Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IMZ28_RS08490 Genome accession   NZ_CP063164
Coordinates   1724389..1725453 (+) Length   354 a.a.
NCBI ID   WP_197548144.1    Uniprot ID   A0A7M1S531
Organism   Sulfurovum indicum strain ST-419     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1719389..1730453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMZ28_RS08455 (IMZ28_08455) - 1719646..1720665 (-) 1020 WP_197548139.1 sensor histidine kinase KdpD -
  IMZ28_RS08460 (IMZ28_08460) - 1720757..1721188 (-) 432 WP_197548140.1 DUF255 domain-containing protein -
  IMZ28_RS08465 (IMZ28_08465) dapE 1721201..1722295 (-) 1095 WP_197549834.1 succinyl-diaminopimelate desuccinylase -
  IMZ28_RS08470 (IMZ28_08470) - 1722306..1722857 (-) 552 WP_197548141.1 YqiA/YcfP family alpha/beta fold hydrolase -
  IMZ28_RS11005 - 1722860..1723204 (-) 345 WP_232087454.1 rhodanese-like domain-containing protein -
  IMZ28_RS11010 - 1723277..1723624 (-) 348 WP_232087455.1 ferredoxin-thioredoxin reductase catalytic domain-containing protein -
  IMZ28_RS08480 (IMZ28_08480) gatC 1723732..1724022 (+) 291 WP_197548142.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  IMZ28_RS08485 (IMZ28_08485) - 1724032..1724379 (+) 348 WP_197548143.1 arsenate reductase family protein -
  IMZ28_RS08490 (IMZ28_08490) pilT 1724389..1725453 (+) 1065 WP_197548144.1 type IV pilus twitching motility protein PilT Machinery gene
  IMZ28_RS08495 (IMZ28_08495) - 1725511..1726107 (+) 597 WP_197548145.1 CvpA family protein -
  IMZ28_RS08500 (IMZ28_08500) - 1726117..1726563 (+) 447 WP_197548146.1 Fur family transcriptional regulator -
  IMZ28_RS08505 (IMZ28_08505) lysS 1726771..1728411 (+) 1641 WP_197548147.1 lysine--tRNA ligase -
  IMZ28_RS08510 (IMZ28_08510) - 1728621..1729832 (-) 1212 WP_232087456.1 cation:proton antiporter -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39179.20 Da        Isoelectric Point: 6.6709

>NTDB_id=493666 IMZ28_RS08490 WP_197548144.1 1724389..1725453(+) (pilT) [Sulfurovum indicum strain ST-419]
MSIFDIKKLLQSVVAHGASDLHLVSRAEPQIRLDGKLKPVNLPKLKGEDIEEMAYSLITEKQKQRFEEFNELDFALLLPG
IGRFRANYYRTLGDTAAAFRIIPIDIPSLDDLGTPPVFKKLVKREKGLILVTGPTGSGKSTTLAALLNEINLYEQKHIIT
VEDPVEFIHDNKKAVFSHREVGEDTKTFATALKYAMREDPDVILIGEMRDKETIAAALTAAETGHLVFATLHTTSAPGTI
NRIIDVFSGEEQPQVRAMLSSSLIAVIAQSLLPKLGGGRIAAHEIMITNHAIANLIREDKVHQIYSQMQLGQEDTGMQTQ
TQALLKFLKEGKISRDVALQFANKPEELSKAVSY

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=493666 IMZ28_RS08490 WP_197548144.1 1724389..1725453(+) (pilT) [Sulfurovum indicum strain ST-419]
ATGTCGATTTTTGATATAAAAAAACTGCTTCAGAGTGTTGTTGCTCACGGTGCATCTGATCTTCATCTTGTCAGCCGGGC
TGAACCACAGATCAGACTTGACGGGAAACTCAAACCTGTCAATCTCCCGAAGCTGAAGGGTGAAGATATCGAAGAGATGG
CATATTCCCTGATCACAGAAAAACAAAAGCAGCGATTTGAAGAGTTCAATGAACTCGACTTTGCTCTTCTGCTTCCAGGT
ATCGGACGATTCAGAGCAAACTACTACCGGACACTGGGTGACACAGCAGCTGCATTCAGGATCATCCCTATCGATATTCC
GTCACTCGATGATCTTGGTACACCGCCGGTATTTAAAAAACTGGTTAAAAGAGAAAAAGGGCTTATCCTTGTTACCGGTC
CTACCGGCTCAGGTAAATCAACAACACTTGCCGCACTGCTGAACGAGATCAACCTGTATGAACAGAAACACATTATTACG
GTTGAGGATCCGGTAGAGTTCATCCATGATAATAAAAAAGCGGTCTTCTCACATAGAGAGGTAGGAGAAGATACCAAAAC
CTTTGCAACTGCACTGAAATATGCAATGCGTGAAGACCCTGATGTCATCCTTATCGGGGAGATGAGGGATAAGGAAACGA
TTGCAGCAGCACTAACCGCAGCAGAAACCGGACACCTTGTTTTCGCCACACTTCATACCACATCTGCACCAGGAACCATT
AACCGTATTATCGATGTTTTCTCAGGTGAAGAGCAACCACAGGTAAGAGCTATGCTCTCCTCTTCCCTTATTGCTGTTAT
TGCCCAGTCTCTCCTTCCCAAACTTGGTGGGGGACGTATAGCGGCACATGAGATCATGATCACTAACCATGCGATCGCGA
ATCTTATCCGTGAAGACAAGGTTCACCAGATATACTCACAAATGCAGCTGGGACAGGAAGATACCGGAATGCAGACTCAA
ACTCAGGCACTGCTTAAATTCCTCAAAGAAGGCAAGATCAGCAGGGATGTTGCCCTGCAATTTGCGAATAAACCGGAGGA
ACTCTCAAAAGCGGTCTCATACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7M1S531

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

53.333

97.458

0.52
  pilT Acinetobacter baumannii strain A118

52.464

97.458

0.511
  pilT Acinetobacter nosocomialis M2

52.464

97.458

0.511
  pilT Acinetobacter baumannii D1279779

52.464

97.458

0.511
  pilT Pseudomonas aeruginosa PAK

51.884

97.458

0.506
  pilT Acinetobacter baylyi ADP1

51.594

97.458

0.503
  pilT Legionella pneumophila strain ERS1305867

52.507

95.763

0.503
  pilT Legionella pneumophila strain Lp02

52.507

95.763

0.503
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.121

93.22

0.486
  pilT Vibrio cholerae strain A1552

52.121

93.22

0.486
  pilT Neisseria meningitidis 8013

48.991

98.023

0.48
  pilT Neisseria gonorrhoeae MS11

48.991

98.023

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.571

98.87

0.441
  pilU Acinetobacter baylyi ADP1

40.922

98.023

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.462

97.74

0.395
  pilU Vibrio cholerae strain A1552

37.861

97.74

0.37