAAAATTAGGGTTGCCAGATGTAGTCCGGGAAATGACAGAAAAGCCCAGAGGACTGATTTTGGTGACGGGGCCCA
CTGGTTCTGGTAAGACCACTACCTTGGCAGCAATGATTGACTTGATTAACCGCACCAAGGCAGAGCATATTTTGACAGTA
GAAGATCCAATTGAATTTGTGTATGAACCAATTAAAAGCTTGGTTCACCAACGACAACTTGGTGAAGATACTAAGAGTTT
CGCTAATGCTTTGAAGGCAGCTTTGCGGGAAGATCCAGATATTATTCTGGTGGGGGAAATGCGGGATTTGGAAACGATTT
CCTTGGCGATTTCTGCGGCGGAAACAGGTCACTTAGTATTTGGAACTTTGCACACCAGTTCAGCGGCACAGACGGTTGAC
CGGATTATTGACGTTTTTCCCCATGAAAGACAAACCCAAGTAAGGGTGCAGTTATCTAACTCCTTGGTAGCTGTGTTTAG
CCAAACTTTGGTACCAAAGAAAAACCCCAAACCAGGTGAATATGGTCGGGTCATGGCTCAAGAAATTATGATCATTACTC
CTGCTATTTCTAACCTGATTAGAGAAGGGAAAACATCTCAAATTTACTCTGCAATTCAGACCGGGGGTAAATTGGGTATG
CAAACACTAGAAAAAGTTTTAGCTGATTATTATAAAGCCGGAACTATTTCCTTTGAAGCTGCTATGTCTAAAACTTCTAA
ACCCGATGAAATTCAACGTCTCATCGGTGGCGCACCACAAGCAGCAGGTGCAAAACCTGCTGCAGGTGTTGCAAAAGCTC
ATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(49-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6KCX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.014

93.316

0.513
  pilT Pseudomonas stutzeri DSM 10701

54.728

93.316

0.511
  pilT Legionella pneumophila strain Lp02

53.314

92.781

0.495
  pilT Legionella pneumophila strain ERS1305867

53.314

92.781

0.495
  pilT Acinetobacter baylyi ADP1

53.026

92.781

0.492
  pilT Acinetobacter baumannii strain A118

52.738

92.781

0.489
  pilT Acinetobacter baumannii D1279779

52.738

92.781

0.489
  pilT Acinetobacter nosocomialis M2

52.738

92.781

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.425

98.663

0.468
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50

91.444

0.457
  pilT Vibrio cholerae strain A1552

50

91.444

0.457
  pilT Neisseria gonorrhoeae MS11

48.997

93.316

0.457
  pilT Neisseria meningitidis 8013

48.711

93.316

0.455
  pilU Acinetobacter baylyi ADP1

37.845

96.791

0.366
  pilU Pseudomonas stutzeri DSM 10701

38.527

94.385

0.364