Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IH404_RS26760 Genome accession   NZ_CP063780
Coordinates   6106601..6107635 (-) Length   344 a.a.
NCBI ID   WP_122735891.1    Uniprot ID   A0A7S7MTS4
Organism   Pseudomonas sp. OST1909     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6101601..6112635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IH404_RS26730 (IH404_26730) ruvX 6101643..6102080 (+) 438 WP_005792259.1 Holliday junction resolvase RuvX -
  IH404_RS26735 (IH404_26735) pyrR 6102213..6102719 (+) 507 WP_194439480.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  IH404_RS26740 (IH404_26740) - 6102744..6103748 (+) 1005 WP_005792261.1 aspartate carbamoyltransferase catalytic subunit -
  IH404_RS26745 (IH404_26745) - 6103745..6105016 (+) 1272 WP_194439481.1 dihydroorotase -
  IH404_RS26750 (IH404_26750) - 6105136..6105564 (-) 429 WP_122735893.1 TM2 domain-containing protein -
  IH404_RS26755 (IH404_26755) - 6105814..6106452 (+) 639 WP_122735892.1 C40 family peptidase -
  IH404_RS26760 (IH404_26760) pilT 6106601..6107635 (-) 1035 WP_122735891.1 type IV pilus twitching motility protein PilT Machinery gene
  IH404_RS26765 (IH404_26765) - 6107693..6108388 (+) 696 WP_078822556.1 YggS family pyridoxal phosphate-dependent enzyme -
  IH404_RS26770 (IH404_26770) proC 6108414..6109232 (+) 819 WP_194439483.1 pyrroline-5-carboxylate reductase -
  IH404_RS26775 (IH404_26775) - 6109258..6109845 (+) 588 WP_194439485.1 YggT family protein -
  IH404_RS26780 (IH404_26780) - 6110067..6111206 (+) 1140 WP_005792271.1 homoserine O-acetyltransferase -
  IH404_RS26785 (IH404_26785) metW 6111214..6111834 (+) 621 WP_005792273.1 methionine biosynthesis protein MetW -
  IH404_RS26790 (IH404_26790) - 6111856..6112290 (+) 435 WP_005792275.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37567.55 Da        Isoelectric Point: 7.5828

>NTDB_id=496787 IH404_RS26760 WP_122735891.1 6106601..6107635(-) (pilT) [Pseudomonas sp. OST1909]
MDITQLLTASVSRGACDLHLSAGLAPMLRVDGEVWPMDEPVLNAAQLVDLVSPLLNKHQQKDFETSLETDFAFELPGVAR
FRANVFRQNRGIGAVFRAIPTEVQSLEDLGLGEVFQRMAQFPRGLVLVTGPTGSGKSTTLAAMIDYLNRHRRQHILTLED
PIEFIHRPKLALITQRQVHRDTHSFCVALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRL
VDVFPAGEKAMVRSMLSESLQAVVSQVLVKKVGGGRVAAHEIMLGTPAIRNLIREDKVAQMVSAIQTGGALGMKTLDMSL
KALVGAGLIRREEAREKARVPADI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=496787 IH404_RS26760 WP_122735891.1 6106601..6107635(-) (pilT) [Pseudomonas sp. OST1909]
ATGGATATCACGCAATTGCTGACGGCCAGCGTAAGCCGTGGCGCCTGTGACCTGCATTTGTCGGCCGGCCTGGCGCCGAT
GCTGCGAGTCGATGGCGAGGTCTGGCCGATGGATGAGCCGGTGCTGAACGCAGCGCAACTGGTCGATCTGGTGAGCCCTT
TGTTGAATAAACACCAACAAAAGGATTTCGAAACATCTCTTGAAACGGATTTTGCCTTCGAATTGCCGGGAGTCGCACGC
TTTCGGGCGAACGTGTTCCGCCAGAATCGCGGTATAGGCGCGGTGTTTCGAGCCATCCCGACCGAGGTCCAGAGCCTGGA
AGACCTTGGGTTGGGCGAGGTGTTCCAGCGTATGGCGCAGTTCCCCCGTGGCCTGGTGCTGGTCACCGGGCCTACCGGTT
CGGGCAAGTCCACCACGCTGGCGGCGATGATCGATTATCTGAACCGGCACCGGCGCCAGCACATCCTCACGCTTGAAGAC
CCCATCGAATTTATCCACAGGCCGAAACTGGCCCTGATCACCCAGCGCCAGGTGCACCGCGATACCCACAGCTTCTGCGT
GGCGCTGCGCTCGGCCCTGAGGGAAGACCCGGATGTGATCCTGGTGGGCGAATTGCGCGACCTCGAAACCATCCGTCTGG
CCTTGACGGCCGCTGAGACCGGGCACCTGGTGTTTGGCACCCTGCACACTTCATCGGCTGCAAAGACGGTAGACAGGCTG
GTGGACGTCTTCCCGGCCGGGGAAAAGGCCATGGTCCGCTCGATGTTGTCGGAGTCGTTGCAGGCGGTGGTGTCCCAGGT
ACTGGTGAAGAAGGTCGGCGGCGGCCGTGTGGCCGCCCATGAAATCATGCTGGGCACGCCGGCGATTCGCAATCTGATCC
GTGAGGACAAGGTGGCGCAGATGGTGTCGGCAATTCAGACCGGGGGCGCGTTGGGGATGAAGACGCTGGATATGAGTTTG
AAGGCGTTGGTCGGCGCGGGGTTGATCAGGCGGGAAGAGGCGCGGGAGAAGGCGAGGGTGCCTGCAGATATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S7MTS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.093

100

0.721
  pilT Pseudomonas stutzeri DSM 10701

70.349

100

0.703
  pilT Acinetobacter nosocomialis M2

68.915

99.128

0.683
  pilT Acinetobacter baumannii D1279779

68.915

99.128

0.683
  pilT Acinetobacter baumannii strain A118

68.915

99.128

0.683
  pilT Acinetobacter baylyi ADP1

68.035

99.128

0.674
  pilT Legionella pneumophila strain Lp02

63.609

98.256

0.625
  pilT Legionella pneumophila strain ERS1305867

63.609

98.256

0.625
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

63.501

97.965

0.622
  pilT Vibrio cholerae strain A1552

63.501

97.965

0.622
  pilT Neisseria meningitidis 8013

61.471

98.837

0.608
  pilT Neisseria gonorrhoeae MS11

61.176

98.837

0.605
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.491
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

39.42

100

0.395
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381