Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   INQ40_RS10910 Genome accession   NZ_CP063772
Coordinates   2387994..2389091 (+) Length   365 a.a.
NCBI ID   WP_193984498.1    Uniprot ID   A0A7S6ZS55
Organism   Lysobacter sp. H21R4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2382994..2394091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ40_RS10885 (INQ40_10885) - 2383649..2384830 (-) 1182 WP_194340445.1 cystathionine gamma-synthase -
  INQ40_RS10890 (INQ40_10890) - 2384989..2386365 (-) 1377 WP_194340446.1 pyridoxal-phosphate dependent enzyme -
  INQ40_RS10895 (INQ40_10895) - 2386475..2386693 (-) 219 WP_194340447.1 YdcH family protein -
  INQ40_RS10900 (INQ40_10900) - 2386912..2387523 (-) 612 WP_194340448.1 hypothetical protein -
  INQ40_RS10905 (INQ40_10905) - 2387520..2387822 (-) 303 WP_228482004.1 DUF4398 domain-containing protein -
  INQ40_RS10910 (INQ40_10910) pilU 2387994..2389091 (+) 1098 WP_193984498.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INQ40_RS10915 (INQ40_10915) maiA 2389138..2389815 (-) 678 WP_194340450.1 maleylacetoacetate isomerase -
  INQ40_RS10920 (INQ40_10920) - 2389840..2390826 (-) 987 WP_194340451.1 fumarylacetoacetate hydrolase family protein -
  INQ40_RS10930 (INQ40_10930) rpsI 2391030..2391422 (-) 393 WP_193984504.1 30S ribosomal protein S9 -
  INQ40_RS10935 (INQ40_10935) rplM 2391433..2391861 (-) 429 WP_194340452.1 50S ribosomal protein L13 -
  INQ40_RS10940 (INQ40_10940) - 2392040..2393254 (+) 1215 WP_194340453.1 (Fe-S)-binding protein -
  INQ40_RS10945 (INQ40_10945) coq7 2393264..2393908 (+) 645 WP_194340454.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40567.68 Da        Isoelectric Point: 6.3731

>NTDB_id=496706 INQ40_RS10910 WP_193984498.1 2387994..2389091(+) (pilU) [Lysobacter sp. H21R4]
MDIGYFLKLMTEKNASDMFLSTGAPVYIKIEGRLHPLGNTGLPPGMVKKIAYSLMGEGQVPQFERELELNMAYSLPDTGR
FRINVFRQRGEVGMVIRAIRSVIPSIEELQLPQVLKNIILEPRGLVLIVGSTGSGKSTTLASMIDHRNSSVSGHILTIED
PIEYLHRHKRSLVNQREVGLDTHAFGSALKNAMREAPDVILIGEILDQSTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLSLNLRAVISQRLVVGVDGRRVPAAEVLINTPHIRDLIRRGQIHEIKQAMEESLEEGIESFDQCL
FRMQKQGKIDIDEALRAADSRDGLALKFRLSEGADAEHDQYASVF

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=496706 INQ40_RS10910 WP_193984498.1 2387994..2389091(+) (pilU) [Lysobacter sp. H21R4]
ATGGACATCGGCTATTTTCTGAAGCTGATGACGGAGAAGAACGCGTCGGACATGTTCTTGAGCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCCGGCTGCATCCGTTGGGCAATACCGGCCTGCCACCCGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGGCGAAGGCCAGGTGCCGCAGTTCGAGCGAGAGCTGGAGCTCAACATGGCGTACTCGCTGCCCGACACCGGCCGC
TTCCGCATCAACGTGTTCAGGCAACGCGGCGAAGTCGGCATGGTGATCCGTGCGATCCGCAGCGTGATCCCCTCGATCGA
GGAACTGCAGCTGCCGCAGGTGCTCAAGAACATCATCCTGGAGCCGCGCGGCCTGGTCCTCATCGTCGGCTCCACCGGGT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGATCACCGCAACAGCAGCGTCTCCGGCCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACCGGCACAAGCGTTCGCTGGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCGGCAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCTCGACCAGTCGACGATGGAGGCGG
CGATCGCGTTTGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACGATCGAGCGCATC
CTCAACTTCTTCCCCGAGGCGGCGCACAAGAACGTGCTGATGAACCTGTCGCTCAACCTGAGGGCGGTGATTTCCCAGCG
CCTGGTGGTGGGCGTGGACGGGCGCCGGGTGCCTGCGGCGGAGGTTCTGATCAATACCCCGCATATCCGCGATCTGATCC
GGCGCGGCCAGATCCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGAAGGCATCGAATCTTTCGACCAGTGCCTG
TTCCGGATGCAGAAGCAGGGCAAGATCGACATCGACGAGGCCTTGCGCGCGGCCGACTCACGCGACGGGCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGCCGACGCCGAACACGACCAGTACGCGTCGGTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6ZS55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

49.861

98.356

0.49
  pilU Acinetobacter baylyi ADP1

47.899

97.808

0.468
  pilU Vibrio cholerae strain A1552

47.472

97.534

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.349

94.247

0.427
  pilT Pseudomonas aeruginosa PAK

41.716

92.603

0.386
  pilT Pseudomonas stutzeri DSM 10701

41.716

92.603

0.386
  pilT Acinetobacter baumannii strain A118

41.42

92.603

0.384
  pilT Acinetobacter nosocomialis M2

41.42

92.603

0.384
  pilT Acinetobacter baumannii D1279779

41.42

92.603

0.384
  pilT Acinetobacter baylyi ADP1

41.176

93.151

0.384
  pilT Legionella pneumophila strain Lp02

41.124

92.603

0.381
  pilT Legionella pneumophila strain ERS1305867

41.124

92.603

0.381
  pilT Vibrio cholerae strain A1552

41.441

91.233

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

91.233

0.378
  pilT Neisseria meningitidis 8013

39.481

95.068

0.375
  pilT Neisseria gonorrhoeae MS11

39.481

95.068

0.375