Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IHJ54_RS16755 Genome accession   NZ_CP063241
Coordinates   3793482..3794498 (-) Length   338 a.a.
NCBI ID   WP_203449503.1    Uniprot ID   -
Organism   Pectobacterium brasiliense strain ZLMLSHJ5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3788482..3799498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHJ54_RS16740 (IHJ54_16725) nsrR 3788813..3789238 (-) 426 WP_014916532.1 nitric oxide-sensing transcriptional repressor NsrR -
  IHJ54_RS16745 (IHJ54_16730) - 3789582..3791153 (+) 1572 WP_040038285.1 methyl-accepting chemotaxis protein -
  IHJ54_RS16750 (IHJ54_16735) - 3791681..3793513 (+) 1833 WP_203449502.1 sensor domain-containing diguanylate cyclase -
  IHJ54_RS16755 (IHJ54_16740) pilT 3793482..3794498 (-) 1017 WP_203449503.1 type IV pilus twitching motility protein PilT Machinery gene
  IHJ54_RS16760 (IHJ54_16745) - 3794519..3795232 (+) 714 WP_039552871.1 YggS family pyridoxal phosphate-dependent enzyme -
  IHJ54_RS16765 (IHJ54_16750) proC 3795327..3796148 (+) 822 WP_203450575.1 pyrroline-5-carboxylate reductase -
  IHJ54_RS16770 (IHJ54_16755) - 3796197..3796751 (+) 555 WP_203449504.1 YggT family protein -
  IHJ54_RS16775 (IHJ54_16760) yggU 3796748..3797038 (+) 291 WP_010279110.1 DUF167 family protein YggU -
  IHJ54_RS16780 (IHJ54_16765) - 3797056..3797649 (+) 594 WP_014916538.1 XTP/dITP diphosphatase -
  IHJ54_RS16785 (IHJ54_16770) hemW 3797642..3798784 (+) 1143 WP_203449505.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36577.78 Da        Isoelectric Point: 6.8190

>NTDB_id=494102 IHJ54_RS16755 WP_203449503.1 3793482..3794498(-) (pilT) [Pectobacterium brasiliense strain ZLMLSHJ5]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPRLTSDQVAQWCDAWLAPAQREQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRVIPSAQPSLAALQAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMLGALNDSSDRHVITLE
DPIEFIHASRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLATCLQAVVTQRLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCDLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=494102 IHJ54_RS16755 WP_203449503.1 3793482..3794498(-) (pilT) [Pectobacterium brasiliense strain ZLMLSHJ5]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGTCACCCGCCGGT
GCTGCGTGTGGACGGGCGGCTACAGCCTGAAAATACCTTACCACGTTTAACGTCGGATCAGGTGGCACAATGGTGCGATG
CCTGGCTCGCACCCGCTCAGCGCGAGCAACTGCGGCAGGCTGGGCAGGTGGACGGGGCATTAATGCTGCCCGATGGACAA
CGCCTGCGGGTCAATGTATTTCGCCAGCGGGAAGGACTGTCTGCCGCGCTGCGCGTTATTCCGTCCGCTCAGCCTTCATT
GGCGGCGCTACAGGCACCACCCGTTCTCTCGACGCTGCTGGAGAAACCGAACGGGCTGATCCTGATTACCGGTGCGACGG
GGAGCGGTAAATCCACGACGCTGGCGGCCATGCTTGGGGCGTTAAACGACAGTAGCGATCGCCATGTGATTACGCTGGAG
GACCCAATCGAGTTTATCCATGCCAGCCGACGTTGCCTGATTCAACAGCGGGAGATCGGTGCGCACAGCGCATCGTTTGC
GCAGGCGCTACGGGCCGCATTACGGGAGGATCCCGATGTCATTCTGCTGGGAGAATTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGCCATCTGGTGTTGTCGACGCTGCATACACGTAGTGCATCGCAGGCGGTCGATCGT
CTGATTGACGTCTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTAGCTACCTGTTTGCAGGCCGTGGTGACGCA
AAGGTTGCTGCCCGCTGCTCAAGGTGGCCGAATTGCGCTTTATGAAGTGCTGACGGCGACGGCGGCGGTCAGTAACCTGA
TTCGGGAAGGGAAAACGCACCAGCTTCCCGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGTCGGGATGGGCTTATTTCACACAGTTGCGACCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.212

97.633

0.5
  pilT Vibrio cholerae strain A1552

51.212

97.633

0.5
  pilT Neisseria meningitidis 8013

50.301

98.225

0.494
  pilT Neisseria gonorrhoeae MS11

50

98.225

0.491
  pilT Pseudomonas stutzeri DSM 10701

49.245

97.929

0.482
  pilT Pseudomonas aeruginosa PAK

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Acinetobacter baylyi ADP1

48.943

97.929

0.479
  pilT Acinetobacter nosocomialis M2

48.64

97.929

0.476
  pilT Acinetobacter baumannii D1279779

48.64

97.929

0.476
  pilT Acinetobacter baumannii strain A118

48.64

97.929

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

99.704

0.423
  pilU Vibrio cholerae strain A1552

39.067

100

0.396
  pilU Pseudomonas stutzeri DSM 10701

39.64

98.521

0.391
  pilU Acinetobacter baylyi ADP1

38.509

95.266

0.367