Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IMY97_RS05325 Genome accession   NZ_CP063380
Coordinates   1180921..1181937 (+) Length   338 a.a.
NCBI ID   WP_105879773.1    Uniprot ID   -
Organism   Pectobacterium versatile strain ECC15     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1175921..1186937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMY97_RS05295 (IMY97_05315) hemW 1176615..1177754 (-) 1140 WP_188247231.1 radical SAM family heme chaperone HemW -
  IMY97_RS05300 (IMY97_05320) - 1177747..1178340 (-) 594 WP_039470191.1 XTP/dITP diphosphatase -
  IMY97_RS05305 (IMY97_05325) yggU 1178372..1178662 (-) 291 WP_103942383.1 DUF167 family protein YggU -
  IMY97_RS05310 (IMY97_05330) - 1178659..1179213 (-) 555 WP_010295003.1 YggT family protein -
  IMY97_RS05315 (IMY97_05335) proC 1179263..1180084 (-) 822 WP_107333738.1 pyrroline-5-carboxylate reductase -
  IMY97_RS05320 (IMY97_05340) - 1180186..1180899 (-) 714 WP_010294998.1 YggS family pyridoxal phosphate-dependent enzyme -
  IMY97_RS05325 (IMY97_05345) pilT 1180921..1181937 (+) 1017 WP_105879773.1 type IV pilus twitching motility protein PilT Machinery gene
  IMY97_RS05330 (IMY97_05350) - 1181906..1183738 (-) 1833 WP_105879772.1 diguanylate cyclase -
  IMY97_RS05335 (IMY97_05355) - 1184389..1185960 (-) 1572 WP_103971948.1 methyl-accepting chemotaxis protein -
  IMY97_RS05340 (IMY97_05360) nsrR 1186304..1186729 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36771.99 Da        Isoelectric Point: 6.6280

>NTDB_id=494870 IMY97_RS05325 WP_105879773.1 1180921..1181937(+) (pilT) [Pectobacterium versatile strain ECC15]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTPDQVAQWCDAWLDPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSAQPSLEALHAPPVFSTLLEKPNGLLLITGATGSGKSTTLAAMLGALNDRCDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQTLLPAAHGGRIALYEVLTATAAVSNLIREGKTHQIPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=494870 IMY97_RS05325 WP_105879773.1 1180921..1181937(+) (pilT) [Pectobacterium versatile strain ECC15]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GTTACGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGCCAGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGACCCCGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGCGCGTTAATGCTGCCCGATGGACAG
CGCCTGCGGGTGAACGTGTTTCGCCAGCGTGAGGGGCTGTCTGCGGCGCTGCGCGTTATTCCATCCGCTCAGCCTTCATT
GGAAGCGCTACACGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAACCGAACGGACTGCTCCTGATTACCGGTGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACCGCTGCGATCGCCATGTGATTACGCTGGAG
GACCCGATCGAGTTTATCCATGCTAGCCGACGCTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCGTCGTTTGC
GCAGGCATTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTACGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGCGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCTGGTGAAGAAAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCGGTGGTGACGCA
AACACTTTTGCCTGCGGCACACGGTGGCCGCATTGCGCTTTATGAAGTACTGACCGCCACCGCGGCAGTCAGCAACCTGA
TTCGGGAAGGGAAAACGCACCAGATCCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

51.205

98.225

0.503
  pilT Neisseria gonorrhoeae MS11

50.904

98.225

0.5
  pilT Pseudomonas stutzeri DSM 10701

50.151

97.929

0.491
  pilT Pseudomonas aeruginosa PAK

49.849

97.929

0.488
  pilT Acinetobacter baylyi ADP1

49.849

97.929

0.488
  pilT Acinetobacter baumannii D1279779

49.547

97.929

0.485
  pilT Acinetobacter baumannii strain A118

49.547

97.929

0.485
  pilT Acinetobacter nosocomialis M2

49.547

97.929

0.485
  pilT Legionella pneumophila strain ERS1305867

49.245

97.929

0.482
  pilT Legionella pneumophila strain Lp02

49.245

97.929

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.339

98.521

0.388
  pilU Acinetobacter baylyi ADP1

38.199

95.266

0.364