Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IHJ55_RS03815 Genome accession   NZ_CP063242
Coordinates   867558..868574 (+) Length   338 a.a.
NCBI ID   WP_203470790.1    Uniprot ID   -
Organism   Pectobacterium carotovorum strain XP-13     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 862558..873574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHJ55_RS03785 (IHJ55_03785) hemW 863256..864395 (-) 1140 WP_201963367.1 radical SAM family heme chaperone HemW -
  IHJ55_RS03790 (IHJ55_03790) - 864388..864981 (-) 594 WP_039470191.1 XTP/dITP diphosphatase -
  IHJ55_RS03795 (IHJ55_03795) yggU 865013..865303 (-) 291 WP_010295005.1 DUF167 family protein YggU -
  IHJ55_RS03800 (IHJ55_03800) - 865300..865854 (-) 555 WP_010295003.1 YggT family protein -
  IHJ55_RS03805 (IHJ55_03805) proC 865904..866725 (-) 822 WP_203471236.1 pyrroline-5-carboxylate reductase -
  IHJ55_RS03810 (IHJ55_03810) - 866823..867536 (-) 714 WP_203470789.1 YggS family pyridoxal phosphate-dependent enzyme -
  IHJ55_RS03815 (IHJ55_03815) pilT 867558..868574 (+) 1017 WP_203470790.1 type IV pilus twitching motility protein PilT Machinery gene
  IHJ55_RS03820 (IHJ55_03820) - 868543..870375 (-) 1833 WP_010294991.1 diguanylate cyclase -
  IHJ55_RS03825 (IHJ55_03825) - 870909..872480 (-) 1572 WP_203470791.1 methyl-accepting chemotaxis protein -
  IHJ55_RS03830 (IHJ55_03830) nsrR 872775..873200 (+) 426 WP_010279087.1 nitric oxide-sensing transcriptional repressor NsrR -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36857.10 Da        Isoelectric Point: 6.4218

>NTDB_id=494117 IHJ55_RS03815 WP_203470790.1 867558..868574(+) (pilT) [Pectobacterium carotovorum strain XP-13]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPCLTPDQVAQWCDAWLEPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSVQPSLDALHAPPVFSTLLEKTNGLILITGATGSGKSTTLAAMLGALNDRCDRHVITLE
DPIEFIHTSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQKLLPAEQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=494117 IHJ55_RS03815 WP_203470790.1 867558..868574(+) (pilT) [Pectobacterium carotovorum strain XP-13]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCATCCGCCGGT
GTTACGTGTGGACGGGCGACTACAGCCTGAAAATACCTTACCATGTTTAACACCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTTGAACCGGCTCAGCGCGAGCAACTGCGGCAGGCTGGTCAGGTGGACGGGGCATTAATGCTGCCCGATGGACAA
CGCCTGCGGGTGAATGTGTTTCGCCAGCGGGAAGGGCTGTCTGCGGCGTTGCGTGTTATCCCGTCCGTTCAACCTTCATT
GGATGCACTACATGCGCCACCCGTTTTCTCGACGCTGCTGGAGAAAACGAACGGACTGATCCTGATTACCGGGGCGACGG
GGAGCGGCAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGATCGCTGCGATCGCCATGTGATTACGCTGGAG
GACCCGATCGAGTTTATCCATACCAGCCGACGCTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCGTCGTTTGC
ACAGGCACTACGGGCCGCATTGCGGGAGGATCCCGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACCCGTAGCGCATCGCAGGCGGTCGATCGT
TTGATTGACGTTTTTCCTGGTGAAGAGAAAGCCTATGTTCGCAGCCAGTTAGCCACCTGTTTGCAGGCAGTGGTGACGCA
AAAACTCTTGCCTGCGGAGCAAGGGGGGCGAATCGCGCTTTATGAAGTGCTGACCGCCACCGCGGCGGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAGACGGGAGCCGCTGCGGGAATGCAAACGTTTGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

52.121

97.633

0.509
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

97.633

0.509
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Acinetobacter baylyi ADP1

49.245

97.929

0.482
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.543

99.704

0.414
  pilU Vibrio cholerae strain A1552

38.776

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

39.339

98.521

0.388
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367