Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   INQ43_RS10760 Genome accession   NZ_CP063658
Coordinates   2374098..2375195 (+) Length   365 a.a.
NCBI ID   WP_194036448.1    Uniprot ID   A0A7S6UPP9
Organism   Lysobacter sp. H23M47     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2369098..2380195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ43_RS10735 (INQ43_10735) - 2369755..2370933 (-) 1179 WP_228062497.1 cystathionine gamma-synthase -
  INQ43_RS10740 (INQ43_10740) - 2371090..2372466 (-) 1377 WP_194036444.1 pyridoxal-phosphate dependent enzyme -
  INQ43_RS10745 (INQ43_10745) - 2372576..2372794 (-) 219 WP_194036445.1 YdcH family protein -
  INQ43_RS10750 (INQ43_10750) - 2373014..2373625 (-) 612 WP_194036446.1 hypothetical protein -
  INQ43_RS10755 (INQ43_10755) - 2373622..2373945 (-) 324 WP_228062498.1 DUF4398 domain-containing protein -
  INQ43_RS10760 (INQ43_10760) pilU 2374098..2375195 (+) 1098 WP_194036448.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  INQ43_RS10765 (INQ43_10765) maiA 2375238..2375915 (-) 678 WP_194036449.1 maleylacetoacetate isomerase -
  INQ43_RS10770 (INQ43_10770) - 2375941..2376927 (-) 987 WP_194036450.1 fumarylacetoacetate hydrolase family protein -
  INQ43_RS10780 (INQ43_10780) rpsI 2377131..2377523 (-) 393 WP_193984504.1 30S ribosomal protein S9 -
  INQ43_RS10785 (INQ43_10785) rplM 2377534..2377962 (-) 429 WP_043958007.1 50S ribosomal protein L13 -
  INQ43_RS10790 (INQ43_10790) - 2378142..2379356 (+) 1215 WP_194036451.1 (Fe-S)-binding protein -
  INQ43_RS10795 (INQ43_10795) coq7 2379366..2380010 (+) 645 WP_194034260.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 40504.63 Da        Isoelectric Point: 6.3735

>NTDB_id=496174 INQ43_RS10760 WP_194036448.1 2374098..2375195(+) (pilU) [Lysobacter sp. H23M47]
MDIGYFLKLMTEKNASDMFLSTGAPVYIKIEGRLHPLGNTGLPPGMVKKIAYSLMGDGQVPQFERELELNMAYSLSDAGR
FRINVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKNIILEPRGLVLIVGSTGSGKSTTLASMIDHRNSSVSGHILTIED
PIEYLHRHKRSLVNQREVGLDTHAFGSALKNAMREAPDVILIGEILDQSTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLSLNMKAVISQRLVVGVDGRRVPAAEVLINTPHIRDLIRRGRIHEIKQAMEESLEEGIESFDQCL
FRMQKEGKIDIDEALRAADSRDGLALKFRLSEGADAEHDQYASVF

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=496174 INQ43_RS10760 WP_194036448.1 2374098..2375195(+) (pilU) [Lysobacter sp. H23M47]
ATGGACATCGGCTATTTCTTGAAGCTGATGACGGAGAAGAACGCGTCGGACATGTTCTTGAGCACCGGCGCGCCGGTGTA
CATCAAGATCGAGGGCCGGCTGCATCCGTTGGGAAATACCGGCCTGCCGCCCGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGGCGACGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCTATTCGCTGTCCGATGCGGGTCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGTCGATCGA
GGAACTGCAGCTGCCGCAGGTGCTCAAGAACATCATCCTGGAGCCACGCGGCCTGGTCCTGATCGTCGGCTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCCTCGATGATCGACCACCGCAACAGCAGCGTCTCCGGGCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTCCACCGGCACAAGCGCTCGCTGGTCAACCAGCGTGAGGTCGGTCTGGACACCCACGCCTTCGGCAG
CGCGCTGAAAAACGCGATGCGTGAAGCACCCGACGTGATCCTGATTGGCGAGATCCTGGACCAGTCGACGATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGTCATCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACGATCGAGCGCATC
CTCAATTTCTTCCCCGAGGCGGCGCACAAGAACGTGCTGATGAACCTGTCGCTGAACATGAAGGCGGTGATTTCCCAGCG
CCTGGTGGTCGGCGTGGACGGCCGCCGCGTGCCCGCCGCCGAGGTTCTGATCAACACGCCACACATCCGCGACCTGATCC
GGCGTGGCCGGATCCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGAAGGCATCGAATCCTTCGACCAGTGCCTG
TTCCGGATGCAGAAGGAAGGCAAGATCGATATCGACGAGGCGTTGCGCGCGGCCGACTCACGCGACGGGCTGGCGCTGAA
GTTCCGCCTCTCCGAAGGGGCCGATGCCGAGCACGACCAGTATGCGTCGGTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S6UPP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

49.304

98.356

0.485
  pilU Acinetobacter baylyi ADP1

47.645

98.904

0.471
  pilU Vibrio cholerae strain A1552

47.753

97.534

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.767

94.247

0.422
  pilT Pseudomonas aeruginosa PAK

41.42

92.603

0.384
  pilT Pseudomonas stutzeri DSM 10701

41.42

92.603

0.384
  pilT Acinetobacter baumannii strain A118

41.124

92.603

0.381
  pilT Acinetobacter nosocomialis M2

41.124

92.603

0.381
  pilT Acinetobacter baumannii D1279779

41.124

92.603

0.381
  pilT Legionella pneumophila strain Lp02

41.124

92.603

0.381
  pilT Legionella pneumophila strain ERS1305867

41.124

92.603

0.381
  pilT Acinetobacter baylyi ADP1

40.882

93.151

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

91.233

0.373
  pilT Vibrio cholerae strain A1552

40.841

91.233

0.373
  pilT Neisseria gonorrhoeae MS11

38.905

95.068

0.37
  pilT Neisseria meningitidis 8013

38.905

95.068

0.37