Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   INQ46_RS03700 Genome accession   NZ_CP063140
Coordinates   774970..775950 (+) Length   326 a.a.
NCBI ID   WP_203437988.1    Uniprot ID   A0A5J2K6D8
Organism   Salmonella enterica subsp. enterica serovar Napoli strain 16-174478     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 769970..780950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INQ46_RS03670 (INQ46_03665) - 770547..771554 (+) 1008 WP_203437987.1 DUF1202 domain-containing protein -
  INQ46_RS03675 (INQ46_03670) hemW 771646..772782 (-) 1137 WP_048590561.1 radical SAM family heme chaperone HemW -
  INQ46_RS03680 (INQ46_03675) - 772775..773368 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  INQ46_RS03685 (INQ46_03680) yggU 773376..773666 (-) 291 WP_001277203.1 DUF167 family protein YggU -
  INQ46_RS03690 (INQ46_03685) - 773663..774229 (-) 567 WP_001094848.1 YggT family protein -
  INQ46_RS03695 (INQ46_03690) - 774248..774952 (-) 705 WP_000997812.1 YggS family pyridoxal phosphate-dependent enzyme -
  INQ46_RS03700 (INQ46_03695) pilT 774970..775950 (+) 981 WP_203437988.1 type IV pilus twitching motility protein PilT Machinery gene
  INQ46_RS03705 (INQ46_03700) - 776080..776766 (+) 687 WP_203437989.1 IclR family transcriptional regulator -
  INQ46_RS03710 (INQ46_03705) ruvX 776813..777229 (-) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  INQ46_RS03715 (INQ46_03710) - 777229..777792 (-) 564 WP_001053173.1 YqgE/AlgH family protein -
  INQ46_RS03720 (INQ46_03715) gshB 778005..778952 (-) 948 WP_000593248.1 glutathione synthase -
  INQ46_RS03725 (INQ46_03720) rsmE 778972..779703 (-) 732 WP_001222489.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  INQ46_RS03730 (INQ46_03725) endA 779780..780487 (-) 708 WP_001650639.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36250.54 Da        Isoelectric Point: 7.8164

>NTDB_id=493267 INQ46_RS03700 WP_203437988.1 774970..775950(+) (pilT) [Salmonella enterica subsp. enterica serovar Napoli strain 16-174478]
MNMEEIVTLSVKHNVSDLHLCNAWPAQWRKQGRMEIAPFTAPDVDRLLLDWLNDVQQYQWRTHGQLDFAVSLSGTRRLRA
SAFKHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREVGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQEGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQRMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=493267 INQ46_RS03700 WP_203437988.1 774970..775950(+) (pilT) [Salmonella enterica subsp. enterica serovar Napoli strain 16-174478]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCA
ATGGCGCAAACAAGGACGCATGGAGATCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGTGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCAAACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAGTCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGCGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGACAGGAGGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGCGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J2K6D8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas aeruginosa PAK

44.954

100

0.451
  pilT Acinetobacter baumannii D1279779

44.954

100

0.451
  pilT Acinetobacter baumannii strain A118

44.954

100

0.451
  pilT Acinetobacter nosocomialis M2

44.648

100

0.448
  pilT Pseudomonas stutzeri DSM 10701

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.245

97.853

0.374
  pilU Pseudomonas stutzeri DSM 10701

35.843

100

0.365
  pilU Acinetobacter baylyi ADP1

36.533

99.08

0.362