Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HGR01_RS21660 Genome accession   NZ_CP063785
Coordinates   5268403..5270418 (-) Length   671 a.a.
NCBI ID   WP_045874067.1    Uniprot ID   A0A0D6KDM9
Organism   Tolypothrix sp. PCC 7712     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5263403..5275418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HGR01_RS21635 (HGR01_21010) - 5263437..5263631 (+) 195 WP_045874072.1 heavy-metal-associated domain-containing protein -
  HGR01_RS21640 (HGR01_21015) bioU 5263884..5264906 (+) 1023 WP_045874071.1 (S)-8-amino-7-oxononanoate synthase BioU -
  HGR01_RS21645 (HGR01_21020) - 5265125..5265682 (-) 558 WP_045874070.1 hypothetical protein -
  HGR01_RS21650 (HGR01_21025) - 5265786..5267006 (-) 1221 WP_045874069.1 type II secretion system F family protein -
  HGR01_RS21655 (HGR01_21030) pilT 5267233..5268357 (-) 1125 WP_045874068.1 type IV pilus twitching motility protein PilT Machinery gene
  HGR01_RS21660 (HGR01_21035) pilB/pilB1 5268403..5270418 (-) 2016 WP_045874067.1 GspE/PulE family protein Machinery gene
  HGR01_RS21665 (HGR01_21040) grpE 5270838..5271584 (+) 747 WP_045874066.1 nucleotide exchange factor GrpE -
  HGR01_RS21670 (HGR01_21045) dnaK 5271917..5273875 (+) 1959 WP_045874065.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 671 a.a.        Molecular weight: 75161.96 Da        Isoelectric Point: 5.1095

>NTDB_id=496802 HGR01_RS21660 WP_045874067.1 5268403..5270418(-) (pilB/pilB1) [Tolypothrix sp. PCC 7712]
MTYSSPQRRSTALTTKTEFSPFGNKLVQTGYVNNEQMRQALIESRKSGTPLTEVLESITGRQLSPELVRQYKKQQLFELK
ILYGVEFLDPEVNQLANTMVGRLIETLIPVDICRRHRLIPLSKHDDQTPPCVLVAMVDPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQQLINQYLDELAIRQKHLEQEKFTDINQDLENLNNLDIDDAPDEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLRQAFDPLPKKIVAAVTARFKIISSLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKICLRILDNSSTQLGLNKLITDQETLDIVKDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGVNISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCPDCRIPYTPTTEELARYGMSASQEVNVTFYKANSLPNEVQADAKAKGQQVCSTCN
GVGYKGRCGVYEVMRITENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAK
RKSGLTCRTCDATLQPEWLDCPYCLTPRFTD

Nucleotide


Download         Length: 2016 bp        

>NTDB_id=496802 HGR01_RS21660 WP_045874067.1 5268403..5270418(-) (pilB/pilB1) [Tolypothrix sp. PCC 7712]
ATGACTTACTCGTCACCGCAACGGCGCAGTACCGCTCTAACCACAAAAACAGAGTTTTCGCCCTTTGGCAACAAGCTAGT
ACAGACTGGCTATGTTAATAACGAACAGATGAGACAGGCACTCATTGAAAGCCGCAAATCTGGCACACCTTTAACGGAAG
TACTAGAGTCAATTACCGGGCGGCAACTCTCACCTGAATTAGTTAGACAATACAAAAAACAGCAGTTATTTGAACTAAAA
ATACTATACGGGGTTGAATTCCTTGATCCGGAAGTCAACCAACTTGCTAATACGATGGTGGGGAGGTTAATTGAAACCCT
CATTCCCGTAGACATTTGTCGTCGCCATCGCTTAATCCCACTATCAAAACATGACGACCAAACCCCACCTTGCGTTTTGG
TGGCGATGGTCGATCCGGATAACCTGGAAGCTTCGGATGACCTCAACCGCATCTTGCGCCCACAAGGTTTAGCTTTGCAG
CGCATGGTAATTACCCAGGAAGATTACCAACAGCTGATTAATCAATACTTAGATGAACTAGCAATTAGGCAAAAACATCT
AGAACAAGAAAAGTTTACAGATATCAATCAAGATTTAGAAAACCTGAATAATCTTGATATAGATGATGCTCCTGATGAGA
TGGAAGCTGACTTAGGCGCAGCAATGAAGGGTGCAGAAGACGCACCAGTTATCAATCTCGTCAATAGAATTTTGGCCAAA
GCCTTGCATGAAAAGGTTTCGGATATTCATATTGAACCGCAGGAAGAAAATCTCCGGATTCGCTTTCGTAAGGATGGGGT
ACTGCGTCAAGCATTTGACCCCTTGCCGAAAAAAATCGTGGCTGCGGTGACAGCCAGGTTTAAAATCATTTCCAGCTTAG
ACATTGCTGAGAGACGTTTACCTCAAGACGGACGTATCCGGCGGATGTTTGAGGGACGTAAAGTGGATTTCCGCGTCAAC
ACCTTACCCAGTCGCTACGGGGAAAAGATTTGTCTGCGGATTTTAGATAACTCCTCTACTCAACTGGGATTGAATAAGCT
GATTACCGACCAAGAAACCTTAGATATTGTTAAGGACATGGTCAGTCGTCCCTTTGGTTTGATTCTAGTTACCGGCCCAA
CTGGCTCTGGTAAAACCACTTCGCTGTATTCAGCATTGTCAGAAAAGAACGATCCCGGAGTTAACATCAGTACAGTAGAA
GACCCAATTGAGTACAGTTTGCCAGGCATCACTCAAGTACAGGTAATTCGGGAAAAAGGACTAGATTTTGCTACCGCTTT
GCGGGCTTTTTTGCGGCAAGACCCAGATGTGTTACTCGTGGGTGAAACCAGAGACAAGGAAACTGCCAAAACAGCGATTG
AGGCAGCCTTAACAGGTCACTTAGTATTAACCACCTTACATACCAATGATGCCCCAGGTGCGATCGCACGTTTGGGAGAA
ATGGGCATTGAACCTTTCATGGTTTCTAGTTCTTTGATTGGTGTGTTGGCACAACGCTTAGTTAGGCGTGTTTGTCCTGA
TTGTCGTATTCCCTATACTCCTACAACCGAAGAACTGGCTCGTTATGGTATGTCTGCTTCTCAGGAAGTGAATGTCACTT
TCTACAAAGCTAACTCTTTGCCCAACGAAGTCCAAGCGGATGCCAAAGCTAAAGGTCAACAAGTATGCTCCACCTGTAAT
GGCGTTGGATATAAAGGACGTTGTGGTGTTTATGAAGTAATGCGAATTACCGAAAACTTGCAAACTCTCATCAACGAAGA
AGCACCAACGGAACGGATTAAGGAAGTAGCAGTAGAAGAAGGGATGAAAACCTTGCTGGCGTACAGCTTAGATTTAGTAC
GTCAAGGCTCCACCACCCTAGAAGAAGTTGAACGAGTCACCTTTACTGATACCGGTTTAGAAGCGGAATTAAAAGCTAAA
CGCAAGAGTGGTCTGACTTGCCGCACTTGTGATGCCACATTACAACCAGAATGGCTAGATTGTCCCTACTGTCTAACACC
TCGCTTTACCGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D6KDM9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.889

100

0.653