Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IIE27_RS04395 Genome accession   NZ_CP063074
Coordinates   899427..900407 (+) Length   326 a.a.
NCBI ID   WP_103928580.1    Uniprot ID   -
Organism   Citrobacter braakii strain JPG1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 894427..905407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IIE27_RS04350 - 894711..894823 (+) 113 Protein_854 DUF1202 family protein -
  IIE27_RS04355 (IIE27_04335) hemW 894849..895985 (-) 1137 WP_149382390.1 radical SAM family heme chaperone HemW -
  IIE27_RS04360 (IIE27_04340) - 895978..896571 (-) 594 WP_016154402.1 XTP/dITP diphosphatase -
  IIE27_RS04365 (IIE27_04345) yggU 896579..896869 (-) 291 WP_005123286.1 DUF167 family protein YggU -
  IIE27_RS04370 (IIE27_04350) - 896866..897432 (-) 567 WP_181635209.1 YggT family protein -
  IIE27_RS04375 (IIE27_04355) - 897451..898155 (-) 705 WP_047414022.1 YggS family pyridoxal phosphate-dependent enzyme -
  IIE27_RS04380 (IIE27_04360) - 898173..898298 (+) 126 Protein_860 type IV pili twitching motility protein PilT -
  IIE27_RS04385 (IIE27_04365) - 898814..899233 (+) 420 WP_085055767.1 DUF3828 domain-containing protein -
  IIE27_RS04390 (IIE27_04370) - 899239..899409 (-) 171 Protein_862 YggS family pyridoxal phosphate enzyme -
  IIE27_RS04395 (IIE27_04375) pilT 899427..900407 (+) 981 WP_103928580.1 type IV pilus twitching motility protein PilT Machinery gene
  IIE27_RS04400 (IIE27_04380) ruvX 900404..900820 (-) 417 WP_003825428.1 Holliday junction resolvase RuvX -
  IIE27_RS04405 (IIE27_04385) - 900820..901383 (-) 564 WP_016154395.1 YqgE/AlgH family protein -
  IIE27_RS04410 (IIE27_04390) gshB 901560..902507 (-) 948 WP_181635210.1 glutathione synthase -
  IIE27_RS04415 (IIE27_04395) rsmE 902520..903251 (-) 732 WP_016154393.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  IIE27_RS04420 (IIE27_04400) endA 903327..904034 (-) 708 WP_016154392.1 deoxyribonuclease I -
  IIE27_RS04425 (IIE27_04405) - 904129..904626 (-) 498 WP_016157438.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35880.90 Da        Isoelectric Point: 6.1727

>NTDB_id=492752 IIE27_RS04395 WP_103928580.1 899427..900407(+) (pilT) [Citrobacter braakii strain JPG1]
MNMEEIVALSVKHNVSDLHLCNAWPARWRIGGKVEIAPFTPPDVENLLTCWLSEQQQVQWREQGQIDFALTLADSRRLRA
SAFAHQQGTSLALRLLPLECPRLDDLQTPEAIPELLHGENGLVLVTGATGSGKSTTLAAMVEYLNQHIAGHILTLEDPIE
YRYTSQRCLIQQREVGVHCASFAAGLRGALREDPDVILLGELRDVETIRLALTAAETGHLVLATLHTRGAAQAIARLVDT
FPAQEKDPVRNQLADSLRAVLSQKLEEDKQGGRVALFELLVNTPAVGNLIREGKTHQLPGVIQTGQQTGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=492752 IIE27_RS04395 WP_103928580.1 899427..900407(+) (pilT) [Citrobacter braakii strain JPG1]
ATGAATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAATGCATGGCCTGCGCG
CTGGCGCATAGGTGGAAAAGTCGAAATAGCACCATTTACTCCCCCTGACGTGGAGAATCTGCTGACGTGCTGGCTCAGTG
AGCAACAACAGGTACAGTGGCGGGAACAGGGGCAGATTGATTTTGCCCTTACGCTGGCGGACTCCCGGCGTCTACGCGCC
AGCGCATTTGCTCATCAGCAGGGAACCTCGCTGGCGCTAAGATTGCTACCGCTTGAATGTCCTCGTTTAGACGATCTTCA
GACCCCGGAGGCCATACCTGAACTGCTGCACGGTGAAAATGGATTAGTTCTGGTCACTGGAGCCACCGGCAGCGGTAAAT
CAACCACCCTGGCGGCGATGGTGGAGTACCTTAATCAACATATTGCGGGGCATATTCTGACGCTGGAAGATCCGATTGAA
TATCGCTACACCAGCCAGCGTTGTCTTATTCAACAGCGGGAGGTGGGCGTACACTGCGCTTCTTTTGCTGCCGGTTTGCG
CGGCGCGTTACGCGAAGATCCCGACGTCATTTTGCTGGGCGAGCTGCGCGACGTGGAAACTATTCGTTTGGCCTTAACGG
CAGCGGAGACCGGGCATCTGGTGTTGGCAACCTTACATACGCGAGGGGCGGCGCAGGCCATCGCGCGGCTGGTGGACACC
TTTCCCGCTCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCAGACAGCCTGCGGGCGGTACTTTCGCAAAAGCTGGAGGA
AGATAAGCAGGGAGGACGCGTGGCGCTATTCGAGCTGCTCGTCAACACGCCCGCCGTGGGTAATTTGATCCGCGAAGGGA
AAACGCATCAGCTACCCGGTGTGATTCAAACCGGGCAGCAGACAGGTATGCAGACGTTTGCACAAAGTCAGCAGCAGCGC
CAGGCGCAGGGGCGACTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(56-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Legionella pneumophila strain ERS1305867

47.484

97.546

0.463
  pilT Legionella pneumophila strain Lp02

47.484

97.546

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

45.872

100

0.46
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baumannii D1279779

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Acinetobacter nosocomialis M2

45.566

100

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.271

97.239

0.411
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362