Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IM817_RS22240 Genome accession   NZ_CP063229
Coordinates   4639001..4640023 (-) Length   340 a.a.
NCBI ID   WP_219228692.1    Uniprot ID   -
Organism   Serratia marcescens strain LVF3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4634001..4645023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM817_RS22210 (IM817_22215) - 4634802..4635314 (+) 513 WP_219228689.1 SprT family zinc-dependent metalloprotease -
  IM817_RS22215 (IM817_22220) endA 4635416..4636111 (+) 696 WP_219228690.1 deoxyribonuclease I -
  IM817_RS22220 (IM817_22225) rsmE 4636181..4636912 (+) 732 WP_049194742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IM817_RS22225 (IM817_22230) gshB 4636923..4637873 (+) 951 WP_219228691.1 glutathione synthase -
  IM817_RS22230 (IM817_22235) - 4638019..4638582 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  IM817_RS22235 (IM817_22240) ruvX 4638582..4639004 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  IM817_RS22240 (IM817_22245) pilT 4639001..4640023 (-) 1023 WP_219228692.1 type IV pilus twitching motility protein PilT Machinery gene
  IM817_RS22245 (IM817_22250) - 4640044..4640751 (+) 708 WP_060422989.1 YggS family pyridoxal phosphate-dependent enzyme -
  IM817_RS22250 (IM817_22255) proC 4640771..4641592 (+) 822 WP_219228693.1 pyrroline-5-carboxylate reductase -
  IM817_RS22255 (IM817_22260) - 4641624..4642178 (+) 555 WP_219228694.1 YggT family protein -
  IM817_RS22260 (IM817_22265) yggU 4642175..4642468 (+) 294 WP_016930091.1 DUF167 family protein YggU -
  IM817_RS22265 (IM817_22270) - 4642608..4643201 (+) 594 WP_219228695.1 XTP/dITP diphosphatase -
  IM817_RS22270 (IM817_22275) hemW 4643194..4644336 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  IM817_RS22275 (IM817_22280) - 4644372..4644536 (-) 165 Protein_4362 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36345.63 Da        Isoelectric Point: 7.0670

>NTDB_id=493961 IM817_RS22240 WP_219228692.1 4639001..4640023(-) (pilT) [Serratia marcescens strain LVF3]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLSAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLVQQREIGRDSHSFDTALRAALREDPDVILLGELRDVATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLIRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGS

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=493961 IM817_RS22240 WP_219228692.1 4639001..4640023(-) (pilT) [Serratia marcescens strain LVF3]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAGTGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGCGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCGGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGGTCCAGCAGCGGGAGATCGGCCGCGACAGTCACAGCTTCGA
TACGGCGTTGCGCGCTGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAGCTGCGCGATGTTGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATTCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.939

97.059

0.524
  pilT Vibrio cholerae strain A1552

53.939

97.059

0.524
  pilT Acinetobacter baylyi ADP1

50.755

97.353

0.494
  pilT Acinetobacter baumannii strain A118

50.303

97.059

0.488
  pilT Acinetobacter nosocomialis M2

50.303

97.059

0.488
  pilT Acinetobacter baumannii D1279779

50.303

97.059

0.488
  pilT Neisseria meningitidis 8013

49.552

98.529

0.488
  pilT Pseudomonas aeruginosa PAK

50

97.059

0.485
  pilT Neisseria gonorrhoeae MS11

49.547

97.353

0.482
  pilT Legionella pneumophila strain ERS1305867

49.245

97.353

0.479
  pilT Legionella pneumophila strain Lp02

49.245

97.353

0.479
  pilT Pseudomonas stutzeri DSM 10701

49.091

97.059

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.444

100

0.447
  pilU Vibrio cholerae strain A1552

40.432

95.294

0.385
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371