Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   IM816_RS14605 Genome accession   NZ_CP063231
Coordinates   3356593..3357702 (+) Length   369 a.a.
NCBI ID   WP_250338633.1    Uniprot ID   -
Organism   Luteibacter flocculans strain EIF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3351593..3362702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM816_RS14585 (IM816_14550) - 3353144..3354514 (-) 1371 WP_072321915.1 pyridoxal-phosphate dependent enzyme -
  IM816_RS14590 (IM816_14555) - 3354718..3355050 (-) 333 WP_343124045.1 YdcH family protein -
  IM816_RS14595 (IM816_14560) - 3355315..3355911 (-) 597 WP_345779950.1 DUF4398 domain-containing protein -
  IM816_RS14600 (IM816_14565) - 3355971..3356498 (-) 528 WP_083527348.1 DUF4398 domain-containing protein -
  IM816_RS14605 (IM816_14570) pilU 3356593..3357702 (+) 1110 WP_250338633.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IM816_RS14610 (IM816_14575) maiA 3357880..3358536 (-) 657 WP_345779951.1 maleylacetoacetate isomerase -
  IM816_RS14615 (IM816_14580) - 3358687..3359868 (+) 1182 WP_250338635.1 phosphoglycerate kinase -
  IM816_RS14620 (IM816_14585) - 3359868..3360518 (+) 651 WP_250338636.1 HAD hydrolase-like protein -
  IM816_RS14625 (IM816_14590) - 3360631..3360981 (+) 351 WP_250338637.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41065.29 Da        Isoelectric Point: 6.5076

>NTDB_id=493984 IM816_RS14605 WP_250338633.1 3356593..3357702(+) (pilU) [Luteibacter flocculans strain EIF3]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPSGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEIGMVIRAIKSEIPSLEELRLPTIFRDLIMEPRGLILVVGATGSGKSTTLAAMLDHRNSNSSGHILTIED
PIEYLHRHKRSIVNQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTDTMEAAISFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRMPATEVLLNTPLIRDMIRRGQIHEVKDAMDRSLQEGMQTFDQSL
YRLYKDGLITLDECLNKADSRDGLALKIRLSEGSSGNEFASSDPYGIGV

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=493984 IM816_RS14605 WP_250338633.1 3356593..3357702(+) (pilU) [Luteibacter flocculans strain EIF3]
GTGGATATCGGTTATTTCCTCAAGCTGATGGTGGACAAGGGGGCGTCGGACATGTTCCTGACGACCGGTGCCCCGGTGAA
CATCAAGGTGGAGGGCAAGCTTTACCCGCTCGGAAATACCGGCCTGCCCAGCGGCATGGTCAAGAAAATCGCCTATTCCC
TGATGGATGAGGGTCAAGTGCCGCAGTTCGAGCGCGACCTCGAACTGAACATGGCTCTGGCGGTGAAGGAGGCGGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAGATCGGCATGGTCATCCGCGCCATCAAGAGCGAGATCCCGTCGCTGGA
AGAACTGCGGCTGCCGACGATCTTCCGCGACCTGATCATGGAGCCGCGCGGCCTGATTCTCGTCGTGGGCGCCACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGACCACCGCAACAGCAACAGCTCGGGTCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCATAAGCGGTCCATCGTGAACCAGCGCGAGGTGGGGCTGGATACCCACAGCTATCACGA
GGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCATGATCGGCGAGATCCGCGACACGGACACCATGGAGGCGG
CGATCTCCTTCTCGGAAACCGGCCATCTCTGCCTGGCGACCCTGCACTCGAACAACGCCGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCCGAGTCGGCGCACAAGAACGTGCTGATGAACCTCGCCCTGAACCTGCGCGCGGTCATCAGCCAGCG
CCTGGTCATCGGCAAGGACGGGCGCCGCATGCCGGCGACCGAGGTGCTGCTCAATACGCCGCTGATCCGCGACATGATCC
GCCGCGGCCAGATCCACGAGGTGAAGGACGCCATGGACCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTC
TATCGCCTCTACAAGGACGGCCTGATCACGCTGGACGAGTGCCTCAACAAGGCCGACTCCCGCGATGGCCTGGCGCTCAA
GATCCGTCTTTCCGAGGGTTCCTCGGGTAACGAGTTCGCGTCGAGCGATCCGTACGGCATCGGGGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.552

98.103

0.496
  pilU Vibrio cholerae strain A1552

49.322

100

0.493
  pilU Acinetobacter baylyi ADP1

49.02

96.748

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.443

99.187

0.431
  pilT Acinetobacter baylyi ADP1

42.9

89.702

0.385
  pilT Pseudomonas aeruginosa PAK

41.42

91.599

0.379
  pilT Pseudomonas stutzeri DSM 10701

41.42

91.599

0.379
  pilT Acinetobacter baumannii strain A118

42.121

89.431

0.377
  pilT Acinetobacter nosocomialis M2

42.121

89.431

0.377
  pilT Acinetobacter baumannii D1279779

42.121

89.431

0.377
  pilT Legionella pneumophila strain Lp02

41.212

89.431

0.369
  pilT Legionella pneumophila strain ERS1305867

41.212

89.431

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.763

91.328

0.363
  pilT Vibrio cholerae strain A1552

39.763

91.328

0.363
  pilT Neisseria gonorrhoeae MS11

38.329

94.038

0.36
  pilT Neisseria meningitidis 8013

38.329

94.038

0.36