Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   IMO23_RS04300 Genome accession   NZ_CP062919
Coordinates   897069..898106 (-) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain Res13-Abat-PEA21-P4-01-A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 858216..921538 897069..898106 within 0


Gene organization within MGE regions


Location: 858216..921538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMO23_RS04115 (IMO23_04115) - 858851..860476 (-) 1626 WP_000834616.1 NAD+ synthase -
  IMO23_RS04120 (IMO23_04120) - 860861..861421 (-) 561 WP_001048355.1 hypothetical protein -
  IMO23_RS04125 (IMO23_04125) - 861512..862372 (-) 861 WP_002059770.1 pirin family protein -
  IMO23_RS04130 (IMO23_04130) hemJ 862543..862995 (-) 453 WP_000339889.1 protoporphyrinogen oxidase HemJ -
  IMO23_RS04135 (IMO23_04135) - 863014..864243 (-) 1230 WP_000832710.1 beta-ketoacyl-ACP synthase II -
  IMO23_RS04140 (IMO23_04140) - 864540..865865 (+) 1326 WP_000160010.1 EcsC family protein -
  IMO23_RS04145 (IMO23_04145) rpsL 866066..866440 (+) 375 WP_000246374.1 30S ribosomal protein S12 -
  IMO23_RS04150 (IMO23_04150) rpsG 866600..867070 (+) 471 WP_001138055.1 30S ribosomal protein S7 -
  IMO23_RS04155 (IMO23_04155) fusA 867249..869387 (+) 2139 WP_000113824.1 elongation factor G -
  IMO23_RS04160 (IMO23_04160) tuf 869482..870672 (+) 1191 WP_001029610.1 elongation factor Tu -
  IMO23_RS04165 (IMO23_04165) - 870876..871757 (+) 882 WP_002058580.1 metal-dependent hydrolase -
  IMO23_RS04170 (IMO23_04170) - 871999..872874 (+) 876 WP_016654330.1 metal-dependent hydrolase -
  IMO23_RS04175 (IMO23_04175) rimI 872982..873437 (+) 456 WP_002058576.1 ribosomal protein S18-alanine N-acetyltransferase -
  IMO23_RS04180 (IMO23_04180) - 873482..874297 (-) 816 WP_000844343.1 arginyltransferase -
  IMO23_RS04185 (IMO23_04185) aat 874316..875047 (-) 732 WP_002058572.1 leucyl/phenylalanyl-tRNA--protein transferase -
  IMO23_RS04190 (IMO23_04190) trxB 875167..876114 (-) 948 WP_001276144.1 thioredoxin-disulfide reductase -
  IMO23_RS04195 (IMO23_04195) - 876384..879416 (+) 3033 WP_002058569.1 DNA translocase FtsK -
  IMO23_RS04200 (IMO23_04200) rhtC 879566..880186 (+) 621 WP_000959256.1 threonine export protein RhtC -
  IMO23_RS04205 (IMO23_04205) - 880229..880516 (-) 288 WP_001218560.1 PA4642 family protein -
  IMO23_RS04210 (IMO23_04210) minE 880600..880872 (-) 273 WP_000896934.1 cell division topological specificity factor MinE -
  IMO23_RS04215 (IMO23_04215) minD 880875..881687 (-) 813 WP_001074362.1 septum site-determining protein MinD -
  IMO23_RS04220 (IMO23_04220) minC 881758..882480 (-) 723 WP_000763675.1 septum site-determining protein MinC -
  IMO23_RS04225 (IMO23_04225) - 882602..883648 (-) 1047 WP_002058587.1 hypothetical protein -
  IMO23_RS04230 (IMO23_04230) - 883665..884591 (-) 927 WP_000100975.1 acyltransferase -
  IMO23_RS04235 (IMO23_04235) - 884836..885489 (+) 654 WP_001202415.1 OmpA family protein -
  IMO23_RS04240 (IMO23_04240) rep 885683..887722 (+) 2040 WP_000093035.1 DNA helicase Rep -
  IMO23_RS04245 (IMO23_04245) dut 887747..888199 (+) 453 WP_000868152.1 dUTP diphosphatase -
  IMO23_RS04250 (IMO23_04250) - 888399..889817 (+) 1419 WP_001102831.1 phosphomannomutase/phosphoglucomutase -
  IMO23_RS04255 (IMO23_04255) argB 889832..890740 (+) 909 WP_001135419.1 acetylglutamate kinase -
  IMO23_RS04260 (IMO23_04260) - 890861..891643 (+) 783 WP_000890282.1 GNAT family N-acetyltransferase -
  IMO23_RS04265 (IMO23_04265) - 891752..892600 (+) 849 WP_000336554.1 class II glutamine amidotransferase -
  IMO23_RS04270 (IMO23_04270) - 892853..893308 (+) 456 WP_000782976.1 bacteriohemerythrin -
  IMO23_RS04275 (IMO23_04275) - 893461..894528 (+) 1068 WP_195707712.1 hypothetical protein -
  IMO23_RS04280 (IMO23_04280) - 894528..894851 (+) 324 WP_000442589.1 RnfH family protein -
  IMO23_RS04285 (IMO23_04285) - 894906..895304 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  IMO23_RS04290 (IMO23_04290) fur 895416..895853 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  IMO23_RS04295 (IMO23_04295) pilU 895923..897041 (-) 1119 WP_000347036.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  IMO23_RS04300 (IMO23_04300) pilT 897069..898106 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  IMO23_RS04305 (IMO23_04305) - 898233..898925 (+) 693 WP_001108520.1 YggS family pyridoxal phosphate-dependent enzyme -
  IMO23_RS04310 (IMO23_04310) - 899127..902723 (-) 3597 WP_002058592.1 AAA family ATPase -
  IMO23_RS04315 (IMO23_04315) - 902733..904001 (-) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  IMO23_RS04320 (IMO23_04320) - 904166..904639 (+) 474 WP_000152331.1 OsmC family protein -
  IMO23_RS04325 (IMO23_04325) - 904711..905088 (-) 378 WP_001059432.1 VOC family protein -
  IMO23_RS04330 (IMO23_04330) - 905469..905921 (+) 453 WP_000161637.1 ABZJ_00895 family protein -
  IMO23_RS04335 (IMO23_04335) - 906009..906914 (+) 906 WP_001039368.1 TIGR01777 family oxidoreductase -
  IMO23_RS04340 (IMO23_04340) - 906906..908021 (-) 1116 WP_000546680.1 FAD-binding oxidoreductase -
  IMO23_RS04345 (IMO23_04345) hemB 908047..909060 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  IMO23_RS04350 (IMO23_04350) - 909203..909700 (+) 498 WP_002156418.1 thioesterase family protein -
  IMO23_RS04355 (IMO23_04355) - 909970..911121 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  IMO23_RS04360 (IMO23_04360) - 911128..912651 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  IMO23_RS04365 (IMO23_04365) ggt 912955..914940 (+) 1986 WP_002058570.1 gamma-glutamyltransferase -
  IMO23_RS04375 (IMO23_04375) - 915640..916869 (+) 1230 WP_151000667.1 tyrosine-type recombinase/integrase -
  IMO23_RS04380 (IMO23_04380) - 917017..917784 (+) 768 WP_049590698.1 hypothetical protein -
  IMO23_RS04385 (IMO23_04385) - 917994..918209 (+) 216 WP_000153154.1 hypothetical protein -
  IMO23_RS04390 (IMO23_04390) - 918212..918421 (+) 210 WP_001016479.1 helix-turn-helix domain-containing protein -
  IMO23_RS04395 (IMO23_04395) - 918418..918972 (+) 555 WP_001988245.1 BRO family protein -
  IMO23_RS04400 (IMO23_04400) - 918965..919330 (+) 366 WP_039098651.1 hypothetical protein -
  IMO23_RS19305 - 919343..919645 (+) 303 WP_000787401.1 hypothetical protein -
  IMO23_RS04405 (IMO23_04405) - 919760..920038 (+) 279 WP_001064707.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=491968 IMO23_RS04300 WP_000355489.1 897069..898106(-) (pilT) [Acinetobacter baumannii strain Res13-Abat-PEA21-P4-01-A]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=491968 IMO23_RS04300 WP_000355489.1 897069..898106(-) (pilT) [Acinetobacter baumannii strain Res13-Abat-PEA21-P4-01-A]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCTGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CGGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACATCAATCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTAG
CATTAACTGCTGCCGAAACAGGTCACTTGGTGTTTGGTACGCTCCACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCTGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCGCAAATGTACTCAGCCATTCAAACTGGTGCCAATCATGGCATGACCACACTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTAATTAGCCCGCAAACGGCTCGTACTGCAGCAAAACAGCCTGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1
  pilT Acinetobacter baumannii D1279779

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388